Highly efficient generation of biallelic reporter gene knock-in mice via CRISPR-mediated genome editing of ESCs by Yanliang Wang, Junhong Li, Jinzhu Xiang, Bingqiang Wen, Haiyuan Mu, Wei Zhang, Jianyong Han

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Erratum to: Highly efficient generation of biallelic reporter gene knock-in mice via CRISPR-mediated genome editing of ESCs by Yanliang Wang, Junhong Li, Jinzhu Xiang, Bingqiang Wen, Haiyuan Mu, Wei Zhang, Jianyong Han

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Particle-Size-Dependent Delivery of Antitumoral miRNA Using Targeted Mesoporous Silica Nanoparticles by Lisa Haddick, Wei Zhang, Sören Reinhard, Karin Möller, Hanna Engelke, Ernst Wagner, Thomas Bein

“…We demonstrate that nanoparticles with a diameter of 160 nm show the fastest cellular internalization after a very short incubation time of 45 min and produce the highest level of gene knock-down.…”
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CircRAPGEF5 interacts with RBFOX2 to confer ferroptosis resistance by modulating alternative splicing of TFRC in endometrial cancer by Jun Zhang, Shuaijun Chen, Sitian Wei, Shuangshuang Cheng, Rui Shi, Rong Zhao, Wei Zhang, Qi Zhang, Teng Hua, Dilu Feng, Zhicheng Yu, Hongbo Wang

“…Additionally, a series of gain- and loss-of-function experiments demonstrated that knocking down or overexpressing RBFOX2 reversed the effects of knocking down or overexpressing circRAPGEF5 in EC cells. …”
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Baicalin suppresses colorectal cancer cell proliferation, potentially via ARRDC4: Bioinformatics and experimental analysis by Shuai Yan, Yahui Wang, Yunhui Gu, Mingyue Zhou, Lianlin Su, Tianpeng Yin, Wei Zhang, Yinzi Yue

“…ARRDC4 expression was knocked down by transfecting CRC cells with lentiviral shRNA. …”
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CircHIPK3 promotes proliferation and metastasis and inhibits apoptosis of renal cancer cells by inhibiting MiR-485-3p by Jinjin Lai, Jun Xin, Changde Fu, Wei Zhang

“…Results CircHIPK3 was mainly overexpressed in the cytoplasm of RC tissues and cells. Knocking down circHIPK3 inhibited the proliferation, migration, and invasion of RC cells. …”
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Evaluation of safety and immunogenicity of duck-plague virus gC/gE double gene deletion by Peilin Ruan, Peilin Ruan, Peilin Ruan, Xin Feng, Xin Feng, Xin Feng, Anchun Cheng, Anchun Cheng, Anchun Cheng, Mingshu Wang, Mingshu Wang, Mingshu Wang, Wei Zhang, Ying Wu, Ying Wu, Ying Wu, Qiao Yang, Qiao Yang, Qiao Yang, Bin Tian, Bin Tian, Bin Tian, Xuming Ou, Xuming Ou, Xuming Ou, Di Sun, Di Sun, Di Sun, Shaqiu Zhang, Shaqiu Zhang, Shaqiu Zhang, Sai Mao, Sai Mao, Sai Mao, Dekang Zhu, Dekang Zhu, Renyong Jia, Renyong Jia, Renyong Jia, Shun Chen, Shun Chen, Shun Chen, Mafeng Liu, Mafeng Liu, Mafeng Liu, Xin-Xin Zhao, Xin-Xin Zhao, Xin-Xin Zhao, Juan Huang, Juan Huang, Juan Huang, Qun Gao, Qun Gao, Qun Gao, Yanling Yu, Yanling Yu, Yanling Yu, Ling Zhang, Ling Zhang, Ling Zhang, Leichang Pan, Leichang Pan

“…In this study, on the basis of the duck plague virus gC gene deletion strain CHv-ΔgC, based on the duck plague virus bacterial artificial chromosome (BAC) platform in our laboratory, the gE gene was knocked out using the traceless deletion technology to obtain gC/gE double gene deletion candidate vaccine strain CHv-ΔgC/gE. …”
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Immunogenic cell death mediation patterns reveal novel paradigm for characterizing the immune microenvironment and immunotherapeutic responses in bladder cancer by Jialei Fu, Wei Zhang, Tao Jiang

“…The ICD-related gene, CALR, was significantly upregulated in the T24 BCA cell line relative to the control SV-HUC-1 cells. Knocking down CALR suppressed T24 cell viability and caused ER stress.Conclusion: We identified the existence of distinct ICD-mediated patterns in BCA closely associated with the remodeling of the TIME. …”
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PARP14 inhibition restores PD-1 immune checkpoint inhibitor response following IFNγ-driven acquired resistance in preclinical cancer models by Chun Wai Wong, Christos Evangelou, Kieran N. Sefton, Rotem Leshem, Wei Zhang, Vishaka Gopalan, Sorayut Chattrakarn, Macarena Lucia Fernandez Carro, Erez Uzuner, Holly Mole, Daniel J. Wilcock, Michael P. Smith, Kleita Sergiou, Brian A. Telfer, Dervla T. Isaac, Chang Liu, Nicholas R. Perl, Kerrie Marie, Paul Lorigan, Kaye J. Williams, Patricia E. Rao, Raghavendar T. Nagaraju, Mario Niepel, Adam F. L. Hurlstone

“…Effector T cell infiltration is enhanced in tumours derived from cells pre-treated with IFNγ in immunocompetent female mice when PARP14 is pharmacologically inhibited or knocked down, while the presence of regulatory T cells is decreased, leading to restoration of α-PD-1 sensitivity. …”
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ZC3H4 regulates infiltrating monocytes, attenuating pulmonary fibrosis through IL-10 by Yaping Liu, Xinxin Zhang, Jing Wang, Fuhuang Yang, Wei Luo, Jie Huang, Mengling Chen, Sha Wang, Caolong Li, Wei Zhang, Jie Chao

“…The CRISPR/Cas9 system was used to specifically knock down ZC3H4, a novel member of the CCCH zinc finger protein family, and was combined with pharmacological methods to explore the mechanism by which ZC3H4 affects chemokine and cytokine secretion. …”
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Overexpression of PKMYT1 associated with poor prognosis and immune infiltration may serve as a target in triple-negative breast cancer by Huihui Li, Li Wang, Wei Zhang, Youting Dong, Yefeng Cai, Yefeng Cai, Xiaoli Huang, Xiaoli Huang, Xubin Dong

“…TNBC cell lines’ proliferation, migration, and invasion were inhibited when PKMYT1 expression was knock-down. Besides, the down-regulation of PKMYT1 induced apoptosis in vitro. …”
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Long non-coding RNA TTN antisense RNA 1 facilitates hepatocellular carcinoma progression via regulating miR-139-5p/SPOCK1 axis by Xinghao Zhu, Shiqing Jiang, Zongyao Wu, Tonghua Liu, Wei Zhang, Lili Wu, Lijun Xu, Mingliang Shao

“…After TTN-AS1 was overexpressed or knocked down in HCC cells, CCK-8 and 5-Ethynyl-2ʹ-deoxyuridine (EdU) assays were carried out for examining cell multiplication. …”
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OTUB2 Regulates YAP1/TAZ to Promotes the Progression of Esophageal Squamous Cell Carcinoma by Li Liu, Hu Cheng, Min Ji, Liping Su, Ziyang Lu, Xiayun Hu, Yaling Guan, Jinling Xiao, Lijuan Ma, Wei Zhang, Hongwei Pu

“…The protein expression levels of YAP1, TAZ and CTGF decreased after knocking down the expression of OTUB2 (P < 0.05). OTUB2 knockdown in ESCC cell lines suppressed YAP1/TAZ signaling. …”
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IRF1 governs the expression of SMARCC1 via the GCN5-SETD2 axis and actively engages in the advancement of osteoarthritis by Dong Wang, Yujun Zhang, Liangping Zhang, Du He, Lan Zhao, Zhimin Miao, Wei Cheng, Chengyue Zhu, Li Zhu, Wei Zhang, Hongting Jin, Hang Zhu, Hao Pan

“…The Translational Potential of this Article: The study demonstrated that the regulation of SMARCC1 by IRF1 plays a crucial role in the development of OA. Knocking down either IRF1 or SMARCC1 disrupted the pro-inflammatory effects induced by IL-1β in chondrocytes, leading to a mitigation of OA-like symptoms in rat models. …”
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