Molecular analysis of isolates of Salmonella typhi obtained from patients with fatal and nonfatal typhoid fever. by Thong, Kwai Lin, Passey, M., Clegg, A., Combs, B.G., Yassin, R.M., Pang, T.

“…Of the 52 isolates tested, 11 were obtained from patients with fatal typhoid fever and 41 were obtained from patients with nonfatal disease. …”

Molecular analysis of isolates of Salmonella typhi obtained from fatal, mild and sporadic cases of typhoid fever by Thong, K.L., Megan, Passey, Alison, Clegg, barry, G.combs, Rohani, M.Y., Pang, T.

“…Of the 52 isolates tested, 11 were obtained from patients with fatal typhoid fever and 41 were obtained from patients with nonfatal disease. …”
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Induction and characterization of heat shock proteins of Salmonella typhi and their reactivity with sera from patients with typhoid fever by Tang, S.W., AbuBakar, Sazaly, Devi, S., Puthucheary, S., Pang, T.

“…Immunoblotting experiments with sera from patients with a culture-positive diagnosis of typhoid fever showed the presence of antibodies to these HSPs. …”

Epidemiologic analysis of sporadic Salmonella typhi isolates and those from outbreaks by pulsed-field gel electrophoresis. by Thong, Kwai Lin, Cheong, Y.M., Puthucheary, S., Koh, C.L., Pang, T.

“…Pulsed-field gel electrophoresis (PFGE) was used to compare and analyze 158 isolates of Salmonella typhi from five well-defined outbreaks of typhoid fever in Malaysia and also isolates involved in sporadic cases of typhoid fever occurring during the same period. …”

A probable case of laboratory-acquired infection with salmonella typhi: evidence from phage typing, antibiograms, and analysis by pulsed-field gel electrophoresis by Thong, Kwai Lin, Cheong, Y.M., Pang, T.

“…Objective: To report a probable case of laboratory-acquired typhoid fever involving a female laboratory technologist at a major diagnostic bacteriology laboratory in Kuala Lumpur, Malaysia. …”
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Genetic dynamics and molecular epidemiology of Salmonella typhi by Thong, Kwai Lin, Nair, S., Subramaniam, G., Puthucheary, S.D., Yassin, R.M., Cheong, Y.M., Liu, S.L., Sanderson, K.E., Cordano, A.M., Pang, T.

“…A large number of S. typhi isolates from various geographic regions, representing sporadic, outbreak, and environmental isolates, as well as isolates from fatal and non-fatal cases of typhoid fever have been analysed by pulsed-field gel electrophoresis (PFGE) with restriction endonucleases XbaI, SpeI, AvrII, and CeuI. …”
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In vivo genetic stability of Salmonella typhi detected by ribotyping and pulsed field gel electrophoresis by Thong, Kwai Lin, Puthucheary, S.D., Pang, T.

“…Ribotyping, PCR-ribotyping and pulsed-field gel electrophoresis were used to analyze five serial isolates of Salmonella typhi obtained from a single patient with relapsing typhoid fever over a 7-week period. The 5 isolates were genetically identical by all three methods thus suggesting relapsing infection with the same strain and implying in vivo genomic stability of S. typhi even in the presence of selective pressures of antibiotic treatment and host defenses.…”
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Analysis of Salmonella typhi isolates from Southeast Asia by pulsed-field gel electrophoresis. by Thong, Kwai Lin, Puthucheary, S., Yassin, R.M., Sudarmono, P., Padmidewi, M., Soewandojo, E., Handojo, I., Sarasombath, S., Pang, T.

“…Pulsed-field gel electrophoresis (PFGE) revealed that multiple genetic variants of Salmonella typhi are simultaneously present in Southeast Asia and are associated with sporadic cases of typhoid fever and occasional outbreaks. Comparative analysis of PFGE patterns also suggested that considerable genetic diversity exists among S. typhi strains and that some PFGE patterns are shared between isolates obtained from Malaysia, Indonesia, and Thailand, implying movement of these strains within these regions of Southeast Asia, where they are endemic.…”

Comparative analysis of salmonella typhi by rRNA gene restriction and phage typing by Thong, Kwai Lin, Altwegg, M., Pang, T.

“…Phage typing and ribotyping were used to analyze 19 isolates of Salmonella typhi from sporadic cases and two outbreaks of typhoid fever in Malaysia in 1987 and 1990. The two outbreaks were associated with phage types D1 and E1 in Penang and Alor Setar respectively, and phage types D1, E1 and A (with 3 untypeable isolates) were present among the sporadic cases. …”

Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. by Lachumanan, R., Devi, S., Cheong, Y.M., Rodda, S.J., Pang, T.

“…Further analysis of the specificity of peptide binding with five of these peptides indicated that the peptides showed significantly stronger binding to scrub typhus patients' sera than they did to sera from patients with other febrile illnesses common in the region, i.e., malaria, dengue fever, typhoid fever, and leptospirosis. The main antibody class binding to these peptides appears to be immunoglobulin M, and there appears to be little correlation between reactivity with peptides and antibody titers measured by the indirect immunoperoxidase test.…”

Identification of antigenic epitopes of S. typhi using phage display epitopes library by Thong, Kwai Lin, Subramaniam, G., Devi, S., Puthucheary, S.D., Yu, M., Wang, L.F., Pang, T.

“…Utilizing a biopanning assay, where diluted sera from patients with typhoid fever were immobilized on a solid support (paramagnetic beads), antigenic epitope from the genome-targeted phage library were identified following binding and subsequent elution of recombinant phages and DNA sequencing of relevant inserts. …”
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Multidrug-Resistant Strains of Salmonella enterica serotype typhi are Genetically Homogenous and Coexist with Antibiotic-Sensitive Strains as Distinct, Independent Clones by Thong, Kwai Lin, Bhutta, Z.A., Pang, T.

“…Methods: One hundred isolates of S. typhi in humans (50 MDR and 50 antibiotic-sensitive isolates) from sporadic cases of typhoid fever were analyzed by Vi-phage typing, antibiograms and PFGE. …”
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Molecular analysis of environmental and human isolates of Salmonella typhi. by Thong, Kwai Lin, Cordano, A.M., Yassin, R.M., Pang, T.

“…Thirteen of the 54 isolates were obtained from environmental sources (sewage and river water), and the rest were isolates from clinical cases of typhoid fever. Considerable genetic diversity was detected among the human isolates obtained in 1994, as evidenced by the presence of 14 to 19 different PFGE patterns among 20 human isolates, with F (coefficient of similarity) values ranging from 0.69 to 1.0 (XbaI), 0.61 to 1.0 (AvrII), and 0.70 to 1.0 (SpeI). …”

A simple adherence test for detection of IgM antibodies in typhoid by Ong, L.Y., Pang, T., Lim, S.H., Tan, E.L., Puthucheary, S.D.

“…A total of 82 sera from non-typhoidal fevers (leptospirosis, typhus, dengue fever) showed no reactivity in this test. …”