Distinct origins of adult and embryonic blood in Xenopus. by Ciau-Uitz, A, Walmsley, M, Patient, R

“…Whether embryonic and adult blood derive from a single (yolk sac) or dual (yolk sac plus intraembryonic) origin is controversial. Here, we show, in Xenopus, that the yolk sac (VBI) and intraembryonic (DLP) blood compartments derive from distinct blastomeres in the 32-cell embryo. …”

A role for GATA5 in Xenopus endoderm specification. by Weber, H, Symes, C, Walmsley, M, Rodaway, A, Patient, R

“…Here we show that GATA5, a zinc-finger transcription factor, is expressed in the yolk-rich vegetal cells of Xenopus embryos from the early gastrula stage onwards, when these cells become committed to form endoderm. …”

The origins and programming of adult and embryonic blood in xenopus and zebrafish. by Patient, R, Walmsley, M, Ciau-Uitz, A, Gering, M, Pinheiro, P, Patterson, L, Nastos, T, Gottgens, B, Green, T, Yamada, Y, Rabbitts, T

Tracking and programming early hematopoietic cells in Xenopus embryos. by Walmsley, M, Ciau-Uitz, A, Patient, R

“…The fates of lineage labeled hematopoietic precursor populations in Xenopus embryos are followed by use of in situ hybridization, looking for overlap between lineage labeled cells and in situ probes specific for known cell populations or states of differentiation. …”

Adult and embryonic blood and endothelium derive from distinct precursor populations which are differentially programmed by BMP in Xenopus. by Walmsley, M, Ciau-Uitz, A, Patient, R

“…These results indicate that both adult and the anterior component of embryonic blood in Xenopus embryos derive from populations of progenitors that also give rise to endothelial cells. …”

Fibroblast growth factor controls the timing of Scl, Lmo2, and Runx1 expression during embryonic blood development. by Walmsley, M, Cleaver, D, Patient, R

“…The anterior (a) and posterior (p) ventral blood islands (VBIs) in Xenopus are derived from opposite sides of the pregastrula embryo. …”

Stochastic specification of primordial germ cells from mesoderm precursors in axolotl embryos. by Chatfield, J, O'Reilly, M, Bachvarova, R, Ferjentsik, Z, Redwood, C, Walmsley, M, Patient, R, Loose, M, Johnson, A

“…A common feature of development in most vertebrate models is the early segregation of the germ line from the soma. For example, in Xenopus and zebrafish embryos primordial germ cells (PGCs) are specified by germ plasm that is inherited from the egg; in mice, Blimp1 expression in the epiblast mediates the commitment of cells to the germ line. …”

Fli1 acts at the top of the transcriptional network driving blood and endothelial development. by Liu, F, Walmsley, M, Rodaway, A, Patient, R

“…However, loss-of-function phenotypes in mice, although suffering both blood and endothelial defects, have thus far precluded the conclusion that Fli1 is essential for these two lineages [11, 12]. By using Xenopus and zebrafish embryos, we show that loss of Fli1 function results in a substantial reduction or absence of hemangioblasts, revealing an absolute requirement. …”