Recent Advances and Future Perspectives of In Vivo Targeted Delivery of Genome-Editing Reagents to Germ Cells, Embryos, and Fetuses in Mice by Masahiro Sato, Shuji Takabayashi, Eri Akasaka, Shingo Nakamura

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Recent Advances in In Vivo Somatic Cell Gene Modification in Newborn Pups by Shingo Nakamura, Kazunori Morohoshi, Emi Inada, Yoko Sato, Satoshi Watanabe, Issei Saitoh, Masahiro Sato

“…Germline manipulation at the zygote stage using the CRISPR/Cas9 system has been extensively employed for creating genetically modified animals and maintaining established lines. …”
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Recent Advances in the Production of Genome-Edited Rats by Masahiro Sato, Shingo Nakamura, Emi Inada, Shuji Takabayashi

“…In vitro electroporation (EP) of zygotes is next recognized as a simple and rapid method to introduce GE components to produce GE animals. …”
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Recent Genome-Editing Approaches toward Post-Implanted Fetuses in Mice by Shingo Nakamura, Emi Inada, Issei Saitoh, Masahiro Sato

“…There are at least four ways to induce genome editing in individuals: the first is to perform genome editing at the early preimplantation stage, such as fertilized eggs (zygotes), for the creation of whole genetically modified animals; the second is at post-implanted stages, as exemplified by the mid-gestational stages (E9 to E15), for targeting specific cell populations through in utero injection of viral vectors carrying genome-editing components or that of nonviral vectors carrying genome-editing components and subsequent in utero electroporation; the third is at the mid-gestational stages, as exemplified by tail-vein injection of genome-editing components into the pregnant females through which the genome-editing components can be transmitted to fetal cells via a placenta-blood barrier; and the last is at the newborn or adult stage, as exemplified by facial or tail-vein injection of genome-editing components. …”
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