Spinyhead Croaker Germ Cells Gene <i>dnd</i> Visualizes Primordial Germ Cells in Medaka by Cong Xu, Yu Li, Zhengshun Wen, Muhammad Jawad, Lang Gui, Mingyou Li

“…Researches on the development of primordial germ cell (PGC) and reproduction biology were an emergency for the long-term conservation of the involved species. …”
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Evolutionarily conserved boule and dazl identify germ cells of Coilia nasus by Xiao Chen, Peng Song, Jiao Xia, Jing Guo, Yonghai Shi, Ying Zhong, Mingyou Li

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Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout. by Mingyou Li, Qian Shen, Hongyan Xu, Foong Mei Wong, Jianzhou Cui, Zhendong Li, Ni Hong, Li Wang, Haobin Zhao, Bo Ma, Yunhan Hong

“…BACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. …”
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Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro by Xiao Chen, Yuting Kan, Ying Zhong, Muhammad Jawad, Wenbo Wei, Kaiyan Gu, Lang Gui, Mingyou Li

“…Our research helps to explore the potential and regulation mechanism of fish SSC differentiation and spermatogenesis in vitro, provides a new way for solving the problem of fish genetic resource degradation and lays a foundation for further research on fish germ cell transplantation.…”
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Establishment of a <i>Coilia nasus</i> Gonadal Somatic Cell Line Capable of Sperm Induction <i>In Vitro</i> by Yuting Kan, Ying Zhong, Muhammad Jawad, Xiao Chen, Dong Liu, Mingchun Ren, Gangchun Xu, Lang Gui, Mingyou Li

“…RT-PCR results revealed that the CnGSC expressed some somatic cell markers such as <i>clu</i>, <i>fshr</i>, <i>hsd3β,</i> and <i>sox9b</i> instead of germ cell markers like <i>dazl</i>, <i>piwi,</i> and <i>vasa</i>. …”
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Characterization and expression analysis of sox3 in medaka gonads by Quan Pu, Yuan Ma, Ying Zhong, Jing Guo, Lang Gui, Mingyou Li

“…In this study, medaka sox3 was isolated and its expression patterns were compared with germ cell gene vasa in adult gonads. By reverse transcription-polymerase chain reaction (RT-PCR) analysis, sox3 RNA expression was detected in the brain, eyes and gonads. …”
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Characterization and expression analysis of gonad specific igf3 in the medaka ovary by Jiale Xie, Ying Zhong, Yuli Zhao, Wenjie Xie, Jing Guo, Lang Gui, Mingyou Li

“…Collectively, we demonstrated that gonad specific igf3 could mark medaka ovarian somatic cells and germ cells. These findings highlight the importance of igf3 during ovarian development.…”
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Medaka gcnf is a component of chromatoid body during spermiogenesis by Zinan Xie, Peng Song, Ying Zhong, Jing Guo, Lang Gui, Mingyou Li

“…Germ cells of many organisms exhibit a unique perinuclear cytoplasmic cloud-like structure called the chromatoid body (CB). …”
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Differential Expression of Duplicate Insulin-like Growth Factor-1 Receptors (<i>igf1rs</i>) in Medaka Gonads by Wenbo Wei, Yefei Zhu, Cancan Yuan, Yuli Zhao, Wenzong Zhou, Mingyou Li

“…Collectively, we demonstrated that differential <i>igf1rs</i> RNA expression identifies medaka meiotic germ cells and somatic cells of both sexes. These findings highlight the importance of the <i>igf</i> system in the development of fish gonads.…”
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