The mechanism of action and physiological role of PLCζ at fertilisation by Hachem, A

“…</p> <p>Lastly, oocytes fertilised in vivo and in vitro by PLCζ-null sperm were found to be polyspermic. …”

New insights into the mechanism of egg activation in fish by Coward, K, Parrington, J

“…The commercial culture of several important species of fish has long been associated with productivity problems generally attributable to fertilisation rates, hatching rates, and embryonic development. …”

Mechanisms of oocyte activation in the presence and absence of PLCζ by Sahu, RK

“…PLCζ RNA and protein microinjection and knockout studies in a mouse model have confirmed its primary role in releasing calcium (Ca2+) in the oocyte at fertilisation. Studies revealed that a few delayed Ca2+ transients can still occur in the oocyte after fertilisation with PLCζ knockout sperm, resulting in a reduced embryo formation rate and increased polyspermy, but otherwise apparently normal development to term, indicating the presence of a secondary factor(s) which can stimulate Ca2+ release in the oocyte. …”

PLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo development by Saunders, C, Larman, MG, Parrington, J, Cox, L, Royse, J, Blayney, L, Swann, K, Lai, F

“…Upon fertilisation by sperm, mammalian eggs are activated by a series of intracellular Ca2+ oscillations that are essential for embryo development. …”

PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development. by Saunders, C, Larman, MG, Parrington, J, Cox, L, Royse, J, Blayney, L, Swann, K, Lai, F

“…Upon fertilisation by sperm, mammalian eggs are activated by a series of intracellular Ca(2+) oscillations that are essential for embryo development. …”

In vivo gene transfer into the testis by electroporation and viral infection--a novel way to study testis and sperm function. by Parrington, J, Coward, K, Hibbitt, O, Kubota, H, Young, C, McIlhinney, J, Jones, O

“…The study of gene function in testis and sperm has been greatly assisted by creation of transgenic mice by injection of a transgene into the fertilised egg. However this approach is costly and laborious and is not applicable to other species of importance for the study of sperm function, such as the hamster. …”