Salivary levels of five microorganisms of root caries in nursing home elderly: a preliminary investigation by Lin Chen, Yuandong Qin, Yuhong Lin, Minquan Du, Yuhong Li, Mingwen Fan

Subjects: “…Quantitative real-time polymerase chain reaction…”
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Transcriptome Analysis and Postprandial Expression of Amino Acid Transporter Genes in the Fast Muscles and Gut of Chinese Perch (Siniperca chuatsi). by Ping Wu, Yulong Li, Jia Cheng, Lin Chen, Ming Zeng, Yuanan Wu, Jianhua Wang, Jianshe Zhang, Wuying Chu

“…Expression of eight amino acid transporter genes was assayed in the fast muscles through quantitative real-time polymerase chain reaction at 0, 1, 3, 6, 12, 24, 48, and 96 h. …”
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LINC01128 - miR-16 interaction regulates the migration and invasion of human chorionic trophoblast cells by Xinyuan Zhao, Fei Liu, Jin Zhang, Jianhua Zhang, Ludan Zhang, Lin Chen

“…Methods: The mRNA levels of LINC01128 and miR-16 in placental tissues and HTR-8/SVneo cells were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Cell Counting Kit (CCK)-8, wound healing assay and transwell assay were used to detect proliferation, migration and invasion. …”
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A prenatal case misunderstood as specimen confusion: 46,XY/46,XY chimerism by Lin Chen, Li Wang, Yang Zeng, Daishu Yin, Feng Tang, Dan Xie, Hongmei Zhu, Lingping Li, Jing Wang

“…Here, we report a case in which the fetus was identified as having tetragametic chimerism based on short tandem repeat - polymerase chain reaction analysis of the family observed during amniocentesis for advanced maternal age. …”
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Deciphering the contributions of cuproptosis in the development of hypertrophic scar using single-cell analysis and machine learning techniques by Binyu Song, Wei Liu, Yuhan Zhu, Yixuan Peng, Zhiwei Cui, Botao Gao, Lin Chen, Zhou Yu, Baoqiang Song

“…Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to confirm the mRNA expression of ATP7A, ULK1, and MTF1 in both HS and normal skin (NS) tissues. …”
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Long noncoding RNA regulates tumor cell proliferation and invasion by epithelial–mesenchymal transition in gastric cancer by Kecheng Zhang, Canrong Lu, Xiaohui Huang, Jianxin Cui, Jiyang Li, Yunhe Gao, Wenquan Liang, Yi Liu, Yang Sun, Hanxuan Liu, Bo Wei, Lin Chen

“…Quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence were used to investigate the potential mechanisms. …”
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Association of Gene Polymorphisms in CXC Chemokine Receptor 5 with Rheumatoid Arthritis Susceptibility by Zhenzhen Su, Lin Chen, Qian Niu, Bin Yang, Zhuochun Huang

“…This case-control study enrolled 285 RA patients and 291 healthy controls. The polymerase chain reaction-ligase detection reaction method was used to genotype rs630923, rs497916, rs3922, and rs676925 in the CXCR5 gene. …”
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Expression and function of myelin expression factor 2 in hepatocellular carcinoma by Peng Zhang, Jiang-Hua Zhao, Lin Chen, Zhao-Lian Bian, Lin-Ling Ju, Hui-Xuan Wang, Wei-Hua Cai

“…Fluorescent quantitative polymerase chain reaction (qPCR) and immunohistochemistry were used to detect the expressions of the MYEF2 mRNA and protein in HCC tissues and cell lines. qPCR and Western blotting were used to validate the efficiency of MYEF2 knockout and overexpression in HCC cells. …”
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Construction of a ceRNA regulatory network to explore potential pathogenesis mechanisms involved in human hepatocellular carcinoma by Yicun Liu, Zhixing Dong, WeiJie Chen, Lin Chen, Linling Ju, Weihua Cai, Xi Luo, Zhaolian Bian

“…The top ten upregulated circRNAs were selected and validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in another 34 HCC patients. …”
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Development and validation of a prognostic model for mitophagy-related genes in colon adenocarcinoma: A study based on TCGA and GEO databases by Yong Tang, Haiyang Guo, Lin Chen, Xiaobo Wang, Qi Chen, Lei Gou, Xiuyu Liu, Xianfei Wang

“…Finally, qualitative reverse transcription polymerase chain reaction and western blotting were performed to assess the expression of prognosis-related MRGs. …”
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Development and validation of a prognostic model for mitophagy-related genes in colon adenocarcinoma: A study based on TCGA and GEO databases. by Yong Tang, Haiyang Guo, Lin Chen, Xiaobo Wang, Qi Chen, Lei Gou, Xiuyu Liu, Xianfei Wang

“…Finally, qualitative reverse transcription polymerase chain reaction and western blotting were performed to assess the expression of prognosis-related MRGs.…”
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Identification and validation of immune-related genes in osteoarthritic synovial fibroblasts by Yaduan Dai, Lin Chen, Zhan Zhang, Xueyong Liu

“…The results were validated by western blotting (WB) and real-time quantitative polymerase chain reaction (qPCR) of clinical synovial samples. …”
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Oridonin Synergistically Enhances the Pro-Apoptotic Effect of Venetoclax on Acute Myeloid Leukemia Cells by Inhibiting AKT Signaling by Lin Chen, Dongbei Li, Xiaoli Guo, Cheng Cheng, Xudong Wei

“…Western blotting and reverse transcription quantitative polymerase chain reaction (RT-qPCR) techniques were used to validate the findings. …”
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Beneficial Rhizobacterium Bacillus amyloliquefaciens SQR9 Induces Plant Salt Tolerance through Spermidine Production by Lin Chen, Yunpeng Liu, Gengwei Wu, Nan Zhang, Qirong Shen, Ruifu Zhang

“…Using quantitative real-time polymerase chain reaction, this study demonstrated that spermidine produced by SQR9 leads to increased glutamine synthetase and glutathione reductase gene expression, leading to increased levels of GSH, which is critical for scavenging reactive oxygen species. …”
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Genome-wide identification of WD40 transcription factors and their regulation of the MYB-bHLH-WD40 (MBW) complex related to anthocyanin synthesis in Qingke (Hordeum vulgare L. var.... by Lin Chen, Yongmei Cui, Youhua Yao, Likun An, Yixiong Bai, Xin Li, Xiaohua Yao, Kunlun Wu

“…Based on the identification and analysis of the barley WD40 family and the transcriptome sequencing (RNA-seq) results, we found that HvWD40-140 (WD40 family; Gene ID: r1G058730), HvANT1 (MYB family; Gene ID: HORVU7Hr1G034630), and HvANT2 (bHLH family; Gene ID: HORVU2Hr1G096810) were important components of the MBW complex related to anthocyanin biosynthesis in Qingke, which was verified via quantitative real-time fluorescence polymerase chain reaction (qRT-PCR), subcellular location, yeast two-hybrid (Y2H), and bimolecular fluorescent complimentary (BiFC) and dual-luciferase assay analyses. …”
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Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3 by Liu-Xia Yuan, Mei Luo, Ruo-Yu Liu, Hui-Xuan Wang, Lin-Ling Ju, Feng Wang, Ya-Li Cao, Zhong-Cheng Wang, Lin Chen

“…Methods Quantitative reverse transcription-polymerase chain reaction(qRT-PCR) was used to measure the expression level of hsa_circ_0005397 and EIF4A3 from paired HCC tissues and cell lines. …”
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Rapid and portable bunyavirus SFTSV RNA testing utilizing catalytic hairpin assembly coupled with lateral flow immunoassay by Lin Chen, Mengyin Ma, Mingyuan Zou, Liwei Zhao, Mingrong Ou, Yu Geng, Chuang Li, Han Shen, Yuxin Chen

“…Currently, reverse transcription-polymerase chain reaction is the gold standard for diagnosing SFTS virus (SFTSV) infection, which requires sophisticated equipment and professional personnel that are frequently unavailable in most SFTS endemic rural areas. …”
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OTUD1 ameliorates cerebral ischemic injury through inhibiting inflammation by disrupting K63-linked deubiquitination of RIP2 by Shengnan Zheng, Yiquan Li, Xiaomeng Song, Mengting Wu, Lu Yu, Gan Huang, Tengfei Liu, Lei Zhang, Mingmei Shang, Qingfen Zhu, Chengjiang Gao, Lin Chen, Huiqing Liu

“…The change of IL-1β, IL-6 and TNF-α was detected by ELISA assay and quantitative real-time polymerase chain reaction. Immunoprecipitation and confocal microscopy were used to study the interaction of RIP2 and OTUD1. …”
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Identification of clinical prognostic features of esophageal cancer based on m6A regulators by Huimei Wang, Yiping Zhang, Lin Chen, Yufeng Liu, Chen Xu, Dongxian Jiang, Qi Song, Haixing Wang, Liyan Wang, Yu Lin, Yuanmei Chen, Junqiang Chen, Yuanji Xu, Yingyong Hou

“…The m6A expression of key genes in clinical specimens was validated using quantitative reverse transcription polymerase chain reaction (qRT-PCR).ResultsIn ESCA tissues, most of the 23 regulators were significantly differentially expressed. …”
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PIWIL1 interacting RNA piR-017724 inhibits proliferation, invasion, and migration, and inhibits the development of HCC by silencing PLIN3 by Yi-Jing Wu, Jie Wang, Peng Zhang, Liu-Xia Yuan, Lin-Ling Ju, Hui-Xuan Wang, Lin Chen, Ya-Li Cao, Wei-Hua Cai, Yi Ni, Min Li

“…Studies on the role and functional mechanism of piRNAs in HCC development and progression are limited.MethodsQuantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to detect the expression of piR-017724 in both HCC tissues and cells. …”
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