Rapid characterisation of hERG channel kinetics II: temperature dependence by Lei, CL, Clerx, M, Beattie, KA, Melgari, D, Hancox, JC, Gavaghan, DJ, Polonchuk, L, Wang, K, Mirams, GR

“…In this Part II, we use this protocol to study the temperature dependence of hERG kinetics using Chinese hamster ovary cells overexpressing hERG1a on the Nanion SyncroPatch 384PE, a 384-well automated patch-clamp platform, with temperature control. …”

Rapid characterisation of hERG channel kinetics I: using an automated high-throughput system by Lei, CL, Clerx, M, Gavaghan, D, Polonchuk, L, Mirams, GR, Wang, K

“…We demonstrate its use on the Nanion SyncroPatch 384PE, a 384-well automated patch-clamp platform, by applying it to Chinese hamster ovary cells stably expressing hERG1a. From these recordings, we construct 124 cell-specific variants/parameterizations of a hERG model at 25°C. …”

Sinusoidal voltage protocols for rapid characterisation of ion channel kinetics by Beattie, KA, Hill, AP, Bardenet, R, Cui, Y, Vandenberg, JI, Gavaghan, DJ, de Boer, TP, Mirams, GR

“…We present a new method for rapidly exploring and characterising ion channel kinetics, applying it to the hERG potassium channel as an example, with the aim of generating a quantitatively predictive representation of the ion current. …”

Accounting for variability in ion current recordings using a mathematical model of artefacts in voltage-clamp experiments by Lei, CL, Clerx, M, Whittaker, D, Gavaghan, DJ, de Boer, TP, Mirams, GR

“…In a previous study, we fitted cell-specific models to hERG1a (Kv11.1) recordings simultaneously measured using an automated high-throughput system, and studied cell-cell variability by inspecting the resulting model parameters. …”