Apolipoprotein E is required for brain iron homeostasis in mice by Juan Ma, Qian Guo, Meng-Qi Shen, Wei Li, Qi-Xin Zhong, Zhong-Ming Qian

Subjects: “…Apolipoprotein E knock-out (ApoE KO/ApoE−/−) mice…”
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WNTA5-mediated miR-374a-5p regulates vascular smooth muscle cell phenotype transformation and M1 macrophage polarization impacting intracranial aneurysm progression by Zengshi Li, Junqiang Huang, Lijian Yang, Xi Li, Wei Li

“…Subsequently, miR-374a-5p was knocked down or overexpressed. We investigated the effects of miR-374a-5p on phenotypic conversion, and in vivo experiments were also carried out to verify the findings. …”
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New Insights into Abnormal Combustion Phenomena Induced by Diesel Spray-Wall Impingement under Engine-Relevant Conditions by Zhijie Li, Jie Pan, Wei Li, Xiangting Wang, Haiqiao Wei, Jiaying Pan

“…Under such operating conditions, heavy-duty diesel engines often suffer from serious abnormal combustion, such as knocking combustion, which results in piston crown breakdown and cylinder head erosion. …”
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S-Allylmercaptocysteine improves alcoholic liver disease partly through a direct modulation of insulin receptor signaling by Pingping Luo, Ming Zheng, Rui Zhang, Hong Zhang, Yingxia Liu, Wei Li, Xiaoming Sun, Qian Yu, George L. Tipoe, Jia Xiao

“…By using biophysical assays and molecular docking, we demonstrated that SAMC directly targeted insulin receptor (INSR) protein on the cell membrane and then restored downstream IRS-1/AKT/GSK3β signaling. Liver-specific knock-down in mice and siRNA-mediated knock-down in AML-12 cells of Insr significantly impaired SAMC (250 μmol/L in cells)-mediated protection. …”
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Novel Vitamin D3 Hydroxymetabolites Require Involvement of the Vitamin D Receptor or Retinoic Acid-Related Orphan Receptors for Their Antifibrogenic Activities in Human Fibroblasts by Zorica Janjetovic, Shariq Qayyum, Sivani B. Reddy, Ewa Podgorska, S. Gates Scott, Justyna Szpotan, Alisa A. Mobley, Wei Li, Vijay K. Boda, Senthilkumar Ravichandran, Robert C. Tuckey, Anton M. Jetten, Andrzej T. Slominski

“…This effect was reversed in cells where VDR or RORs were knocked down where the inhibition of collagen production and the expression of anti-fibrotic genes in response to the hydroxymetabolites was abrogated, along with ablation of their anti-inflammatory action. …”
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TMEM43 Protects against Sepsis-Induced Cardiac Injury via Inhibiting Ferroptosis in Mice by Zhen Chen, Zhe Cao, Feng Gui, Mengli Zhang, Xian Wu, Huan Peng, Bo Yu, Wei Li, Fen Ai, Jun Zhang

“…Mice were also subjected to AAV9-shTMEM43 to knock down TMEM43 or AAV9-TMEM43 to overexpress TMEM43 in hearts. …”
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Lupus susceptibility gene Esrrg modulates regulatory T cells through mitochondrial metabolism by Wei Li, Minghao Gong, Yuk Pheel Park, Ahmed S. Elshikha, Seung-Chul Choi, Josephine Brown, Nathalie Kanda, Wen-I Yeh, Leeana Peters, Anton A. Titov, Xiangyu Teng, Todd M. Brusko, Laurence Morel

“…We found that the expression of human ESRRG, which is high in Tregs, was lower in CD4+ T cells from patients with lupus than in healthy controls. Finally, knocking down ESRRG in Jurkat T cells decreased their metabolism. …”
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The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair by Tao Huang, Shenli Yuan, Lei Gao, Mengjing Li, Xiaochen Yu, Jianhong Zhan, Yingying Yin, Chao Liu, Chuanxin Zhang, Gang Lu, Wei Li, Jiang Liu, Zi-Jiang Chen, Hongbin Liu

“…We generated H3K4me3 reader-dead Zcwpw1 mutant mice and found that their spermatocytes were arrested at the pachytene-like stage, which phenocopies the Zcwpw1 knock–out mice. Based on various ChIP-seq and immunofluorescence analyses using several mutants, we found that Zcwpw1's occupancy on chromatin is strongly promoted by the histone-modification activity of PRDM9. …”
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ACO2 deficiency increases vulnerability to Parkinson’s disease via dysregulating mitochondrial function and histone acetylation-mediated transcription of autophagy genes by Junge Zhu, Fanxi Xu, Hong Lai, Huiyao Yuan, Xu-Ying Li, Junya Hu, Wei Li, Lei Liu, Chaodong Wang

“…Here we revealed that the peripheral ACO2 activity was significantly decreased in PD patients and associated with their onset age and disease durations. The knock-in mouse and Drosophila models with the A252T variant displayed aggravated motor deficits and DA neuron degeneration after 6-OHDA and rotenone-induction, and the ACO2 knockdown or blockade cells showed features of mitochondrial and autophagic dysfunction. …”
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miR-10a inhibits cell proliferation and promotes cell apoptosis by targeting BCL6 in diffuse large B-cell lymphoma by Qian Fan, Xiangrui Meng, Hongwei Liang, Huilai Zhang, Xianming Liu, Lanfang Li, Wei Li, Wu Sun, Haiyang Zhang, Ke Zen, Chen-Yu Zhang, Zhen Zhou, Xi Chen, Yi Ba

“…By overexpressing or knocking down miR-10a in DLBCL cells, we experimentally validated that miR-10a directly recognizes the 3′-UTR of the BCL6 transcript and regulated BCL6 expression. …”
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High-efficiency targeted transgene integration via primed micro-homologues by Chenxin Wang, Sen Fang, Yangcan Chen, Na Tang, Guanyi Jiao, Yanping Hu, Jing Li, Qingtong Shan, Xin Wang, Guihai Feng, Qi Zhou, Wei Li

“…Here, we exploit prime editors to devise a robust knock-in (KI) strategy named primed micro-homologues-assisted integration (PAINT), which utilizes reverse-transcribed single-stranded micro-homologues to boost targeted KIs in different types of cells. …”
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<i>Ss</i>NEP2 Contributes to the Virulence of <i>Sclerotinia sclerotiorum</i> by Chenghuizi Yang, Wei Li, Xingchuan Huang, Xianyu Tang, Lei Qin, Yanan Liu, Yunong Xia, Zhihong Peng, Shitou Xia

“…Here, we generated <i>S. sclerotiorum</i> necrosis and ethylene-inducible peptide 2 (<i>SsNEP2</i>) deletion mutant through homologous recombination and found that <i>Ss</i>NEP2 contributes to the virulence of <i>S. sclerotiorum</i> without affecting the development of mycelia, the formation of appressoria, or the secretion of oxalic acid. Although knocking out <i>SsNEP2</i> did not affect fungal sensitivity to oxidative stress, it did lead to decreased accumulation of reactive oxygen species (ROS) in <i>S. sclerotiorum</i>. …”
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Melatonin alleviates alcoholic liver disease via EGFR–BRG1–TERT axis regulation by Zhaodi Che, Yali Song, Chengfang Xu, Wei Li, Zhiyong Dong, Cunchuan Wang, Yixing Ren, Kwok-Fai So, George L. Tipoe, Fei Wang, Jia Xiao

“…Biophysical assay found that epidermal growth factor receptor (EGFR) on the hepatocyte surface was a direct binding and regulating target of MLT. Liver specific knock-down of Tert or Egfr in the ALD mice model impaired MLT-mediated liver protection, partly through the regulation of nuclear brahma-related gene-1 (BRG1). …”
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High concentrations of soluble endoglin can inhibit BMP9 signaling in non-endothelial cells by Clara Andersson-Rusch, Bin Liu, Ingrid Quist-Løkken, Paul D. Upton, Oddrun Elise Olsen, Hanne Hella, Xudong Yang, Zhen Tong, Nicholas W. Morrell, Toril Holien, Wei Li

“…We have shown that while loss of cell surface ENG decreases BMP9 signaling in endothelial cells, knocking down ENG in blood cancer cells enhances BMP9 signaling. …”
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Speckle-type POZ protein could play a potential inhibitory role in human renal cell carcinoma by Zhi Chen, Zuan Li, Chunlin Li, Bingcai Li, Haojian Wang, Deyong Nong, Ximing Li, Guihai Huang, Junhao Lin, Wei Li

“…Methods Here, SPOP was overexpressed by lentiviral vector transfection in ACHN and Caki-1 cells, and SPOP was knocked down in Caki-2 cells with similar transfection methods. …”
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