Sperm DNA damage compromises embryo development, but not oocyte fertilisation in pigs by Yentel Mateo-Otero, Marc Llavanera, Sandra Recuero, Ariadna Delgado-Bermúdez, Isabel Barranco, Jordi Ribas-Maynou, Marc Yeste

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Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model by Yentel Mateo-Otero, Francisco Madrid-Gambin, Marc Llavanera, Alex Gomez-Gomez, Noemí Haro, Oscar J. Pozo, Marc Yeste

“…Metabolic activity of mammalian sperm is shown to influence reproductive outcomes beyond fertilisation.…”
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Embryo development is impaired by sperm mitochondrial-derived ROS by Yentel Mateo-Otero, Marc Llavanera, Marc Torres-Garrido, Marc Yeste

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Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs by Marc Llavanera, Yentel Mateo-Otero, Estel Viñolas-Vergés, Sergi Bonet, Marc Yeste

“…Interestingly, samples with lower mtDNAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater mtDNAc. …”
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Glutathione S-Transferases Play a Crucial Role in Mitochondrial Function, Plasma Membrane Stability and Oxidative Regulation of Mammalian Sperm by Marc Llavanera, Ariadna Delgado-Bermúdez, Samuel Olives, Yentel Mateo-Otero, Sandra Recuero, Sergi Bonet, Beatriz Fernández-Fuertes, Marc Yeste, Isabel Barranco

“…Glutathione S-transferases (GSTs) are essential sperm antioxidant enzymes involved in cell protection against oxidative stress and toxic chemicals, preserving sperm function and fertilising ability. Artificial insemination (AI) in pigs is commonly carried out through the use of liquid-stored semen at 17 °C, which not only reduces sperm metabolic activity but also sperm quality and AI-farrowing rates within the 72 h of storage. …”
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Animal board invited review: An update on the methods for semen quality evaluation in swine – from farm to the lab by Carolina Maside, Sandra Recuero, Albert Salas-Huetos, Jordi Ribas-Maynou, Marc Yeste

“…Furthermore, sperm chromatin condensation and DNA integrity, despite not being routinely assessed, may also help determine the causes of reduced fertilising capacity. Sperm DNA integrity can be evaluated through direct (Comet, transferase deoxynucleotide nick end labelling (TUNEL) and its in situ nick variant) or indirect tests (Sperm Chromatin Structure Assay, Sperm Chromatin Dispersion Test), whereas chromatin condensation can be determined with Chromomycin A3. …”
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