Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis by Limmathurotsakul, D, Golding, N, Dance, D, Messina, J, Pigott, D, Moyes, C, Rolim, D, Bertherat, E, Day, N, Peacock, S, Hay, S

“…</p> <p>Our estimates suggest that melioidosis is severely underreported in the 45 countries in which it is known to be endemic and that melioidosis is probably endemic in a further 34 countries that have never reported the disease. …”

Phenotypic and functional characterization of human memory T cell responses to Burkholderia pseudomallei by Tippayawat, P, Saenwongsa, W, Mahawantung, J, Suwannasaen, D, Chetchotisakd, P, Limmathurotsakul, D, Peacock, S, Felgner, P, Atkins, H, Titball, R, Bancroft, G, Lertmemongkolchai, G

“…Background Infection with the Gram-negative bacterium Burkholderia pseudomallei is an important cause of community-acquired lethal sepsis in endemic regions in southeast Asia and northern Australia and is increasingly reported in other tropical areas. …”

Emerging of Melioidosis in Indonesia by Tauran, P, Sennang, N, Rusli, B, Wiersinga, W, Dance, D, Arif, M, Limmathurotsakul, D

“…Melioidosis is known to be highly endemic in parts of southeast Asia and northern Australia; however, cases are rarely reported in Indonesia. …”

A call to action: time to recognise melioidosis as a neglected tropical disease by Savelkoel, J, Dance, DAB, Currie, BJ, Limmathurotsakul, D, Wiersinga, WJ

“…Fortunately, the impact of melioidosis in a region can be reduced once awareness is established of its known or suspected endemicity. In this Personal View, we provide evidence in support of official recognition of melioidosis as an NTD. …”

Consensus guidelines for dosing of amoxicillin-clavulanate in melioidosis. by Cheng, A, Chierakul, W, Chaowagul, W, Chetchotisakd, P, Limmathurotsakul, D, Dance, D, Peacock, S, Currie, B

“…Melioidosis is an infectious disease endemic to northern Australia and Southeast Asia. …”

Short report: Consensus guidelines for dosing of amoxicillin-clavulanate in melioidosis by Cheng, A, Chierakul, W, Chaowagul, W, Chetchotisakd, P, Limmathurotsakul, D, Dance, D, Peacock, S, Currie, B

“…Melioidosis is an infectious disease endemic to northern Australia and Southeast Asia. …”

Clinical epidemiology of septic arthritis caused by Burkholderia pseudomallei and other bacterial pathogens in northeast Thailand by Teparrukkul, P, Nilsakul, J, Dunachie, S, Limmathurotsakul, D

“…Melioidosis, a serious infectious disease caused by the Gram-negative bacillus Burkholderia pseudomallei, is highly endemic in South and Southeast Asia and northern Australia. …”

Prevalence of melioidosis in patients with suspected pulmonary tuberculosis and sputum smear negative for acid-fast bacilli in northeast Thailand. by Suntornsut, P, Kasemsupat, K, Silairatana, S, Wongsuvan, G, Jutrakul, Y, Wuthiekanun, V, Day, N, Peacock, S, Limmathurotsakul, D

“…We propose that in melioidosis-endemic areas, residual sputum from AFB testing should be routinely cultured for B. pseudomallei.…”

Melioidosis diagnostic workshop, 2013. by Hoffmaster, A, AuCoin, D, Baccam, P, Baggett, H, Baird, R, Bhengsri, S, Blaney, D, Brett, P, Brooks, T, Brown, K, Chantratita, N, Cheng, A, Dance, D, Decuypere, S, Defenbaugh, D, Gee, J, Houghton, R, Jorakate, P, Lertmemongkolchai, G, Limmathurotsakul, D, Merlin, T, Mukhopadhyay, C, Norton, R, Peacock, S, Rolim, D, Simpson, A, Steinmetz, I, Stoddard, R, Stokes, M, Sue, D, Tuanyok, A, Whistler, T, Wuthiekanun, V, Walke, H

“…There is broad interest in improving detection and diagnosis of this disease not only in melioidosis-endemic regions but also outside these regions because melioidosis may be underreported and poses a potential bioterrorism challenge for public health authorities. …”

Melioidosis in Thailand: present and future by Hinjoy, S, Hantrakun, V, Kongyu, S, Kaewrakmuk, J, Wangrangsimakul, T, Jitsuronk, S, Saengchun, W, Bhengsri, S, Akarachotpong, T, Thamthitiwat, S, Sangwichian, O, Anunnatsiri, S, Sermswan, R, Lertmemongkolchai, G, Tharinjaroen, C, Preechasuth, K, Udpaun, R, Chuensombut, P, Waranyasirikul, N, Anudit, C, Narenpitak, S, Jutrakul, Y, Teparrukkul, P, Teerawattanasook, N, Thanvisej, K, Suphan, A, Sukbut, P, Ploddi, K, Sirichotirat, P, Chiewchanyon, B, Rukseree, K, Hongsuwan, M, Wongsuwan, G, Sunthornsut, P, Wuthiekanun, V, Sachaphimukh, S, Wannapinij, P, Chierakul, W, Chewapreecha, C, Thaipadungpanit, J, Chantratita, N, Korbsrisate, S, Taunyok, A, Dunachie, S, Palittapongarnpim, P, Sirisinha, S, Kitphati, R, Iamsirithaworn, S, Chaowagul, W, Chetchotisak, P, Whistler, T, Wongratanacheewin, S, Limmathurotsakul, D

“…The mortality rate is around 35%. Melioidosis is endemic throughout Thailand, but it is still not uncommon that microbiological facilities misidentify Burkholderia pseudomallei as a contaminant or another organism. …”

Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand. by Cheng, A, Peacock, S, Limmathurotsakul, D, Wongsuvan, G, Chierakul, W, Amornchai, P, Getchalarat, N, Chaowagul, W, White, N, Day, N, Wuthiekanun, V

“…A prospective study was performed to compare a rapid immunochromogenic cassette test (ICT) with the indirect haemagglutination assay (IHA) and clinical rules for the diagnosis of melioidosis in an endemic area. The sensitivity and specificity of the IgG ICT was 86% and 47%, and the IgM ICT was 82% and 47%, respectively. …”

Misidentification of Burkholderia pseudomallei as Acinetobacter species in northern Thailand by Greer, R, Wangrangsimakul, T, Amornchai, P, Wuthiekanun, V, Laongnualpanich, A, Dance, D, Limmathurotsakul, D

“…<p><strong>Background</strong> Burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic throughout the tropics.</p> <p><strong>Methods</strong> A study of reported Acinetobacter spp. bacteraemia was performed at Chiang Rai provincial hospital from 2014 to 2015. …”

Burkholderia pseudomallei and melioidosis by Meumann, EM, Limmathurotsakul, D, Dunachie, SJ, Wiersinga, WJ, Currie, BJ

“…Genomic epidemiological investigations have confirmed B. pseudomallei endemicity in newly recognized regions, including the southern United States. …”

Failure of Burkholderia pseudomallei to Grow in an Automated Blood Culture System. by Teerawattanasook, N, Limmathurotsakul, D, Day, N, Wuthiekanun, V

“…This finding suggests that use of the conventional culture method in conjunction with the BacT/Alert system may improve the isolation rate for B. pseudomallei in melioidosis-endemic areas.…”

Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. by Chantratita, N, Tandhavanant, S, Wongsuvan, G, Wuthiekanun, V, Teerawattanasook, N, Day, N, Limmathurotsakul, D, Peacock, S

“…We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic.…”

Human immune responses to melioidosis and cross-reactivity to low-virulence burkholderia species, Thailand1. by Rongkard, P, Kronsteiner, B, Hantrakun, V, Jenjaroen, K, Sumonwiriya, M, Chaichana, P, Chumseng, S, Chantratita, N, Wuthiekanun, V, Fletcher, HA, Teparrukkul, P, Limmathurotsakul, D, Day, NPJ, Dunachie, SJ

“…We evaluated human immune responses to B. pseudomallei, B. thailandensis, and BTCV in melioidosis patients and healthy persons in B. pseudomallei–endemic areas using a range of humoral and cellular immune assays. …”

Trimethoprim/sulfamethoxazole resistance in clinical isolates of Burkholderia pseudomallei. by Wuthiekanun, V, Cheng, A, Chierakul, W, Amornchai, P, Limmathurotsakul, D, Chaowagul, W, Simpson, A, Short, J, Wongsuvan, G, Maharjan, B, White, N, Peacock, S

“…Antimicrobial susceptibility testing using the disc diffusion method is commonly used in melioidosis-endemic areas, but may overestimate resistance to trimethoprim/sulfamethoxazole. …”

Genetic variation associated with infection and the environment in the accidental pathogen Burkholderia pseudomallei by Chewapreecha, C, Mather, AE, Harris, SR, Hunt, M, Holden, MTG, Chaichana, C, Wuthiekanun, V, Dougan, G, Day, NPJ, Limmathurotsakul, D, Parkhill, J, Peacock, SJ

“…The environmental bacterium Burkholderia pseudomallei causes melioidosis, an important endemic human disease in tropical and sub-tropical countries. …”

Genetic variation associated with infection and the environment in the accidental pathogen Burkholderia pseudomallei by Chewapreecha, C, Mather, AE, Harris, SR, Hunt, M, Holden, MTG, Chaichana, C, Wuthiekanun, V, Dougan, G, Day, NPJ, Limmathurotsakul, D, Parkhill, J, Peacock, SJ

“…The environmental bacterium Burkholderia pseudomallei causes melioidosis, an important endemic human disease in tropical and sub-tropical countries. …”

Accuracy of a commercial IgM ELISA for the diagnosis of human leptospirosis in Thailand. by Desakorn, V, Wuthiekanun, V, Thanachartwet, V, Sahassananda, D, Chierakul, W, Apiwattanaporn, A, Day, N, Limmathurotsakul, D, Peacock, S

“…The Leptospira immunoglobulin M enzyme-linked immunosorbent assay (IgM ELISA) has been recommended for the rapid diagnosis of leptospirosis in endemic areas. We conducted a retrospective case-control study of 218 patients (109 leptospirosis cases confirmed by Leptospira culture and/or microscopic agglutination test and 109 control patients with acute febrile illness) to evaluate the diagnostic accuracy of a commercial IgM ELISA (Panbio) in northeast Thailand. …”