Manipulation of the conformation and enzymatic properties of T1 Lipase by site-directed mutagenesis of the protein core by Abdul Wahab, Roswanira, Basri, Mahiran, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Abdul Rahman, Mohd. Basyaruddin, Leow, Thean Chor

Effects of lid 1 mutagenesis on lid displacement, catalytic performances and thermostability of cold-active Pseudomonas AMS8 lipase in toluene by Yaacob, Norhayati, Ahmad Kamarudin, Nor Hafizah, Leow, Adam Thean Chor, Salleh, Abu Bakar, Raja Abdul Rahman, Raja Noor Zaliha, Mohamad Ali, Mohd Shukuri

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Facile modulation of enantioselectivity of thermophilic Geobacillus zalihae lipase by regulating hydrophobicity of its Q114 oxyanion by Abdul Wahab, Roswanira, Basri, Mahiran, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Abdul Rahman, Mohd Basyaruddin, Leow, Adam Thean Chor

Subjects: “…T1 lipase; Geobacillus zalihae; 2DSN; Site-directed mutagenesis; Enantioselectivity; Oxyanion…”
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Main structural targets for engineering lipase substrate specificity by Albayati, Samah Hashim, Masomian, Malihe, Ishak, Siti Nor Hasmah, Mohamad Ali, Mohd Shukuri, Leow, Adam Thean Chor, Mohd Shariff, Fairolniza, Muhd Noor, Noor Dina, Raja Abdul Rahman, Raja Noor Zaliha

“…This review detailed the main sources, classification, structural properties, and mutagenic approaches, such as rational design (site direct mutagenesis, iterative saturation mutagenesis) and direct evolution (error prone PCR, DNA shuffling), for achieving modification goals. …”
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Purified T1 lipase, a novel construct of T1 lipase and methods thereoF by Raja Abdul Rahman, Raja Noor Zaliha, Saleh, Abu Bakar, Basri, Mahiran, Leow, Adam Thean Chor, Che Hussi, Che Haznie Ayu

“…The present invention relates to method to substitute second step affinity chromatography with ion exchange chromatography by site-directed mutagenesis at downstream region of GST sequences (H215R and G213R). …”

Enzymatic properties and mutational studies of chalcone synthase from Physcomitrella patens by Raja Abdul Rahman, Raja Noor Zaliha, Zakaria, Iffah Izzati, Salleh, Abu Bakar, Basri, Mahiran

“…The Cys-His-Asn catalytic triad found in other plant chalcone synthase predicted polypeptides is conserved in PpCHS. Site directed mutagenesis involving these amino acids residing in the active-site cavity revealed that the cavity volume of the active-site plays a significant role in the selection of starter molecules as well as product formation. …”
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Insight into improved thermostability of cold-adapted staphylococcal lipase by glycine to cysteine mutation by Veno, Jiivittha, Raja Abdul Rahman, Raja Noor Zaliha, Masomian, Malihe, Mohamad Ali, Mohd Shukuri, Ahmad Kamarudin, Nor Hafizah

“…Numerous studies have revealed promising strategies to improve thermostability and random mutagenesis often leads to unexpected yet interesting findings in engineering stability. …”
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Enhancement of a protocol purifying T1 lipase through molecular approach by Che Hussian, Che Haznie Ayu, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri

“…Therefore, a rational design of GST isoelectric point (pI) was implemented by prediction using ExPASy software in order to enhance the differences of pI values between GST and matured T1 lipase. Site-directed mutagenesis at two locations flanking the downstream region of GST sequences (H215R and G213R) was successfully performed. …”
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The role of Arg157Ser in improving the compactness and stability of ARM lipase by Salleh, Abu Bakar, Abd Rahim, Arilla Sri Masayu, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Thean Chor, Basri, Mahiran

“…To validate the computational data, the substitution of Arg157 to Ser has been conducted using site-directed mutagenesis experimentally. The catalytic efficiency (kcat/KM) of R157S lipase was refold better than ARM lipase of 70°C. …”
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Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from Geobacillus zalihae strain T1 by Ruslan, Rudzanna, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Thean Chor, Mohamad Ali, Mohd Shukuri, Basri, Mahiran, Salleh, Abu Bakar

“…Mutant D311E and K344R were constructed using site-directed mutagenesis to introduce an additional ion pair at the inter-loop and the intra-loop, respectively, to determine the effect of ion pairs on the stability of T1 lipase isolated from Geobacillus zalihae. …”
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A predominant β-CGTase G1 engineered to elucidate the relationship between protein structure and product specificity by Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Raja Abdul Rahman, Raja Noor Zaliha, Md IIlias, Rosli

“…A mutation (Tyr188 → Trp) and a deletion at loop 139–144 showed little effect on product specificity; however, mutagenesis at these sites affected cyclization, coupling and hydrolysis activities as well as the kinetic properties of the mutant CGTase. …”
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