Histamine Receptors: Ex Vivo Functional Studies Enabling the Discovery of Hits and Pathways by Andrea Seldeslachts, Steve Peigneur, Jan Tytgat

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Biochemical and Electrophysiological Characterization of Two Sea Anemone Type 1 Potassium Toxins from a Geographically Distant Population of Bunodosoma caissarum by André J. Zaharenko, Jan Tytgat, José C. Freitas, Adriano M. C. Pimenta, José E. P. W. Bicudo, Gabriela G. Montandon, Juliana S. Cassoli, Bruno Madio, Diego J. B. Orts, Steve Peigneur

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Neurotoxin Merging: A Strategy Deployed by the Venom of the Spider <i>Cupiennius salei</i> to Potentiate Toxicity on Insects by Benjamin Clémençon, Lucia Kuhn-Nentwig, Nicolas Langenegger, Lukas Kopp, Steve Peigneur, Jan Tytgat, Wolfgang Nentwig, Benjamin P. Lüscher

“…Here, we show with the voltage clamp technique in oocytes of <i>Xenopus laevis</i> that a combined application of structural type 1 and type 2 neurotoxins has a much more pronounced cytolytic effect than each of the toxins alone. …”
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Venomous Secretions from Marine Snails of the Terebridae Family Target Acetylcholine Receptors by Cora Wunder, Dietrich Mebs, Jan Tytgat, Steve Peigneur, Annette Nicke, Alexander Kurz, Christian Melaun, Yvonne Kendel, Silke Kauferstein

“…By 2-electrode voltage-clamp technique the gland extracts were tested on Xenopus oocytes expressing nicotinic acetylcholine receptors (nAChRs) of rat neuronal (α3β2, α3β4, α4β2, α4β4, α7) and muscle subtypes (α1β1γδ), and expressing potassium (Kv1.2 and Kv1.3) and sodium channels (Nav1.2, 1.3, 1.4, 1.6). …”
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A Tale of Toxin Promiscuity: The Versatile Pharmacological Effects of Hcr 1b-2 Sea Anemone Peptide on Voltage-Gated Ion Channels by Ernesto Lopes Pinheiro-Junior, Rimma Kalina, Irina Gladkikh, Elena Leychenko, Jan Tytgat, Steve Peigneur

“…As part of its venom components, the Hcr 1b-2 peptide was first described as an ASIC1a and ASIC3 inhibitor. Using <i>Xenopus laevis</i> oocytes and the two-electrode voltage-clamp technique, in the present work we describe the remarkable lack of selectivity of this toxin. …”
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Apamin structure and pharmacology revisited by Alexey I. Kuzmenkov, Steve Peigneur, Joshua A. Nasburg, Konstantin S. Mineev, Konstantin S. Mineev, Maxim V. Nikolaev, Ernesto Lopes Pinheiro-Junior, Alexander S. Arseniev, Alexander S. Arseniev, Heike Wulff, Jan Tytgat, Alexander A. Vassilevski, Alexander A. Vassilevski

“…Here, we investigated the molecular pharmacology of apamin by two-electrode voltage-clamp in Xenopus laevis oocytes and patch-clamp in HEK293, COS7, and CHO cells expressing the studied ion channels, as well as in isolated rat brain neurons. …”
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Purification and Characterization of Bot33: A Non-Toxic Peptide from the Venom of <i>Buthus occitanus tunetanus</i> Scorpion by Rym ElFessi, Oussema Khamessi, Najet Srairi-Abid, Jean-Marc Sabatier, Jan Tytgat, Steve Peigneur, Riadh Kharrat

“…Unexpectedly, this peptide has been subjected to a screening on ion channels expressed in <i>Xenopus laevis</i> oocytes, and it was found that Bot33 has no effect on seven Kv channel subtypes. …”
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WIN55,212-2, a Dual Modulator of Cannabinoid Receptors and G Protein-Coupled Inward Rectifier Potassium Channels by Dongchen An, Steve Peigneur, Jan Tytgat

“…The present study established and validated the functional expression in a <i>Xenopus laevis</i> oocyte expression system of CB1 and CB2 receptors, interacting with heteromeric GIRK1/2 channels and a regulator of G protein signaling, RGS4. …”
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Structure-Function Elucidation of a New α-Conotoxin, MilIA, from <i>Conus milneedwardsi</i> by Steve Peigneur, Prabha Devi, Andrea Seldeslachts, Samuthirapandian Ravichandran, Loïc Quinton, Jan Tytgat

“…</i> The peptide was characterized by electrophysiological screening against several types of cloned nAChRs that were expressed in <i>Xenopus laevis</i> oocytes. MilIA, which is a member of the &#945;3/5 family, is an antagonist of muscle type nAChRs with a high selectivity for muscle versus neuronal subtype nAChRs. …”
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Nicotinic Acetylcholine Receptors Are Novel Targets of APETx-like Toxins from the Sea Anemone <i>Heteractis magnifica</i> by Rimma S. Kalina, Igor E. Kasheverov, Sergey G. Koshelev, Oksana V. Sintsova, Steve Peigneur, Ernesto Lopes Pinheiro-Junior, Roman S. Popov, Victoria E. Chausova, Margarita M. Monastyrnaya, Pavel S. Dmitrenok, Marina P. Isaeva, Jan Tytgat, Sergey A. Kozlov, Emma P. Kozlovskaya, Elena V. Leychenko, Irina N. Gladkikh

“…Hmg 1b-2 potentiated acetylcholine-elicited current in human α7 receptors expressed in <i>Xenopus laevis</i> oocytes. Moreover, the multigene family coding APETx-like peptides library from <i>H. magnifica</i> was described and in silico surface electrostatic potentials of novel peptides were analyzed. …”
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Kunitz-Type Peptides from the Sea Anemone <i>Heteractis crispa</i> Demonstrate Potassium Channel Blocking and Anti-Inflammatory Activities by Irina Gladkikh, Steve Peigneur, Oksana Sintsova, Ernesto Lopes Pinheiro-Junior, Anna Klimovich, Alexander Menshov, Anatoly Kalinovsky, Marina Isaeva, Margarita Monastyrnaya, Emma Kozlovskaya, Jan Tytgat, Elena Leychenko

“…Electrophysiological screening of peptides HCRG1 and HCRG2 from the sea anemone <i>Heteractis crispa</i> on six Kv1.x channel isoforms and insect <i>Shaker</i> IR channel expressed in <i>Xenopus laevis</i> oocytes revealed their potassium channels blocking activity. …”
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