Glutathione Plays a Positive Role in the Proliferation of <i>Pinus koraiensis</i> Embryogenic Cells by Fang Gao, Chunxue Peng, Yue Zhang, Hao Wang, Hailong Shen, Ling Yang

“…To reveal the regulatory mechanism of proliferation in woody plant cell lines with different proliferative potential, we used Korean pine cell lines with high proliferative potential 001#–001 (Fast) and low proliferative potential 001#–010 (Slow) for analysis. …”
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Improved Method for Cryopreservation of Embryogenic Callus of <i>Fraxinus mandshurica</i> Pupr. by Vitrification by Xueqing Liu, Yingying Liu, Xiaoqian Yu, Iraida Nikolaevna Tretyakova, Alexander Mikhaylovich Nosov, Hailong Shen, Ling Yang

“…The best pre-culture method was to pre-culture EC on 0.5 mol·L⁻¹ sucrose medium for 3 d, load and culture in the liquid woody plant medium (WPM) supplemented with 2 mol·L⁻¹ glycerol and 0.4 mol·L⁻¹ sucrose for 60 min, then dehydrate in 2 mL of plant vitrification solution 2 (PVS2) (30% glycerol + 15% dimethyl sulfoxide (DMSO) + 15% ethylene glycol + 0.4 mol·L⁻¹ sucrose + liquid WPM). …”
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Cryopreservation of <i>Fraxinus mandshurica</i> Rupr. by Using the Slow Cooling Method by Xiaoqian Yu, Yingying Liu, Xueqing Liu, Iraida Nikolaevna Tretyakova, Alexander Mikhaylovich Nosov, Hailong Shen, Ling Yang

“…During thawing, the cryopreservation tube was taken out from liquid nitrogen, directly placed in a water bath at 40 °C for 2 min, and used for culturing on the woody plant media + 0.1 mg·L⁻¹ 6-benzyladenine + 0.15 mg·L⁻¹ 2, 4-dichlorophenoxyacetic acid. …”
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Optimization of Key Technologies for Induction of Embryogenic Callus and Maturation of Somatic Embryos in Korean Pine (<i>Pinus koraiensis</i>) by Fang Gao, Yujie Shi, Ruirui Wang, Iraida Nikolaevna Tretyakova, Alexander Mikhaylovich Nosov, Hailong Shen, Ling Yang

“…Somatic embryogenesis (SE), which leads to the formation of embryonic callus (EC) tissue, is the most promising method for large-scale production and selective breeding of woody plants. However, in many species, SE suffers from low induction and proliferation rates, hindering the production of improved plant materials. …”
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Exogenous Glutathione Promotes the Proliferation of <i>Pinus koraiensis</i> Embryonic Cells and the Synthesis of Glutathione and Ascorbic Acid by Fang Gao, Yujie Shi, Ruirui Wang, Iraida Nikolaevna Tretyakova, Alexander Mikhaylovich Nosov, Hailong Shen, Ling Yang

“…Somatic embryogenesis (SE), which leads to the formation of embryonic callus (EC) tissue, is the most promising method for large-scale production and selective breeding of woody plants. However, in many species, SE suffers from low proliferation rates, hindering the production of improved plant materials. …”
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