The schizophrenia-associated missense variant rs13107325 regulates dendritic spine density by Shiwu Li, Changguo Ma, Yifan Li, Rui Chen, Yixing Liu, Li Pear Wan, Qiuxia Xiong, Chuang Wang, Yongxia Huo, Xinglun Dang, Yongfeng Yang, Luxian Lv, Xi Chen, Nengyin Sheng, Wenqiang Li, Xiong-Jian Luo

“…Many of the tested phenotypes did not show differences in SLC39A8-p.393T knock-in and wild-type mice. However, we found that zinc concentration in brain and blood of SLC39A8-p.393T knock-in mice was dysregulated compared with wild-types, validating the functionality of rs13107325. …”
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ALKBH5 Stabilized N⁶-Methyladenosine—Modified LOC4191 to Suppress <i>E. coli</i>-Induced Apoptosis by Haojun Xu, Changjie Lin, Chao Wang, Tianrui Zhao, Jinghan Yang, Junhao Zhang, Yanjun Hu, Xue Qi, Xi Chen, Yingyu Chen, Jianguo Chen, Aizhen Guo, Changmin Hu

“…Meanwhile, knocking down ALKBH5 resulted in the promotion of apoptosis through upregulated ROS and arrested the cell cycle in MAC-T cells. …”
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Reprogramming Mycobacterium tuberculosis CRISPR System for Gene Editing and Genome-wide RNA Interference Screening by Khaista Rahman, Muhammad Jamal, Xi Chen, Wei Zhou, Bin Yang, Yanyan Zou, Weize Xu, Yingying Lei, Chengchao Wu, Xiaojian Cao, Rohit Tyagi, Muhammad Ahsan Naeem, Da Lin, Zeshan Habib, Nan Peng, Zhen F. Fu, Gang Cao

“…We demonstrated that M. tuberculosis genes can be efficiently and specifically knocked in/out by this system as confirmed by DNA high-throughput sequencing. …”
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Dgcr8 deletion in the primitive heart uncovered novel microRNA regulating the balance of cardiac-vascular gene program by Xi Chen, Lin Wang, Rujin Huang, Hui Qiu, Peizhe Wang, Daren Wu, Yonglin Zhu, Jia Ming, Yangming Wang, Jianbin Wang, Jie Na

“…Abstract Primitive mammalian heart transforms from a single tube to a four-chambered muscular organ during a short developmental window. We found that knocking out global microRNA by deleting Dgcr8 microprocessor in Mesp1 cardiovascular progenitor cells lead to the formation of extremely dilated and enlarged heart due to defective cardiomyocyte (CM) differentiation. …”
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miR-10a inhibits cell proliferation and promotes cell apoptosis by targeting BCL6 in diffuse large B-cell lymphoma by Qian Fan, Xiangrui Meng, Hongwei Liang, Huilai Zhang, Xianming Liu, Lanfang Li, Wei Li, Wu Sun, Haiyang Zhang, Ke Zen, Chen-Yu Zhang, Zhen Zhou, Xi Chen, Yi Ba

“…By overexpressing or knocking down miR-10a in DLBCL cells, we experimentally validated that miR-10a directly recognizes the 3′-UTR of the BCL6 transcript and regulated BCL6 expression. …”
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lncRNA JPX modulates malignant progress of osteosarcoma through targeting miR-33a-5p and PNMA1 regulatory loop by Wei Xiong, Dan Liu, Xi Chen, Leiting Liu, Weihong Xiao

“…We subsequently examined the functional role of JPX in OS cells through knocked-down JPX by using siRNA. JPX down-regulation was observed to suppress OS cell proliferation, migration and invasion. …”
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USP53 plays an antitumor role in hepatocellular carcinoma through deubiquitination of cytochrome c by Ye Yao, Weijie Ma, Yonghua Guo, Yingyi Liu, Peng Xia, Xiaoling Wu, Yiran Chen, Kunlei Wang, Chengjie Mei, Ganggang Wang, Xiaomian Li, Zhonglin Zhang, Xi Chen, Yufeng Yuan

“…Finally, the overexpression of CYCS compensated for the decreased apoptotic rates in cells with USP53 knocked down, suggesting that USP53 induced the apoptosis in HCC cells through the deubiquitination of CYCS. …”
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Chalcone Derivative CX258 Suppresses Colorectal Cancer via Inhibiting the TOP2A/Wnt/β-Catenin Signaling by Xi Chen, Xiaocheng Lv, Lijie Gao, Jiawei Liu, Wei Wang, Lichao Guo, Mykhaylo S. Frasinyuk, Wen Zhang, David S. Watt, Chunming Liu, Xifu Liu

“…Additionally, CX258 reduced the expression of DNA Topoisomerase II alpha (TOP2A) in CRC cells. Moreover, knocking down TOP2A by siRNAs inhibited the Wnt/β-catenin signaling pathway, a finding suggesting that CX258 inhibited Wnt/β-catenin signaling and CRC cell proliferation at least partially by modulating TOP2A. …”
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CD200 in dentate gyrus improves depressive-like behaviors of mice through enhancing hippocampal neurogenesis via alleviation of microglia hyperactivation by Xi Chen, Qian-Qian Cui, Xiao-Hai Hu, Jian Ye, Zi-Cun Liu, Yuan-Xi Mei, Fang Wang, Zhuang-Li Hu, Jian-Guo Chen

“…When CD200R1 receptors on microglia were knocked down, CD200 was unable to exert its role in alleviating depressive-like behavior. …”
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Effect of low expression of senescence marker protein 30 on proliferation and oxidation of human lens epithelial cells line SRA01/04 under high calcium conditions by Song-Man Li, Zi-Hao Han, Aint Thu Thu Win, Xi Chen, Hao Liang

“…<p>METHODS: Three RNAi sequences were designed to knock down SMP30 target gene RGN expression(KD1-3), and the blank-load sequence was used as the negative control group(NCKD), all of which were used to construct lentiviral vectors to infect SRA01/04 cells. …”
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The role of Nurr1-miR-30e-5p-NLRP3 axis in inflammation-mediated neurodegeneration: insights from mouse models and patients’ studies in Parkinson’s disease by Tianbai Li, Xiang Tan, Lulu Tian, Congcong Jia, Cheng Cheng, Xi Chen, Min Wei, Yuanyuan Wang, Yiying Hu, Qiqi Jia, Yang Ni, Murad Al-Nusaif, Song Li, Weidong Le

“…To further explore the involvement of Nurr1-miR-30e-5p-NLRP3 axis in the inflammation-mediated PD pathology, we developed a mouse model (Nurr1flox+/Cd11b−cre+, Nurr1cKO) conditionally knocking out Nurr1 in Cd11b-expressing cells. Our investigations in Nurr1cKO mice unveiled significant dopaminergic neurodegeneration following lipopolysaccharide-induced inflammation. …”
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