Hypoxia-induced Changes to Integrin α 3 Glycosylation Facilitate Invasion in Epidermoid Carcinoma Cell Line A431 by Ren, Yan, Hao, Piliang, Law, Sai-Kit Alex, Sze, Siu Kwan

“…The results revealed that independent of the protein-level change, N-glycosylation modifications of integrin α 3 (ITGA3) were inhibited by hypoxia, unlike in other integrin subunits. …”
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Structural basis of human full-length kindlin-3 homotrimer in an auto-inhibited state by Bu, Wenting, Levitskaya, Zarina, Loh, Zhi Yang, Jin, Shengyang, Basu, Shibom, Ero, Rya, Yan, Xinfu, Wang, Meitian, Ngan, So Fong Cam, Sze, Siu Kwan, Tan, Suet-Mien, Gao, Yong-Gui

“…Kindlin-1, -2, and -3, directly bind integrin β cytoplasmic tails to regulate integrin activation and signaling. …”
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Hypoxia modulates A431 cellular pathways association to tumor radioresistance and enhanced migration revealed by comprehensive proteomic and functional studies by Ren, Yan, Hao, Piliang, Dutta, Bamaprasad, Cheow, Esther Sok Hwee, Sim, Kae Hwan, Gan, Chee Sian, Lim, Sai Kiang, Sze, Siu Kwan

“…Functional studies confirmed that up-regulation of glycolysis, integrin, glycoprotein synthesis, and down-regulation of STAT1 pathways during hypoxia enhanced metastastic activity of A431 cells. …”
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In-vivo structure-based studies on epidermal growth factor receptor (EGFR) signalling in cancer. by Sze, Siu Kwan.

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The methyltransferase Ezh2 controls cell adhesion and migration through direct methylation of the extranuclear regulatory protein talin by Ginhoux, Florent, Su, I-hsin, Gunawan, Merry, Venkatesan, Nandini, Loh, Jia Tong, Wong, Jong Fu, Berger, Heidi, Neo, Wen Hao, Li, Liang Yao Jackson, La Win, Myint Khun, Yau, Yin Hoe, Guo, Tiannan, See, Peter Chi Ee, Yamazaki, Sayuri, Chin, Keh Chuang, Gingras, Alexandre R., Shochat, Susana Geifman, Ng, Lai Guan, Sze, Siu Kwan

“…Here we found that Ezh2 regulated the integrin signaling and adhesion dynamics of neutrophils and dendritic cells (DCs). …”
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Monocyte adhesion to atherosclerotic matrix proteins is enhanced by Asn-Gly-Arg deamidation by Dutta, Bamaprasad, Park, Jung Eun, Kumar, Subodh, Hao, Piliang, Gallart-Palau, Xavier, Serra, Aida, Ren, Yan, Sorokin, Vitaly, Lee, Chuen Neng, Ho, Hee Hwa, de Kleijn, Dominique, Sze, Siu Kwan

“…Together, these findings confirm a critical role for isoDGR motifs in mediating leukocyte adhesion to the ECM via integrin αvβ3 and suggest that protein deamidation may promote the pathological progression of human atherosclerosis by enhancing monocyte recruitment to developing plaques.…”
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Aging-induced isoDGR-modified fibronectin activates monocytic and endothelial cells to promote atherosclerosis by Park, Jung Eun, JebaMercy, Gnanasekaran, Pazhanchamy, Kalailingam, Guo, Xue, Ngan, SoFong Cam, Liou, Ken Cheng Kang, Soe, Lynn EinSi, Ng, Ser Sue, Meng, Wei, Lim, Su Chi, Leow, Melvin Khee-Shing, Richards, A. Mark, Pennington, Daniel J., de Kleijn, Dominique P. V., Sorokin, Vitaly, Ho, Hee Hwa, McCarthy, Neil E., Sze, Siu Kwan

“…IsoDGR-modified fibronectin also induced endothelial cell expression of integrin β1 to further enhance cellular adhesion and matrix deposition. …”
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Discovery of prognostic biomarker candidates of lacunar infarction by quantitative proteomics of microvesicles enriched plasma by Datta, Arnab, Chen, Christopher P., Sze, Siu Kwan

“…Bioinformatics analysis and data mining revealed up-regulation of brain-specific proteins including myelin basic protein, proteins of coagulation cascade (e.g., fibrinogen alpha chain, fibrinogen beta chain) and focal adhesion (e.g., integrin alpha-IIb, talin-1, and filamin-A) while albumin was down-regulated in both groups of patients with adverse outcome. …”
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Paxillin interactome identified by SILAC and label-free approaches coupled to TurboID sheds light on the compositions of focal adhesions in mouse embryonic stem cells by He, Qianqian, Sze, Siu Kwan, Ng, Kai Soon, Koh, Cheng-Gee

“…Self-renewal and differentiation of mouse embryonic stem cells (mESCs) are greatly affected by the extracellular matrix (ECM) environment; the composition and stiffness of which are sensed by the cells via integrin-associated focal adhesions (FAs) which link the cells to the ECM. …”
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Immunotherapy targeting isoDGR-protein damage extends lifespan in a mouse model of protein deamidation by Kalailingam, Pazhanichamy, Mohd-Kahliab, Khalilatul-Hanisah, Ngan, SoFong Cam, Iyappan, Ranjith, Melekh, Evelin, Lu, Tian, Zien, Gan Wei, Sharma, Bhargy, Guo, Tiannan, MacNeil, Adam J., MacPherson, Rebecca Ek, Tsiani, Evangelia Litsa, O'Leary, Deborah D., Lim, Kah-Leong, Su, I Hsin, Gao, Yong-Gui, Richards, A. Mark, Kalaria, Raj N., Chen, Christopher P., McCarthy, Neil E., Sze, Siu Kwan

“…We previously reported that age-linked damage to amino acid sequence NGR (Asn-Gly-Arg) results in "gain-of-function" conformational switching to isoDGR (isoAsp-Gly-Arg). This integrin-binding motif activates leukocytes and promotes chronic inflammation, which are characteristic features of age-linked cardiovascular disorders. …”
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GSK3β interacts with CRMP2 and Notch1 and controls T-cell motility by Mobashar Hussain Urf Turabe Fazil, Prasannan, Praseetha, Wong, Brandon Han Siang, Kottaiswamy, Amuthavalli, Nur Syazwani Mohamed Salim, Sze, Siu Kwan, Verma, Navin Kumar

“…Inhibition of GSK3β by CHIR-99021 or siRNA-mediated knockdown augmented the migratory behavior of human T-lymphocytes stimulated via an engagement of the T-cell integrin LFA-1 with its ligand ICAM-1. Proteomics and protein network analysis revealed ongoing interactions among GSK3β, the surface receptor Notch1 and the cytoskeletal regulator CRMP2. …”
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The steroidal lactone withaferin A impedes T-cell motility by inhibiting the kinase ZAP70 and subsequent kinome signaling by Mobashar Hussain Urf Turabe Fazil, Chirumamilla, Chandra Sekhar, Perez-Novo, Claudina, Wong, Brandon Han Siang, Kumar, Sunil, Sze, Siu Kwan, Berghe, Wim Vanden, Verma, Navin Kumar

“…We found that WFA dose-dependently (within the concentration range of 0.3-1.25 μM) inhibited the ability of human T-cells to migrate via cross-linking of the lymphocyte function-associated antigen-1 (LFA-1) integrin with its ligand, intercellular adhesion molecule 1 (ICAM-1). …”
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