Effects of high‐fat diet on nutrient metabolism and cognitive functions in young APPKINL‐G‐F/NL‐G‐F mice by Wei Wang, Daisuke Tanokashira, Yudai Shibayama, Ryuhei Tsuji, Megumi Maruyama, Chiemi Kuroiwa, Takashi Saito, Takaomi C. Saido, Akiko Taguchi

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Genetic Interaction of <i>Thm2</i> and <i>Thm1</i> Shapes Postnatal Craniofacial Bone by Erin E. Bumann, Portia Hahn Leat, Henry H. Wang, Brittany M. Hufft-Martinez, Wei Wang, Pamela V. Tran

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Secreted protein acidic and rich in cysteine (SPARC) suppresses angiogenesis by down-regulating the expression of VEGF and MMP-7 in gastric cancer. by Jun-Ling Zhang, Guo-Wei Chen, Yu-Cun Liu, Peng-Yuan Wang, Xin Wang, Yuan-Lian Wan, Jing Zhu, Hong-Qiao Gao, Jie Yin, Wei Wang, Mao-Lin Tian

“…Elevated angiogenesis induced by SPARC silencing in HGC-sh cells was decreased when VEGF was neutralised by antibodies, and MMP-7 was knocked down in vitro. CONCLUSION: SPARC suppresses angiogenesis of gastric cancer by down-regulating the expression of VEGF and MMP-7.…”
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BMP9 inhibits proliferation and metastasis of HER2-positive SK-BR-3 breast cancer cells through ERK1/2 and PI3K/AKT pathways. by Wei Ren, Yuehong Liu, Shaoheng Wan, Chang Fei, Wei Wang, Yingying Chen, Zhihui Zhang, Ting Wang, Jinshu Wang, Lan Zhou, Yaguang Weng, Tongchuan He, Yan Zhang

“…In SK-BR-3 cells with BMP9 over-expression, the phosphorylation of HER2, ERK1/2 and AKT was markedly suppressed and the HER2 expression decreased at both mRNA and protein levels, while opposite results were observed in SK-BR-3 cells with BMP9 knock down. When the phosphorylation of ERK1/2 and PI3K/AKT was inhibited by PD98059 and LY294002, respectively, the decreased proliferation and invasion induced by BMP9 knock down were eliminated. …”
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MYBL2 regulates de novo purine synthesis by transcriptionally activating IMPDH1 in hepatocellular carcinoma cells by Jun-Zhang Zhao, Wei Wang, Tao Liu, Lei Zhang, De-Zheng Lin, Jia-Yin Yao, Xiang Peng, Gang Jin, Tian-Tian Ma, Jin-Bo Gao, Fang Huang, Jun Nie, Qing Lv

“…Metabolomic analysis post knocking-out of MYBL2 demonstrated that it was essential in de novo purine synthesis, especially guanine nucleotides. …”
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A Specific Mini‐Intrabody Mediates Lysosome Degradation of Mutant Huntingtin by Caijuan Li, Yingqi Lin, Yizhi Chen, Xichen Song, Xiao Zheng, Jiawei Li, Jun He, Xiusheng Chen, Chunhui Huang, Wei Wang, Jianhao Wu, Jiaxi Wu, Jiale Gao, Zhuchi Tu, Xiao‐Jiang Li, Sen Yan, Shihua Li

“…It is found that delivery of this peptide by either brain injection or intravenous administration can efficiently clear the soluble and aggregated mHTT by activating the lysosomal degradation pathway, resulting in amelioration of gliosis and dyskinesia in HD knock‐in (KI‐140Q) mice. These findings suggest that the small intrabody peptide linked to lysosomes can effectively lower mutant proteins and provide a new approach for treating neurodegenerative diseases that are caused by the accumulation of mutant proteins.…”
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MTFR2 Promotes the Proliferation, Migration, and Invasion of Oral Squamous Carcinoma by Switching OXPHOS to Glycolysis by Wei Wang, Meihua Xiong, Lin Jiang, Zean Chen, Yisen Shao

“…We next established stable MTFR2-overexpressing and MTFR2 knocking down cell lines. A series of experiments were applied and the results indicated that MTFR2 was upregulated in cancer tissue and negatively correlated with the overall survival (OS) of patients in both the TCGA database and our inhouse database. …”
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Identification of the <i>CKM</i> Gene as a Potential Muscle-Specific Safe Harbor Locus in Pig Genome by Youcai Xiong, Rongzhi Zhuang, Guangxing Zhao, Yanwen Liu, Yinyu Su, Wei Wang, Xiaoning Xi, Yanyu Yang, Xiaosong Han, Shengsong Xie, Heng Wang, Xinyun Li, Bo Zuo, Shuhong Zhao, Zheng Feng, Jinxue Ruan

“…Here, we use CRISPR/Cas9-mediated homologous directed repair (HDR) to accurately knock the exogenous gene-of-interest fragments into an endogenous <i>CKM</i> gene in the porcine satellite cells. …”
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Upregulation of LPGAT1 Enhances Lung Adenocarcinoma Proliferation by Huiyuan Gong, Chao Ma, Xiaojun Li, Xueying Zhang, Linxiang Zhang, Pengfei Chen, Wei Wang, Yannan Hu, Ting Huang, Nan Wu, Xiaojing Wang

“…The effect of LPGAT1 was then assessed in both cell and animal models after LPGAT1 was knocked down by RNA interference. Results: LPGAT1 was upregulated in LUAD tissues. …”
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Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis by Suhui Zhang, Yingqian Zhang, Peng Zhang, Zechen Wei, Mingrui Ma, Wei Wang, Wei Tong, Feng Tian, Hui Hui, Jie Tian, Yundai Chen

“…We found that oscillatory shear induced the increase of PLXND1 in M1 macrophages, and knocking down PLXND1 inhibited M1 polarization. Semaphorin 3E, the ligand of PLXND1 which was highly expressed in plaques, strongly enhanced M1 macrophage polarization via PLXND1 in vitro. …”
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Immune suppressive signaling regulated by latent transforming growth factor beta binding protein 1 promotes metastasis in cervical cancer by Haiyan Gu, Wei Wang, Changdong Sun, Li Ding, Li Li, Peng Shu, Jun Xu

“…Cell proliferation, cell cycle, migration, and in vivo metastasis were determined after LTBP1 was knocked down. Then, 13 drugs were screened, and the changes in cell apoptosis and proliferation and tumor metastasis were detected after drug treatment in shRNA cells. …”
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Single-cell transcriptome analysis of epithelial, immune, and stromal signatures and interactions in human ovarian cancer by Chaochao Chai, Langchao Liang, Nanna S. Mikkelsen, Wei Wang, Wandong Zhao, Chengcheng Sun, Rasmus O. Bak, Hanbo Li, Lin Lin, Fei Wang, Yonglun Luo

“…Furthermore, the NECTIN2-TIGIT ligand-receptor pair was identified to mediate T cells communicating with epithelial, fibroblast, endothelial, and other cell types. Knock-out of NECTIN2 using CRISPR/Cas9 inhibited ovarian cancer cell (SKOV3) proliferation, and increased T cell proliferation when co-cultured. …”
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<i>OsExo70B1</i> Positively Regulates Disease Resistance to <i>Magnaporthe oryzae</i> in Rice by Hongna Hou, Jianbo Fang, Jiahui Liang, Zhijuan Diao, Wei Wang, Dewei Yang, Shengping Li, Dingzhong Tang

“…<i>OsExo70B1</i> mainly expresses in leaf and shoot and its expression is induced by pathogen-associated molecular patterns (PAMPs) and rice blast fungus <i>Magnaporthe oryzae</i> (<i>M. oryzae</i>). Knocking out <i>OsExo70B1</i> results in significantly decreased resistance and defense responses to <i>M. oryzae</i> compared to the wild type, including more disease lesions and enhanced fungal growth, downregulated expression of pathogenesis-related (PR) genes, and decreased reactive oxygen species accumulation. …”
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Regulation of RNA Interference Pathways in the Insect Vector <i>Laodelphax striatellus</i> by Viral Proteins of Rice Stripe Virus by Yan Xiao, Qiong Li, Wei Wang, Yumei Fu, Feng Cui

“…Protein-protein binding assays showed that viral NS2 and RNA-dependent RNA polymerase interacted with insect Ago2 and Translin, respectively. When <i>NS2</i> was knocked down, the transcript level of <i>Ago2</i> increased and viral replication was inhibited. …”
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Chalcone Derivative CX258 Suppresses Colorectal Cancer via Inhibiting the TOP2A/Wnt/β-Catenin Signaling by Xi Chen, Xiaocheng Lv, Lijie Gao, Jiawei Liu, Wei Wang, Lichao Guo, Mykhaylo S. Frasinyuk, Wen Zhang, David S. Watt, Chunming Liu, Xifu Liu

“…Additionally, CX258 reduced the expression of DNA Topoisomerase II alpha (TOP2A) in CRC cells. Moreover, knocking down TOP2A by siRNAs inhibited the Wnt/β-catenin signaling pathway, a finding suggesting that CX258 inhibited Wnt/β-catenin signaling and CRC cell proliferation at least partially by modulating TOP2A. …”
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Deletion of SM22α disrupts the structure and function of caveolae and T-tubules in cardiomyocytes, contributing to heart failure. by Jun Wu, Wei Wang, Yaomeng Huang, Haochen Wu, Jiabin Wang, Mei Han

“…<h4>Methods</h4>SM22α knock-out (KO) mice were utilized to assess the role of SM22α in the heart. …”
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Overexpressed GATA3 enhances the sensitivity of colorectal cancer cells to oxaliplatin through regulating MiR-29b by Wei Wang, Mei Wang, Jing Xu, Fei Long, Xianbao Zhan

“…TUNEL staining results showed that knocking down or overexpression of GATA3 did not cause significant changes to apoptotic bodies of CRC cells, while oxaliplatin treatment increased the number of apoptotic bodies. …”
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Genome Editing of Rice <i>eIF4G</i> Loci Confers Partial Resistance to Rice Black-Streaked Dwarf Virus by Wei Wang, Shuhui Ma, Peng Hu, Yinghua Ji, Feng Sun

“…Furthermore, our data suggest that <i>eIF4G</i> may plays an important role in rice normal development, as there were no <i>eIF4G</i> knock-out homozygous mutants in T1 generation plants. …”
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Aberrant palmitoylation caused by a ZDHHC21 mutation contributes to pathophysiology of Alzheimer’s disease by Wenwen Li, Yana Pang, Yan Wang, Fan Mei, Mengmeng Guo, Yiping Wei, Xinyue Li, Wei Qin, Wei Wang, Longfei Jia, Jianping Jia

“…Methods We performed exome sequencing on family members with a FAD pedigree and identified gene variant ZDHHC21 p.T209S. A ZDHHC21T209S/T209S knock-in mouse model was then generated using CRISPR/Cas9. …”
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