CircRAPGEF5 interacts with RBFOX2 to confer ferroptosis resistance by modulating alternative splicing of TFRC in endometrial cancer by Jun Zhang, Shuaijun Chen, Sitian Wei, Shuangshuang Cheng, Rui Shi, Rong Zhao, Wei Zhang, Qi Zhang, Teng Hua, Dilu Feng, Zhicheng Yu, Hongbo Wang

“…Additionally, a series of gain- and loss-of-function experiments demonstrated that knocking down or overexpressing RBFOX2 reversed the effects of knocking down or overexpressing circRAPGEF5 in EC cells. …”
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Current status of xenotransplantation research and the strategies for preventing xenograft rejection by Qiao Zhou, Qiao Zhou, Qiao Zhou, Ting Li, Kaiwen Wang, Qi Zhang, Zhuowen Geng, Shaoping Deng, Shaoping Deng, Shaoping Deng, Chunming Cheng, Yi Wang

“…In this review, we comprehensively discussed the mechanism of these immunological rejections and summarized the strategies for preventing them, such as generation of gene knock out donors by different genome editing tools and the use of immunosuppressive regimens. …”
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Involvement of <i>Laccase2</i> in Cuticle Sclerotization of the Whitefly, <i>Bemisia tabaci</i> Middle East–Asia Minor 1 by Chun-Hong Yang, Qi Zhang, Wan-Qing Zhu, Yan Shi, He-He Cao, Lei Guo, Dong Chu, Zhaozhi Lu, Tong-Xian Liu

“…This result demonstrates that <i>BtLac2</i> plays an important role in the cuticle hardening of <i>B. tabaci</i> and suggests a potential management strategy using RNAi to knock down <i>BtLac2</i> expression.…”
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Carotenoid biosynthesis is associated with low-temperature adaptation in Rhodosporidium kratochvilovae by Rui Guo, Tao Liu, Caina Guo, Gongshui Chen, Jingdie Fan, Qi Zhang

“…Results Carotenoid biosynthesis in YM25235 was inhibited by knocking out the bifunctional lycopene cyclase/phytoene synthase gene (RKCrtYB) using the established CRISPR/Cas9 gene-editing system based on endogenous U6 promoters. …”
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CRISPR/Cas9-Mediated Mutagenesis of Sex-Specific <i>Doublesex</i> Splicing Variants Leads to Sterility in <i>Spodoptera frugiperda</i>, a Global Invasive Pest by Junwen Gu, Jingyi Wang, Honglun Bi, Xuehai Li, Austin Merchant, Porui Zhang, Qi Zhang, Xuguo Zhou

“…As expected, abnormal development of both the external and internal genitalia was observed during the pupal and adult stages. Interestingly, knocking out sex-specific <i>dsx</i> variants in <i>S. frugiperda</i> led to significantly reduced fecundity and fertility in adults of corresponding sex. …”
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Deacetylation of TFEB promotes fibrillar Aβ degradation by upregulating lysosomal biogenesis in microglia by Jintao Bao, Liangjun Zheng, Qi Zhang, Xinya Li, Xuefei Zhang, Zeyang Li, Xue Bai, Zhong Zhang, Wei Huo, Xuyang Zhao, Shujiang Shang, Qingsong Wang, Chen Zhang, Jianguo Ji

“…Subsequently, SIRT1 overexpression enhanced lysosomal function and fAβ degradation by upregulating transcriptional levels of TFEB downstream targets, which could be inhibited when TFEB was knocked down. Furthermore, overexpression of deacetylated TFEB at K116R mutant in microglia accelerated intracellular fAβ degradation by stimulating lysosomal biogenesis and greatly reduced the deposited amyloid plaques in the brain slices of APP/PS1 transgenic mice. …”
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EFEMP1 in Direct Inguinal Hernia: correlation with TIMP3 and Regulation Toward Elastin Homoeostasis as Well as Fibroblast Mobility by Xiaohui Peng, Zhongwu Guo, Yinlong Zhang, Baichen Sun, Qi Zhang

“…Fibroblasts L929 cells were transfected with EFEMP1 overexpression plasmid or knock-down plasmid to investigate the influence of EFEMP1 dysregulation on L929 cell migration, invasion, TIMP3, MMP9 and ELN expressions. …”
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Alternative Splicing of lncRNAs From SNHG Family Alters snoRNA Expression and Induces Chemoresistance in HepatoblastomaSummary by Ni Zhen, Jiabei Zhu, Siwei Mao, Qi Zhang, Song Gu, Ji Ma, Yue Zhang, Minzhi Yin, Haojie Li, Nan Huang, Han Wu, Fenyong Sun, Binwu Ying, Lin Zhou, Qiuhui Pan

“…Functionally, PABPN1 acts as an oncofetal splicing regulator in HB by promoting cell proliferation and DNA damage repair via inducing the intron retention of SNHG19. Knock-down of PABPN1 increases the cisplatin sensitivity of HB PDOs. …”
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Selective deletion of zinc transporter 3 in amacrine cells promotes retinal ganglion cell survival and optic nerve regeneration after injury by Zhe Liu, Jingfei Xue, Canying Liu, Jiahui Tang, Siting Wu, Jicheng Lin, Jiaxu Han, Qi Zhang, Caiqing Wu, Haishun Huang, Ling Zhao, Yehong Zhuo, Yiqing Li

“…In this study, we conditionally knocked out zinc transporter 3 (ZnT3) in amacrine cells or retinal ganglion cells to construct two transgenic mouse lines (VGATCreZnT3fl/fl and VGLUT2CreZnT3fl/fl, respectively). …”
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Niclosamide improves cancer immunotherapy by modulating RNA-binding protein HuR-mediated PD-L1 signaling by Qi Zhang, Zhe Yang, Xinbao Hao, Lauren J. Dandreo, Lily He, Yuxia Zhang, Fen Wang, Xiaoqing Wu, Liang Xu

“…Results We found that HuR directly bound to and stabilized PD-L1 mRNA. Knocking out HuR reduced PD-L1 levels and promoted T cell activation. …”
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The novel Nsp9-interacting host factor H2BE promotes PEDV replication by inhibiting endoplasmic reticulum stress-mediated apoptosis by Xingang Xu, Mingrui Ma, Xiaojie Shi, Yuchao Yan, Yi Liu, Naling Yang, Quanqiong Wang, Shuxia Zhang, Qi Zhang

“…Overexpression of H2BE led to significantly inhibited GRP78 expression, phosphorylated PERK (p-PERK), phosphorylated eIF2 (p-eIF2), phosphorylated IRE1 (p-IRE1), and phosphorylated JNK (p-JNK); negatively regulated CHOP and Bax expression and caspase-9 and caspase-3 cleavage; and promoted Bcl-2 production. Knocking down H2BE exerted the opposite effects. Furthermore, we found that after deletion of amino acids 1–28, H2BE did not promote PEDV replication. …”
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Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes by Zhonghao Li, Qi Zhang, Xianyan Liu, Ming Zhao

“…Besides, abolishment or mutation of peroxisome proliferator-activated receptor α (PPARα)/RXR binding site on SR-BI promoter eliminated SR-BI reporter gene luciferase activity even in the presence of the recombinant humanized IgG1 antibody. Knock down the neonatal Fc receptor (FcRn) on hepatocytes impaired the effect of recombinant humanized IgG1 antibody on activation of ERK1/2, as well as upregulation of SR-BI, ApoA-I, and ApoA-II expression. …”
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PEDV inhibits HNRNPA3 expression by miR-218-5p to enhance cellular lipid accumulation and promote viral replication by Xiaojie Shi, Qi Zhang, Naling Yang, Quanqiong Wang, Yanxia Zhang, Xingang Xu

“…We demonstrated that the expression of HNRNPA3 was downregulated by PEDV-induced miR-218-5p through targeting its 3′ untranslated region. Interestingly, knocking down HNRNPA3 facilitated the PEDV replication by promoting cellular lipid synthesis. …”
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LINC00478-derived novel cytoplasmic lncRNA LacRNA stabilizes PHB2 and suppresses breast cancer metastasis via repressing MYC targets by Rong Guo, Yonghui Su, Qi Zhang, Bingqiu Xiu, Sheng Huang, Weiru Chi, Liyi Zhang, Lun Li, Jianjing Hou, Jia Wang, Jiajian Chen, Yayun Chi, Jingyan Xue, Jiong Wu

“…Paired-guide CRISPR/cas9 and CRISPR/dead-Cas9 systems was used to knock out or activate the expression of LacRNA. Cell migration and invasion assay was performed to confirm the phenotype of LacRNA. …”
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