Whole Genome Sequencing and CRISPR/Cas9 Gene Editing of Enterotoxigenic <i>Escherichia coli</i> BE311 for Fluorescence Labeling and Enterotoxin Analyses by Shuang Lu, Ting Tao, Yating Su, Jia Hu, Li Zhang, Guoliang Wang, Xiangyu Li, Xiaohua Guo

“…Subsequently, the fluorescent gene <i>mCherry</i> was successfully knocked into the genome of <i>E. coli</i> BE311 by CRISPR/Cas9. …”
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Mannose-Binding Lectin Reduces Oxidized Low-Density Lipoprotein Induced Vascular Endothelial Cells Injury by Inhibiting LOX1-ox-LDL Binding and Modulating Autophagy by Xuelian Zhou, Xuefeng Chen, Li Zhang, Jinna Yuan, Hu Lin, Mingqiang Zhu, Xiaoqin Xu, Guanping Dong, Junfen Fu, Wei Wu

“…Both LOX1 knock-down and MBL over-expression can ameliorate EC autophagy and cell injury. …”
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Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort <i>Marchantia polymorpha</i> under Blue Light by Li Zhang, Tianhong Li, Shengzhong Su, Hao Peng, Sudi Li, Ke Li, Luyao Ji, Yaoyun Xing, Junchuan Zhang, Xinglin Du, Mingdi Bian, Yuying Liao, Zhenming Yang, Zecheng Zuo

“…To study this problem, we conducted a study in the liverwort <i>Marchantia polymorpha</i> and obtained a MpSPA knock-out mutant, in which Mp<i>spa</i> exhibits the phenotype of an increased percentage of individuals with asymmetrical thallus growth, similar to MpCRY knock-out mutant. …”
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Roles of a Cryptochrome in Carbon Fixation and Sucrose Metabolism in the Liverwort <i>Marchantia polymorpha</i> by Tianhong Li, Li Zhang, Shengzhong Su, Sudi Li, Junchuan Zhang, Zhenming Yang, Zecheng Zuo

“…In this study, we investigated functions of a single-copy ortholog of cryptochrome (<i>MpCRY</i>) in the liverwort <i>Marchantia polymorpha.</i> Knock-out of Mp<i>CRY</i> showed that a large number of the mutant plants exhibited asymmetric growth of thalli under blue light. …”
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A Ser252Trp mutation in fibroblast growth factor receptor 2 (FGFR2) mimicking human Apert syndrome reveals an essential role for FGF signaling in the regulation of endochondral bon... by Peng Chen, Li Zhang, Tujun Weng, Shichang Zhang, Shijin Sun, Mingtao Chang, Yang Li, Bo Zhang, Lianyang Zhang

“…We utilized a Fgfr2(+/S252W) mouse (a knock-in mouse model mimicking human AS) to demonstrate decreased bone mass due to reduced trabecular bone volume, reduced bone mineral density, and shortened growth plates in the long bones. …”
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Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle by Yuefang Zhao, Lei Yang, Guanghua Su, Zhuying Wei, Xuefei Liu, Lishuang Song, Chao Hai, Di Wu, Zhenting Hao, Yunxi Wu, Li Zhang, Chunling Bai, Guangpeng Li

“…Herein, the myostatin gene (<i>MSTN</i>), a negative regulator of skeletal muscle development, was knocked out by CRISPR/Cas9 technology. Eight <i>MSTN</i> gene-edited bull calves (MT) were born, and six of them are well-developed. …”
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Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy by Jiling Feng, Anahitasadat Mansouripour, Zhichao Xi, Li Zhang, Gang Xu, Hua Zhou, Hongxi Xu

“…Additionally, we revealed that NJXA induced lysosome degradation and colocalization of mitochondria and autophagosomes, which was attenuated by knocking down ATG7, the key regulator of mitophagy. …”
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The Receptor-like Kinase TaCRK-7A Inhibits <i>Fusarium pseudograminearum</i> Growth and Mediates Resistance to Fusarium Crown Rot in Wheat by Tianci Wu, Feilong Guo, Gangbiao Xu, Jinfeng Yu, Li Zhang, Xuening Wei, Xiuliang Zhu, Zengyan Zhang

“…<i>TaCRK-7A</i> transcript abundance was elevated after <i>F. pseudograminearum</i> attack and was positively related to FCR-resistance levels of wheat cultivars. Intriguingly, knocking down of <i>TaCRK-7A</i> transcript increased susceptibility of wheat to FCR and decreased transcript levels of defense-marker genes in wheat. …”
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Downregulation of miR-574-5p inhibits HK-2 cell viability and predicts the onset of acute kidney injury in sepsis patients by Shanshan Liu, Lishu Zhao, Li Zhang, Lujun Qiao, Shufang Gao

“…Conclusion Overexpression of miR-574-5p in cultured HK-2 cells increases cell viability and knocked-down expression of miR-574-5p decreases cell viability. …”
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Necroptosis of macrophage is a key pathological feature in biliary atresia via GDCA/S1PR2/ZBP1/p-MLKL axis by Shen Yang, Na Chang, Weiyang Li, Ting Yang, Renmin Xue, Jing Liu, Li Zhang, Xingfeng Yao, Yajun Chen, Huanmin Wang, Lin Yang, Jinshi Huang, Liying Li

“…Finally, after selectively knocking down of macrophage S1pr2 in vivo, ZBP1/p-MLKL-mediated necroptosis was decreased, and further collagen deposition was markedly attenuated in BDL mice. …”
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Multi-Omics Analysis Reveals the Potential Effects of Maternal Dietary Restriction on Fetal Muscle Growth and Development by Xinyue Wang, Mingyu Shang, Wenping Hu, Li Zhang

“…The multi-omics analysis revealed that the <i>TEAD1</i> gene was in the Hippo signaling pathway, the muscle marker genes <i>MYF5</i> and <i>MyoG</i> were significantly down-regulated, and the <i>TEAD1</i> binding of the down-regulated <i>VGLL3</i> gene might be potential mechanisms affecting myofiber differentiation and maturation. Knocking down the <i>CDK2</i> gene could inhibit the proliferation of primary embryonic myoblasts, and the expression levels of cell cycle regulatory factors <i>CDK4</i> and <i>CDK6</i> were significantly changed. …”
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