DNMT3A low-expression is correlated to poor prognosis in childhood B-ALL and confers resistance to daunorubicin on leukemic cells by Weijing Li, Shuguang Liu, Chanjuan Wang, Lei Cui, Xiaoxi Zhao, Wei Liu, Ruidong Zhang, Zhigang Li

“…Correlations with relapse-free survival (RFS) and common clinical characteristics were analyzed. DNMT3A was stably knocked out by CRISPR/Cas9 gene editing technology in Reh and 697 B-ALL cell lines. …”
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Combined analyses of RNA-sequence and Hi-C along with GWAS loci-A novel approach to dissect keloid disorder genetic mechanism. by Jia Huang, Xiaobo Zhou, Wenbo Wang, Guangdong Zhou, WenJie Zhang, Zhen Gao, Xiaoli Wu, Wei Liu

“…Among them, the functions of six molecules for cell proliferation, cell cycle and apoptosis were particularly examined and confirmed by overexpressing and knocking-down assays. This study firstly revealed unknown key biomarkers and pathways in keloid lesions using RNA-sequence and previously reported mutation loci, indicating a feasible approach to reveal the genetic contribution to keloid disorder and possibly to other diseases that are failed by GWAS analysis alone.…”
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Start codon disruption with CRISPR/Cas9 prevents murine Fuchs’ endothelial corneal dystrophy by Hironori Uehara, Xiaohui Zhang, Felipe Pereira, Siddharth Narendran, Susie Choi, Sai Bhuvanagiri, Jinlu Liu, Sangeetha Ravi Kumar, Austin Bohner, Lara Carroll, Bonnie Archer, Yue Zhang, Wei Liu, Guangping Gao, Jayakrishna Ambati, Albert S Jun, Balamurali K Ambati

“…A single intraocular injection of an adenovirus encoding both the Cas9 gene and guide RNA (Ad-Cas9-Col8a2gRNA) efficiently knocked down mutant COL8A2 expression in corneal endothelial cells, prevented endothelial cell loss, and rescued corneal endothelium pumping function in adult Col8a2 mutant mice. …”
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LncRNA LINK‐A Remodels Tissue Inflammatory Microenvironments to Promote Obesity by Yu Chen, Hui Chen, Ying Wang, Fangzhou Liu, Xiao Fan, Chengyu Shi, Xinwan Su, Manman Tan, Yebin Yang, Bangxing Lin, Kai Lei, Lei Qu, Jiecheng Yang, Zhipeng Zhu, Zengzhuang Yuan, Shanshan Xie, Qinming Sun, Dante Neculai, Wei Liu, Qingfeng Yan, Xiang Wang, Jianzhong Shao, Jian Liu, Aifu Lin

“…This study has used genetic screening to identify an obesity‐associated human lncRNA LINK‐A as a critical molecule bridging the metabolic microenvironment and energy expenditure in vivo by establishing the HFD‐induced obesity knock‐in (KI) mouse model. Mechanistically, HFD LINK‐A KI mice induce the infiltration of inflammatory factors, including IL‐1β and CXCL16, through the LINK‐A/HB‐EGF/HIF1α feedback loop axis in a self‐amplified manner, thereby promoting the adipose tissue microenvironment remodeling and adaptive thermogenesis disorder, ultimately leading to obesity and insulin resistance. …”
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Identification of HMGB2 associated with proliferation, invasion and prognosis in lung adenocarcinoma via weighted gene co-expression network analysis by Xie Qiu, Wei Liu, Yifan Zheng, Kai Zeng, Hao Wang, Haijun Sun, Jianhua Dai

“…Besides, over-expression of HMGB2 promoted A549 cells proliferation and migration while knocking down of HMGB2 suppressed the tumor promoting effect. …”
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A novel recombination protein C12ORF40/REDIC1 is required for meiotic crossover formation by Suixing Fan, Yuewen Wang, Hanwei Jiang, Xiaohua Jiang, Jianteng Zhou, Yuying Jiao, Jingwei Ye, Zishuo Xu, Yue Wang, Xuefeng Xie, Huan Zhang, Yang Li, Wei Liu, Xiangjun Zhang, Hui Ma, Baolu Shi, Yuanwei Zhang, Muhammad Zubair, Wasim Shah, Zhipeng Xu, Bo Xu, Qinghua Shi

“…Spread mouse spermatocyte fluorescence immunostaining showed that REDIC1 forms discrete foci between the paired regions of homologous chromosomes depending on strand invasion and colocalizes with MSH4 and later with MLH1 at the crossover sites. Redic1 knock-in (KI) mice homozygous for mutation c.232_233insTT are infertile in both sexes due to insufficient crossovers and consequent meiotic arrest, which is also observed in our patients. …”
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Combined analyses of RNA-sequence and Hi-C along with GWAS loci—A novel approach to dissect keloid disorder genetic mechanism by Jia Huang, Xiaobo Zhou, Wenbo Wang, Guangdong Zhou, WenJie Zhang, Zhen Gao, Xiaoli Wu, Wei Liu

“…Among them, the functions of six molecules for cell proliferation, cell cycle and apoptosis were particularly examined and confirmed by overexpressing and knocking-down assays. This study firstly revealed unknown key biomarkers and pathways in keloid lesions using RNA-sequence and previously reported mutation loci, indicating a feasible approach to reveal the genetic contribution to keloid disorder and possibly to other diseases that are failed by GWAS analysis alone. …”
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FBXO5-mediated RNF183 degradation prevents endoplasmic reticulum stress-induced apoptosis and promotes colon cancer progression by Jing Ji, Aixin Jing, Yuanyuan Ding, Xinhui Ma, Qilan Qian, Ting Geng, Wenhao Cheng, Meiqi Zhang, Qian Sun, Shaojie Ma, Xiujun Wang, Qing Yuan, Menghan Xu, Jingting Qin, Lin Ma, Jiayan Yang, Jingliang He, Qianming Du, Mengbei Xia, Yuting Xu, Ziyun Chen, Lan Zhu, Wei Liu, Shunfang Liu, Bin Liu

“…Reversal of the apoptosis defects caused by FBXO5 deficiency in colorectal cancer cells can be achieved by knocking down RNF183 in FBXO5-deficient cells. Functionally, we observed significant upregulation of FBXO5 in colon cancer tissues, and its silencing suppresses tumor occurrence in vivo. …”
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Construction of an alternative NADPH regeneration pathway improves ethanol production in Saccharomyces cerevisiae with xylose metabolic pathway by Yali Qiu, Wei Liu, Meiling Wu, Haodong Bao, Xinhua Sun, Qin Dou, Hongying Jia, Weifeng Liu, Yu Shen

“…However, glucose and xylose share flux in the pentose phosphate pathway (PPP) and glycolysis pathway (EMP), with glucose having a competitive advantage in the shared metabolic pathways. In this work, we knocked down ZWF1 to preclude glucose from entering the PPP. …”
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BM-MSCs overexpressing the Numb enhance the therapeutic effect on cholestatic liver fibrosis by inhibiting the ductular reaction by Yan-nan Xu, Wen Xu, Xu Zhang, Dan-yang Wang, Xin-rui Zheng, Wei Liu, Jia-mei Chen, Gao-feng Chen, Cheng-hai Liu, Ping Liu, Yong-ping Mu

“…In vitro, the Numb gene was overexpressed or knocked down in the WB-F344 cell line by lentivirus transfection, Then, cells were subjected immunofluorescence staining and the detection of mRNA levels of related factors, which provided further evidence supporting the results from in vivo experiments. …”
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The adcA and lmb Genes Play an Important Role in Drug Resistance and Full Virulence of Streptococcus suis by Mingzheng Peng, Yuanyuan Xu, Beibei Dou, Fengming Yang, Qiyun He, Zewen Liu, Ting Gao, Wei Liu, Keli Yang, Rui Guo, Chang Li, Yongxiang Tian, Danna Zhou, Weicheng Bei, Fangyan Yuan

“…Here, we investigated the contribution of zinc to the drug resistance and pathogenesis of S. suis. We knocked out the genes of AdcACB and Lmb, two Zn-binding lipoproteins. …”
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Integrated ATAC-Seq and RNA-Seq Data Analysis to Reveal <i>OsbZIP14</i> Function in Rice in Response to Heat Stress by Fuxiang Qiu, Yingjie Zheng, Yao Lin, Samuel Tareke Woldegiorgis, Shichang Xu, Changqing Feng, Guanpeng Huang, Huiling Shen, Yinying Xu, Manegdebwaoga Arthur Fabrice Kabore, Yufang Ai, Wei Liu, Huaqin He

“…Through comprehensive bioinformatics analysis, we demonstrated that <i>OsbZIP14</i>, one of the key heat-responsive TF genes, contained a basic-leucine zipper domain and primarily functioned as a nuclear TF with transcriptional activation capability. By knocking out the <i>OsbZIP14</i> gene in the rice cultivar Zhonghua 11, we observed that the knockout mutant <i>OsbZIP14</i> exhibited dwarfism with reduced tiller during the grain-filling stage. …”
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