Auxotrophic <i>Lactobacillus</i> Expressing Porcine Rotavirus VP4 Constructed Using CRISPR-Cas9D10A System Induces Effective Immunity in Mice by Hailin Zhang, Haiyuan Zhao, Yuliang Zhao, Ling Sui, Fengsai Li, Huijun Zhang, Jiaxuan Li, Yanping Jiang, Wen Cui, Guojie Ding, Han Zhou, Li Wang, Xinyuan Qiao, Lijie Tang, Xiaona Wang, Yijing Li

“…However, the difficulty in knocking out specific genes, inserting foreign genes, and the residues of antibiotic selection markers are major challenges for the oral vaccination of LAB. …”
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miRNA-21, which disrupts metabolic reprogramming to facilitate CD4+ T cell polarization toward the Th2 phenotype, accelerates arsenite-induced hepatic fibrosis by Jing Sun, Meng Wu, Li Wang, Peiwen Wang, Tian Xiao, Suhua Wang, Qizhan Liu

“…Consistent with the results in vitro, miR-21 knock-out in mice reversed hepatic fibrosis and attenuated the levels of GATA3 and IL-13 induced by arsenite. …”
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BRG1 is involved in vascular calcification in chronic renal disease via autophagy of vascular smooth muscle cells by Yunlin Feng, Meidie Yu, Chan Wang, Juhua Xia, Liming Huang, Yun Tang, Qiong Xiao, Lei Pu, Li Wang, Guisen Li, Yi Li

“…Autophagosome formation was measured in high phosphorus stimulated RASMCs with and without BRG1 knock-down. We also detected the coexistence of BGR1 and exosomes, and measured the circulatory levels of BRG1 in the hemodialysis patients. …”
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Predicting and overcoming resistance to CDK9 inhibitors for cancer therapy by Chen Hu, Lijuan Shen, Fengming Zou, Yun Wu, Beilei Wang, Aoli Wang, Chao Wu, Li Wang, Jing Liu, Wenchao Wang, Qingsong Liu

“…Through genomic sequencing, we identified in the kinase domain of CDK9 a mutation L156F, which is also a coding SNP in the CDK9 gene. By knocking in L156F into cancer cells using CRISPR/Cas9, we found that single CDK9 L156F could drive the resistance to CDK9 inhibitors, not only ATP competitive inhibitor but also PROTAC degrader. …”
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Pyrroloquinoline quinone modulates YAP-related anti-ferroptotic activity to protect against myocardial hypertrophy by Jiabin Zhou, Jiabin Zhou, Tao Yu, Tao Yu, Gujie Wu, Gujie Wu, Peng Xu, Peng Xu, Chen Wang, Yiling Su, Yiling Su, Li Wang, Li Wang, Qi Lu, Qi Lu

“…The use of a specific siRNA construct to knock down YAP expression in vitro further confirmed the ability of PQQ to protect against myocardial hypertrophy at least in part through anti-ferroptotic mechanisms.Conclusion: PQQ can regulate the pathogenesis of myocardial hypertrophy through the induction of YAP-related anti-ferroptotic activity, highlighting the potential value of PQQ as a novel therapeutic agent capable of slowing or preventing the progression of myocardial hypertrophy and thus delaying the onset of heart failure.…”
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CRISPR/Cas9-Mediated Mutagenesis of <i>Antennapedia</i> in <i>Spodoptera frugiperda</i> by Congke Wang, Te Zhao, Xiaolong Liu, Tianliang Li, Leiming He, Qinqin Wang, Li Wang, Lin Zhou

“…To functionally disrupt <i>SfAntp</i>, two sgRNA sites were designed at the first exon of <i>SfAntp</i> and the gene was knocked out by CRISPR/Cas9 via microinjection. The results showed that the deletion of <i>SfAntp</i> produced a mutant phenotype of thoracic fusion, thoracic leg defect, leg-like protrusions between the head and thoracic segments and pupation deformity. …”
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Intervening in hnRNPA2B1-mediated exosomal transfer of tumor-suppressive miR-184-3p for tumor microenvironment regulation and cancer therapy by Xueqing Zhou, Yiling Hong, Yupeng Liu, Li Wang, Xuan Liu, Yi Li, Hong Yuan, Fuqiang Hu

“…Overexpressing miR-184-3p in tumor cells and simultaneously knocking down hnRNPA2B1 to block its secretion through exosomes could effectively inhibit tumor growth and metastasis. …”
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Overexpression of PKMYT1 associated with poor prognosis and immune infiltration may serve as a target in triple-negative breast cancer by Huihui Li, Li Wang, Wei Zhang, Youting Dong, Yefeng Cai, Yefeng Cai, Xiaoli Huang, Xiaoli Huang, Xubin Dong

“…TNBC cell lines’ proliferation, migration, and invasion were inhibited when PKMYT1 expression was knock-down. Besides, the down-regulation of PKMYT1 induced apoptosis in vitro. …”
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The Structure and Function of Biomaterial Endolysin EFm1 from <i>E. faecalis</i> Phage by Xuerong Zhou, Xiaotao Zeng, Li Wang, Yanhui Zheng, Guixiang Zhang, Wei Cheng

“…Furthermore, EFm1 exhibited no significant activity when expressed alone in vivo, and IME-EF1 lytic activity decreased when <i>efm1</i> was knocked down. These findings provide valuable insights into the molecule mechanism of a potential functional biomaterial for the treatment of the disease caused by the opportunistic pathogen <i>E. faecalis</i>.…”
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The effects of restraint stress on ceramide metabolism disorders in the rat liver: the role of CerS6 in hepatocyte injury by Yichang Liu, Zhaoling Sun, Qiuli Sun, Li Wang, Chuan Wang, Yingmin Li, Chunling Ma, Weibo Shi, Guozhong Zhang, Yiming Dong, Xiaojing Zhang, Bin Cong

“…Furthermore, CORT induced sequential phosphorylation of AMPK and p38 MAPK proteins, and inhibition of the p38 MAPK pathway using SB203580 mitigated the CORT-induced elevation in CerS6 protein. Knocking down CerS6 in hepatocytes inhibited both the increase in C16:0 ceramide and the release of mitochondrial cytochrome c induced by CORT. …”
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Low‐dose aspirin can inhibit exosomal release induced by radiotherapy in breast cancer and attenuate its inhibitory effect on NK cell proliferation by Li Wang, Zaoxiu Hu, Ceshi Chen, Ting Chen, Zhihong Yao, Wenhui Li, Zuozhang Yang

“…Low doses of aspirin inhibited exosome release from BT549 and 4T1‐Luc cells and alleviated the inhibitory effect of BC cell exosomes on NK cell proliferation. In addition, knocking down Rab27a reduced the protein levels of exosome‐related and secretion‐related genes in BC cells, further enhancing the promotive effect of aspirin on NK cell proliferation, while overexpressing Rab27a had the opposite effect. …”
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