Field-based flow cytometry for ex vivo characterization of Plasmodium vivax and P. falciparum antimalarial sensitivity. by Russell, B, Malleret, B, Suwanarusk, R, Anthony, C, Kanlaya, S, Lau, Y, Woodrow, C, Nosten, F, Renia, L

“…Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. …”

Defining the in vivo phenotype of artemisinin-resistant falciparum malaria: a modelling approach by White, L, Flegg, J, Phyo, A, Wiladpai-ngern, J, Bethell, D, Plowe, C, Anderson, T, Nkhoma, S, Nair, S, Tripura, R, Stepniewska, K, Pan-Ngum, W, Silamut, K, Cooper, B, Lubell, Y, Ashley, E, Nguon, C, Nosten, F, White, N, Dondorp, A

“…The findings are important for the interpretation of in vitro sensitivity tests and molecular markers for artemisinin resistance and for contextualising the 'day 3' threshold to account for initial parasitaemia and sample size.…”

A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border. by Watthanaworawit, W, Turner, P, Turner, C, Tanganuchitcharnchai, A, Jarman, R, Blacksell, S, Nosten, F

“…We prospectively evaluated the performance of real-time reverse transcriptase (rRT)-PCR, NS-1 antigen and IgM antibody tests to confirm dengue virus infection in acute blood specimens from 162 patients presenting with undifferentiated febrile illness compatible with dengue infection. rRT-PCR was the most sensitive test (89%) and potentially could be used as a single test for confirmation of dengue infection. …”

A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border by Watthanaworawit, W, Turner, P, Turner, C, Tanganuchitcharnchai, A, Jarman, R, Blacksell, S, Nosten, F

“…We prospectively evaluated the performance of real-time reverse transcriptase (rRT)-PCR, NS-1 antigen and IgM antibody tests to confirm dengue virus infection in acute blood specimens from 162 patients presenting with undifferentiated febrile illness compatible with dengue infection. rRT-PCR was the most sensitive test (89%) and potentially could be used as a single test for confirmation of dengue infection. …”

Reverse transcription PCR to detect low density malaria infections by Christensen, P, Bozdech, Z, Watthanaworawit, W, Renia, L, Malleret, B, Ling, C, Nosten, F

“…<br><strong>Background: </strong>Targeted malaria elimination strategies require highly sensitive tests to detect low density malaria infections (LDMI). …”

Artemisinin resistance in Plasmodium falciparum malaria. by Dondorp, A, Nosten, F, Yi, P, Das, D, Phyo, A, Tarning, J, Lwin, K, Ariey, F, Hanpithakpong, W, Lee, S, Ringwald, P, Silamut, K, Imwong, M, Chotivanich, K, Lim, P, Herdman, T, An, S, Yeung, S, Singhasivanon, P, Day, N, Lindegardh, N, Socheat, D, White, N

“…These markedly different parasitologic responses were not explained by differences in age, artesunate or dihydroartemisinin pharmacokinetics, results of isotopic in vitro sensitivity tests, or putative molecular correlates of P. falciparum drug resistance (mutations or amplifications of the gene encoding a multidrug resistance protein [PfMDR1] or mutations in the gene encoding sarco-endoplasmic reticulum calcium ATPase6 [PfSERCA]). …”