Mechanisms of increased vascular superoxide production in human diabetes mellitus: role of NAD(P)H oxidase and endothelial nitric oxide synthase. by Guzik, T, Mussa, S, Gastaldi, D, Sadowski, J, Ratnatunga, C, Pillai, R, Channon, K

“…METHODS AND RESULTS: Vascular superoxide production was quantified in both saphenous veins and internal mammary arteries from 45 diabetic and 45 matched nondiabetic patients undergoing coronary artery bypass surgery. NAD(P)H-dependent oxidases were important sources of vascular superoxide in both diabetic and nondiabetic patients, but both the activity of this enzyme system and the levels of NAD(P)H oxidase protein subunits (p22phox, p67phox, and p47phox) were significantly increased in diabetic veins and arteries. …”

Systemic and vascular oxidation limits the efficacy of oral tetrahydrobiopterin treatment in patients with coronary artery disease. by Cunnington, C, Van Assche, T, Shirodaria, C, Kylintireas, I, Lindsay, A, Lee, J, Antoniades, C, Margaritis, M, Lee, R, Cerrato, R, Crabtree, M, Francis, J, Sayeed, R, Ratnatunga, C, Pillai, R, Choudhury, R, Neubauer, S, Channon, K

“…METHODS AND RESULTS: Forty-nine patients with coronary artery disease were randomized to receive low-dose (400 mg/d) or high-dose (700 mg/d) BH4 or placebo for 2 to 6 weeks before coronary artery bypass surgery. Vascular function was quantified by magnetic resonance imaging before and after treatment, along with plasma BH4 levels. …”

Relationship of NAD(P)H oxidase activity in human blood vessels to endothelial dysfunction and clinical risk factors by Guzik, T, West, N, Black, E, McDonald, D, Ratnatunga, C, Pillai, R, Channon, K

“…Methods: We used human saphenous vein (HSV) and internal mammary artery (IMA) segments from patients undergoing coronary bypass surgery, to study: (1) Basal and NAD(P)H-stimulated superoxide production, using lucigenin-enhanced chemiluminescence. (2) NO-dependent vasorelaxations, as a bioassay of NO production. (3) Immunochemistry to show endothelial cells and eNOS protein. …”

Systemic and vascular oxidation limits the efficacy of oral tetrahydrobiopterin treatment in patients with coronary artery disease by Cunnington, C, Van Assche, T, Shirodaria, C, Kylintireas, I, Lindsay, A, Lee, J, Antoniades, C, Margaritis, M, Lee, R, Cerrato, R, Crabtree, M, Francis, J, Sayeed, R, Ratnatunga, C, Pillai, R, Choudhury, R, Neubauer, S, Channon, K

“…METHODS AND RESULTS-: Forty-nine patients with coronary artery disease were randomized to receive low-dose (400 mg/d) or high-dose (700 mg/d) BH4 or placebo for 2 to 6 weeks before coronary artery bypass surgery. Vascular function was quantified by magnetic resonance imaging before and after treatment, along with plasma BH4 levels. …”

Association of atrial nicotinamide adenine dinucleotide phosphate oxidase activity with the development of atrial fibrillation after cardiac surgery. by Kim, Y, Kattach, H, Ratnatunga, C, Pillai, R, Channon, K, Casadei, B

“…METHODS: We measured basal and NADPH-stimulated superoxide production in right atrial appendage samples from 170 consecutive patients undergoing conventional coronary artery bypass surgery. Plasma markers of lipid and protein oxidation (thiorbabituric acid-reactive substances, 8-isoprostane, and protein carbonyls) were also measured in blood samples drawn from a central line before surgery and after reperfusion. …”

Association of plasma asymmetrical dimethylarginine (ADMA) with elevated vascular superoxide production and endothelial nitric oxide synthase uncoupling: implications for endotheli... by Antoniades, C, Shirodaria, C, Leeson, P, Antonopoulos, A, Warrick, N, Van-Assche, T, Cunnington, C, Tousoulis, D, Pillai, R, Ratnatunga, C, Stefanadis, C, Channon, K

“…METHODS AND RESULTS: Paired samples of saphenous veins (SVs) and internal mammary arteries (IMAs) were collected from 201 patients undergoing coronary bypass surgery, and serum ADMA was measured pre-operatively. …”