Synaptotagmin-7 in the regulation of hormone secretion. by Teok, Chew Hui.

“…Previous studies showed that the knocked down of Syt-7 in mice resulted in decreased insulin and glucagon secretions and the deletion of Syt-7 in GLUTag cells reduced GLP-1 secretion. …”
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WIP promotes in-vitro invasion ability, anchorage independent growth and EMT progression of A549 lung adenocarcinoma cells by regulating RhoA levels by Salvi, Amrita, Thanabalu, Thirumaran

“…Knocking down WIP expression in A549 cells significantly reduced RhoA levels and WIP was found to interact with RhoA suggesting that WIP might be executing its function by regulating RhoA. …”
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Parkin deficiency exacerbates fasting-induced skeletal muscle wasting in mice by Peker, Nesibe, Sharma, Mridula, Kambadur, Ravi

“…Here, results suggested that knock down of Parkin significantly increases proteolytic activities in skeletal muscle cell line, the C2C12 myotubes. …”
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The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex by Tie, Hieng Chiong, Madugula, Viswanadh, Lu, Lei

“…When endogenous nucleoporins were knocked down and replaced by the exogenously expressed and knockdown-resistant GFP-nucleoporins, the stoichiometry of the nucleoporin was estimated by the ratio of its fluorescence intensity to that of the GFP single molecules. …”
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Cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons. by Zhou, Zimin.

“…We identified four novel genes: Nmnat (Nicotinamide mononucleotide adenylyltransferase); Snap (Soluble NSF attachment protein); Ank2 (Ankyrin 2); Msps (Mini Spindles). Knocking-down these four genes by RNAi result in dendrite pruning defects in ddaC neurons at 16h after puparium formation (16h APF). …”
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The effect of Pf4 filamentous phage infection of its host, pseudomonas aeruginosa. by Ng, Amanda Hui Qi.

“…It is thus interesting to compare the genetic response between wild-type P. aeruginosa and the Pf4 knock-out strain. Here, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to quantify the gene levels of pyoverdine- and multi-drug efflux pump-related genes in P. aeruginosa PAO1 and ∆Pf4. …”
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Regulation of RCC1 and its roles in apoptosis. by Li, Hoi Yeung.

“…As a consequence, nuclear localization signal (NLS) containing proteins, including NF-kB-p65, remain bound to importins α and β in the cytoplasm. Knocking down Mst1 allows resumption of nuclear transport and the nuclear entry of NF-kB-p65, which play important roles in rescuing cells from apoptosis. …”
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Identification of new polyamine targets as stem cell regulators by Ng, Boon Kiat

“…Aqp3 and Zmat4 were overexpressed and Aqp3 was knocked down in mouse ESCs. Zmat4 did not have self-positive feedback regulation. …”
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Role of NMDA receptors in adult neurogenesis and normal development of the dentate gyrus by Åmellem, Ingrid, Yovianto, Glen, Chong, Hai Tarng, Nair, Rajeevkumar Raveendran, Cnops, Vanja, Thanawalla, Ayesha, Tashiro, Ayumu

“…In this study, we investigate this question by examining a region-specific knock-out mouse line that lacks the NR1 gene, which encodes the essential subunit of the NMDA receptors, in granule cells of the dentate gyrus (DG-NR1KO mice). …”
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SRRM2 organizes splicing condensates to regulate alternative splicing by Xu, Shaohai, Lai, Soak-Kuan, Sim, Donald Yuhui, Ang, Warren Shou Leong, Li, Hoi Yeung, Roca, Xavier

“…Using mainly EGFP-SRRM2 knock-in HEK293T cells, we show that SRRM2 forms biomolecular condensates satisfying most hallmarks of liquid-liquid phase separation, including spherical shape, dynamic rearrangement, coalescence and concentration dependence supported by in vitro experiments. …”
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POPX2 is a novel LATS phosphatase that regulates the Hippo pathway by Muhammad Bakhait Rahmat, Zhang, Songjing, Koh, Cheng-Gee

“…As a result, YAP/TAZ are not phosphorylated and are able to translocate into the nucleus to activate target genes involved in cell proliferation. Furthermore, POPX2 knock-out using CRISPR in the highly metastatic MDA-MB-231 breast cancer cells results in decreased cell proliferation and impairment of anchorage independent growth. …”
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Genetic programs of neuronal degeneration in dendrite arborization neurons. by Yap, Kar Wei.

“…We identified four genes, Female sterile (2) Ketel (Fs(2)Ket), Suppressor of variegation 3-9 (Su(var)3-9), Rpt4 and short wing to be involved in dendrite pruning. Knocking down of these genes resulted in the dendrite pruning defect at 16 hour after puparium formation (APF). …”
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Potential role of novel PDZK1 adaptor protein in adipocyte development and lipid metabolism. by Hong, ZhongCheng.

“…SR-BI and key pro-adipogenic regulators such as CCAAT/Enhancer Binding Protein-α, Peroxisome Proliferator-Activated Receptors-γ and Sterol Response Element-Binding-Protein 1c were not downregulated, when PDZK1 was knock-down by short-hairpin RNA. Whether PDZK1 act upstream or downstream of these pro-adipogenic factors requires further test as our control cells did not differentiate well to provide an accurate comparison.…”
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Characterization of zebrafish IKK2 mutants generated by zinc finger nucleases by Ho, Foo Kiong

“…In addition, Tol2 transposon-mediated knock-in was initiated to rescue embryonic lethality observed in homozygous crosses of IKK2 mutants. …”
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Zyxin is involved in fibroblast rigidity sensing and durotaxis by Yip, Ai Kia, Zhang, Songjing, Chong, Lor Huai, Cheruba, Elsie, Woon, Jessie Yong Xing, Chua, Theng Xuan, Goh, Corinna Jie Hui, Yang, Haibo, Tay, Chor Yong, Koh, Cheng-Gee, Chiam, Keng-Hwee

“…Zyxin is a component of the FA that has been implicated in connecting the actin cytoskeleton to the FA. We have found that knocking down zyxin impaired NIH3T3 fibroblast's ability to sense and respond to changes in extracellular matrix in terms of their FA sizes, cell traction stress magnitudes and F-actin organization. …”
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Impaired episodic-like memory in a mouse model of Alzheimer's disease is associated with hyperactivity in prefrontal-hippocampal regions by Tan, Sijie, Tong, Wen Han, Vyas, Ajai

“…Alzheimer's disease (AD) is a degenerative brain disorder with a long prodromal period. An APPNL-G-F knock-in mouse model is a preclinical model to study incipient pathologies during the early stages of AD. …”
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Blocking sense‐strand activity improves potency, safety and specificity of anti‐hepatitis B virus short hairpin RNA by Michler, Thomas, Große, Stefanie, Mockenhaupt, Stefan, Röder, Natalie, Stückler, Ferdinand, Knapp, Bettina, Ko, Chunkyu, Heikenwalder, Mathias, Protzer, Ulrike, Grimm, Dirk

“…Importantly, the novel AAV8 vector co‐expressing anti‐HBV shRNA and TuD outperformed all other strategies regarding efficiency and persistence of HBV knock‐down, thus showing substantial promise for clinical translation.…”
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Viable mice with extensive gene humanization (25-kbp) created using embryonic stem cell/blastocyst and CRISPR/zygote injection approaches by Leidy-Davis, Tiffany, Cheng, Kai, Goodwin, Leslie O., Morgan, Judith L., Juan, Wen Chun, Roca, Xavier, Ong, S. Tiong, Bergstrom, David E.

“…Here, we describe an expansion of the typical DNA size limitations associated with CRISPR knock-in technology, more specifically, the physical extent to which mouse genomic DNA can be replaced with donor (in this case, human) DNA at an orthologous locus by zygotic injection. …”
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Pomc plays an important role in sexual size dimorphism in tilapia by Wan, Zi Yi, Lin, Valerie Chun Ling, Hua, Yue Gen

“…In vitro luciferase assay of the two elements revealed that β-estradiol significantly enhanced the expression of luciferase activity, suggesting that the expression of Pomc is mediated by estrogen. We knocked out Pomc in zebrafish using Crispr/Cas-9. …”
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Dorsal activity of maternal squint is mediated by a non-coding function of the RNA by Lim, Shi Min

“…Due to discrepancies between the genetic nodal mutants and the ablations/antisense knock-down results, the function and mechanism of maternal sqt was debated (Bennett et al., 2007; Gore et al., 2007). …”
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