A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry. by Yan, J, Wait, R, Berkelman, T, Harry, R, Westbrook, J, Wheeler, C, Dunn, M

“…We show that tryptic digests of proteins visualized by the modified stain afford excellent mass spectra by both matrix-assisted laser desorption/ionization and tandem electrospray ionization. …”

A proteomic investigation of isolated soy proteins with variable effects in experimental and clinical studies. by Gianazza, E, Eberini, I, Arnoldi, A, Wait, R, Sirtori, C

“…After two-dimensional electrophoresis, identities of the protein components (globulin subunits and their breakdown products) were established by matrix-assisted laser desorption/ionization mass spectrometry. The soy concentrates (Cholsoy/Croksoy), which were used in most of the Italian and Swiss studies in which reductions in cholesterolemia occurred, exhibited a predominance of breakdown products of the 7S globulin and mainly intact 11S globulin subunits. …”

Separation and identification of rat skeletal muscle proteins using two-dimensional gel electrophoresis and mass spectrometry. by Yan, J, Harry, R, Wait, R, Welson, S, Emery, P, Preedy, V, Dunn, M

“…We used immobilized pH gradients of 3-10 (non-linear) and 4-7 (linear), and matrix assisted laser desorption/ionization--time of flight mass spectrometry for protein identification by peptide mass fingerprinting. …”

Relationships between the N-glycan structures and biological activities of recombinant human erythropoietins produced using different culture conditions and purification procedures... by Yuen, C, Storring, P, Tiplady, R, Izquierdo, M, Wait, R, Gee, C, Gerson, P, Lloyd, P, Cremata, J

“…The N-glycan structures of these EPOs were analysed using a recently developed profiling procedure and identified using matrix-assisted laser desorption ionization mass spectrometry. The specific activities of each of the EPOs were estimated by in vivo and in vitro mouse bioassays. …”

A reference map of human lung MRC-5 fibroblast proteins using immobilized pH gradient-isoelectric focusing-based two-dimensional electrophoresis. by Leung, K, Wait, R, Welson, S, Yan, J, Abraham, D, Black, C, Pearson, J, Dunn, M

“…In this map a total of 98 protein spots were identified by comparative searches of the nucleotide and protein database using peptide masses obtained by matrix-assisted laser desorption/ionization time of flight following trypsin digestion. …”

Structures of the O-linked oligosaccharides of a complex glycoconjugate from Pseudallescheria boydii. by Pinto, MR, Gorin, P, Wait, R, Mulloy, B, Barreto-Bergter, E

“…The major oligosaccharide from fraction 1 was characterized by a combination of techniques including electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI MS/MS), matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), nuclear magnetic resonance (NMR), and methylation gas-liquid chromatography-mass spectrometry (GC-MS) analysis. …”

A combined radiolabelling and silver staining technique for improved visualisation, localisation, and identification of proteins separated by two-dimensional gel electrophoresis. by Westbrook, J, Yan, J, Wait, R, Dunn, M

“…Protein identification of superimposed spots is described by peptide mass fingerprinting and database searching using matrix-assisted laser desorption/ionization-time of flight mass spectrometry and by peptide sequencing using tandem MS by hybrid quadrupole/orthogonal acceleration time of flight MS (Q-TOF).…”

Fluorescence two-dimensional difference gel electrophoresis and mass spectrometry based proteomic analysis of Escherichia coli. by Yan, J, Devenish, A, Wait, R, Stone, T, Lewis, S, Fowler, S

“…Using matrix-assisted laser desorption/ionization-time of flight and quadrupole-time of flight mass spectrometry a total of 179 differentially expressed protein spots were identified. …”

F-actin capping (CapZ) and other contractile saphenous vein smooth muscle proteins are altered by hemodynamic stress: a proteonomic approach. by McGregor, E, Kempster, L, Wait, R, Gosling, M, Dunn, M, Powell, J

“…Previously, we characterized and mapped 129 proteins in human saphenous vein medial smooth muscle using two-dimensional (2-D) PAGE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. …”

Proteomic analysis of the endoplasmic reticulum from developing and germinating seed of castor (Ricinus communis). by Maltman, D, Simon, W, Wheeler, C, Dunn, M, Wait, R, Slabas, A

“…Several major components have been identified from 1-D gels by N-terminal sequencing and matrix-assisted laser desorption/ionization (MALDI) peptide mass fingerprints. …”

High pressure effects step-wise altered protein expression in Lactobacillus sanfranciscensis. by Drews, O, Weiss, W, Reil, G, Parlar, H, Wait, R, Görg, A

“…Two of them (approx. values: pI 4.0 and 4.2, respectively; M(r) approximately 15 000) have almost identical matrix-assisted laser desorption/ionization-time of flight mass spectrometry spectra and were identified by liquid chromatography-tandem mass spectrometry as putative homologs/paralogs to cold shock proteins of Lactococcus lactis. …”