Validation of internal reference genes for relative quantitation studies of gene expression in human laryngeal cancer by Xiaofeng Wang, Jinting He, Wei Wang, Ming Ren, Sujie Gao, Guanjie Zhao, Jincheng Wang, Qiwei Yang

Subjects: “…Reverse transcription quantitative polymerase chain reaction…”
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Log (Lactobacillus crispatus/ Gardnerella vaginalis): a new indicator of diagnosing bacterial vaginosis by Tongyang Deng, Anquan Shang, Ying Zheng, Lianzhen Zhang, Hong Sun, Wei Wang

“…Abbreviation: log L. crispatus/G. vaginalis (logL/G); Bacterial vaginosis (BV); vaginal secretions (VSs); polymerase chain reaction (PCR); rRNA-sequencing (rRNA-seq); real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR); operational taxonomic unit (OTU); non-metric multidimensional scaling (NMDS); receiver operating characteristic (ROC).…”
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Transcriptome responses of RNAi-mediated ETH knockdown in Scylla paramamosain at different premolt substages by Siuming-Francis Chan, Qi-Qiao Wen, Chun-Mei Ao, Wei Wang, Wei Wang, Cheng-Gui Wang, Yan-Fei Zhao

“…Real-time quantitative polymerase chain reaction (RT-qPCR) and semiquantitative polymerase chain reaction (RT-PCR) results showed a significant knockdown of ETH at D0 stage, whereas a significant increase was shown conversely in crabs at D2 substage after the injection of dsETH. …”
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LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis by Yanqing Feng, Gang Wei, Linfei Zhang, Huadong Zhou, Wei Wang, Peng Guo, Caitao Cheng, Lei Ji, Qinghe Cai, Yong Feng, Huahua Tu

“…The profiles of DARS-AS1 and Cytoskeleton associated protein 2 (CKAP2) in 50 HCC tissues and non-tumor tissues were examined by real-time quantitative polymerase chain reaction (RT-qPCR). DARS-AS1 and CKAP2 overexpression and/or knockdown cell models were established. …”
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Prognostic factors and Doxorubicin involved in malignant progression of meningioma by Xulei Huo, Lairong Song, Ke Wang, Hongyi Wang, Da Li, Huan Li, Wei Wang, Yali Wang, Lei Chen, Zongmao Zhao, Liang Wang, Zhen Wu

“…The external dataset GEO136661 and quantitative Real-time Polymerase Chain Reaction were used to verify the prognostic factors. has-miR-3605-5p, hsa-miR-664b-5p, PNRC2, BTBD8, EXTL2, SLFN13, DGKD, NSD2, and BVES were closed with malignant progression. …”
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Increased IL-1α expression is correlated with bladder cancer malignant progression by Shi-Jie Yao, Hong-Shun Ma, Guang-Ming Liu, Yue Gao, Wei Wang

“…The gene and protein expression was tested by reverse transcription quantitative polymerase chain reaction (RT-q-PCR) and western blot, respectively. …”
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MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy by Pengwei Liu, Lin Li, Wei Wang, Chiyi He, Chunfang Xu

“…The MST4 expression level in GC cells was measured by western blotting and quantitative real-time polymerase chain reaction. Moreover, the regulatory mechanism of MST4 was investigated in vitro and in vivo. …”
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Correlation of DNA methylation of DNMT3A and TET2 with oral squamous cell carcinoma by Xueming Li, Zaikun Li, Qingxi Gao, Yanan Peng, Yang Yu, Tenglong Hu, Wei Wang

“…DNA methylation and protein expression levels of DNMT3A and TET2 in tissues were analyzed with methylation-specific polymerase chain reaction (MSP) and western blotting. To evaluate the effects of DNMT3A and TET2 on the biological characteristics of OSCC, cell proliferation was assessed with 5-ethynyl-2'-deoxyuridine, and cell migration capacity was quantified with wound healing and transwell assays. …”
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4-Octyl itaconate treatment inhibits mitochondrial dysfunction and ER stress via stimulating SIRT1 expression in vitro and attenuates osteoarthritis process in murine DMM model in... by Ziping Yu, Zhao Zhang, Xuancheng Zhang, Junduo Bao, Hualin Li, Jiapei Yu, Ning Shi, Fulong Nan, Liang Cao, Chenghui Li, Wei Wang

“…Using western blot, real-time quantitative reverse transcription polymerase chain reaction and immunofluorescence staining, we found that 125 μM 4-OI can inhibit apoptosis, ECM degeneration, mitochondrial dysfunction, ER stress and abundant accumulation of ROS in chondrocytes. …”
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Metabolic Syndrome Is Associated with Poor Omicron Infection Prognosis While Inactivated Vaccine Improves the Outcome of Coronavirus Disease 2019 among Chinese Inhabitants: A Retro... by Ying Liu, Dong Chen, Junfeng Li, Wei Wang, Rongfeng Han, Shanshan Cui, Suqing Bao

“…The results showed that MetS was strongly associated with Intensive Unit Care (ICU) admission, Polymerase Chain Reaction (PCR) re-positivity, and severe COVID-19. …”
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Hsa_circ_0001615 downregulation inhibits esophageal cancer development through miR-142-5p/β-catenin by Yukai Dai, Qizhong Xu, Manqi Xia, Caimin Chen, Xinming Xiong, Xin Yang, Wei Wang

“…Methods Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to validate the expression of hsa_circ_0001615 and miR-142-5p. …”
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Expression of CD44 standard form and variant isoforms in human bone marrow stromal cells by Xiaofeng Wang, Zhenwu Du, Xiaojun Liu, Yang Song, Guizhen Zhang, Zhitao Wang, Qingyu Wang, Zhongli Gao, Yajun Wang, Wei Wang

“…The expressions of CD44S and CD44V were examined using quantitative real-time polymerase chain reaction (q-PCR). Results showed that hBMSCs were successfully cultured, with positive expressions of markers of mesenchymal cells (CD90, CD73, CD105), and negative expressions of markers of hematopoietic cells (CD34, CD45). …”
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Overexpressed GATA3 enhances the sensitivity of colorectal cancer cells to oxaliplatin through regulating MiR-29b by Wei Wang, Mei Wang, Jing Xu, Fei Long, Xianbao Zhan

“…The expression levels of GATA3, caspase3 and cleaved caspase3 were determined by Western blot, and the expression of miR-29b was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Animal experiments were performed to examine the changes of transplanted tumors in nude mouse xenograft studies and observed by in vivo imaging. …”
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Netrin-1 Role in Nociceptive Neuron Sprouting through Activation of DCC Signaling in a Rat Model of Bone Cancer Pain by Zhihao Gong, Yuxin Zhang, Wei Wang, Xin Li, Kai Wang, Xingji You, Jingxiang Wu

“…Expressions of associated molecules in the affected tibias or dorsal root ganglia (DRG) were measured by immunofluorescence, immunohistochemistry, real-time quantitative polymerase chain reaction, or western blotting. Transduction of deleted in colorectal cancer (DCC) signaling was inhibited by intrathecal injection of DCC-siRNA. …”
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Apol9a regulates myogenic differentiation via the ERK1/2 pathway in C2C12 cells by Xuan Jiang, Xuan Jiang, Siyu Ji, Siyuan Cui, Rong Wang, Rong Wang, Wei Wang, Yongquan Chen, Yongquan Chen, Yongquan Chen, Shenglong Zhu, Shenglong Zhu

“…C2C12 cells were used to assess the myogenic effect of Apol9a through immunocytochemistry, western blotting, quantitative polymerase chain reaction, RNA interference or overexpression, and lipidomics.Results: RNA-seq of differentiated and undifferentiated C2C12 cells revealed that Apol9a expression significantly increased following myogenic differentiation and decreased during obesity-induced muscle atrophy. …”
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Deciphering the role of extrachromosomal circular DNA in adipose stem cells from old and young donors by Sen Ren, Du Wu, Xiaoyong Shen, Qian Wu, Chengcheng Li, Hewei Xiong, Zhongwei Xiong, Rui Gong, Zheng Liu, Wei Wang, Jincao Chen

“…The differentially expressed eccDNAs were calculated, annotated and validated via polymerase chain reaction. Results Thousands of eccDNAs were identified and comprehensively characterized. …”
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miR‐29a‐3p promotes the regulatory role of eicosapentaenoic acid in the NLRP3 inflammasome and autophagy in microglial cells by Jian‐Ping Pan, Jia‐Li Xie, Li‐Yun Huang, Qi‐Zhen Wu, Dan‐Feng Tang, Qi Jin, Wei Wang, Ming‐Fu Yang

“…Methylated miR‐29a‐3p was detected via methylation‐specific polymerase chain reaction. EPA treatment at 60 μM had no cytotoxic effects on BV2 cells and significantly inhibited the LPS‐induced inflammatory response and NLRP3 inflammasome but activated autophagy, while all these effects were reversed by the autophagy inhibitor 3‐MA. …”
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FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic Protein by Jiaojiao Yang, Juanli Zhang, Xiaoli Gao, Ruirui Luo, Kaihui Xie, Wei Wang, Jie Li, Qiaoli Yang, Xiaoyu Huang, Zunqiang Yan, Pengfei Wang, Shuangbao Gun

“…Here, an in vitro model of CPTCIPD was constructed by treating the intestinal porcine epithelial cell line-J2 (IPEC-J2) with <i>Clostridium perfringens</i> beta2 (CPB2) toxin, and the role of FTO was analyzed using quantitative real-time polymerase chain reaction, Western blotting, and flow cytometry. …”
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Prognostic value of plasma microRNAs for non-small cell lung cancer based on data mining models by Mengqing Yan, Wenjun Kang, Xiaohua Liu, Bin Yang, Na Sun, Yongli Yang, Wei Wang

“…The expressions of plasma miRNAs were examined by quantitative polymerase chain reaction (qPCR). The Whitney U test was used to analyze non-normally distributed data. …”
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CD27-Expressing Xenoantigen-Expanded Human Regulatory T Cells Are Efficient in Suppressing Xenogeneic Immune Response by Lu Cao, Xiaoqian Ma, Juan Zhang, Min Yang, Zhenhu He, Cejun Yang, Sang Li, Pengfei Rong, Wei Wang

“…We enriched CD27 + xeno-Tregs using cell sorting and evaluated their suppressive functions and stability in vitro via mixed lymphocyte reaction (MLR), real-time polymerase chain reaction, inflammatory induction assay, and Western blotting. …”
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