Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method by Changqin Li, Yiping Cui, Jie Lu, Lijun Meng, Changyang Ma, Zhenhua Liu, Yan Zhang, Wenyi Kang

Subjects: Get full text

Spectrum Effect Relationship and Component Knock-Out in Angelica Dahurica Radix by High Performance Liquid Chromatography-Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer by Jinmei Wang, Linna Peng, Mengjun Shi, Changqin Li, Yan Zhang, Wenyi Kang

Subjects: Get full text

Maternal RNA transcription in Dlk1-Dio3 domain is critical for proper development of the mouse placental vasculature by Ximeijia Zhang, Hongjuan He, Haoran Yu, Xiangqi Teng, Ziwen Wang, Chenghao Li, Jiahang Li, Haopeng Yang, Jiwei Shen, Tong Wu, Fengwei Zhang, Yan Zhang, Qiong Wu

“…PolyA signals were inserted into Gtl2 to establish a mouse model to prevent the expression of maternal RNAs in the domain. The maternal allele knock-in (MKI) and homozygous (HOMO) placentas showed an expanded junctional zone, reduced labyrinth and poor vasculature impacting both fetal and maternal blood spaces. …”
Get full text

BMP9 inhibits proliferation and metastasis of HER2-positive SK-BR-3 breast cancer cells through ERK1/2 and PI3K/AKT pathways. by Wei Ren, Yuehong Liu, Shaoheng Wan, Chang Fei, Wei Wang, Yingying Chen, Zhihui Zhang, Ting Wang, Jinshu Wang, Lan Zhou, Yaguang Weng, Tongchuan He, Yan Zhang

“…In SK-BR-3 cells with BMP9 over-expression, the phosphorylation of HER2, ERK1/2 and AKT was markedly suppressed and the HER2 expression decreased at both mRNA and protein levels, while opposite results were observed in SK-BR-3 cells with BMP9 knock down. When the phosphorylation of ERK1/2 and PI3K/AKT was inhibited by PD98059 and LY294002, respectively, the decreased proliferation and invasion induced by BMP9 knock down were eliminated. …”
Get full text

Role of skeletal muscle satellite cells in the repair of osteoporotic fractures mediated by β‐catenin by Zhenxiong Jin, Weiwei Da, Yongjian Zhao, Tengteng Wang, Hao Xu, Bing Shu, Xiang Gao, Qi Shi, Yong Ma, Yan Zhang, Yongjun Wang, Dezhi Tang

“…To further clarify the scientific hypothesis, we innovatively used Pax7‐CreERT2/+;β‐cateninfx/fx transgenic mice (n = 12, per group, male). Knock out β‐catenin in SMSC to observe the proliferation and osteogenic differentiation of SMSCs, and OPF healing. …”
Get full text

Postsynaptic histamine H3 receptors in ventral basal forebrain cholinergic neurons modulate contextual fear memory by Yanrong Zheng, Lishi Fan, Zhuowen Fang, Zonghan Liu, Jiahui Chen, Xiangnan Zhang, Yi Wang, Yan Zhang, Lei Jiang, Zhong Chen, Weiwei Hu

“…Consistently, genetically knocking down H3R or chemogenetically activating cholinergic neurons in the ventral basal forebrain (vBF) mimics this enhanced fear memory, whereas the freezing augmentation is rescued by re-expressing H3R or chemogenetic inhibition of vBF cholinergic neurons. …”
Get full text

Odorant Receptor OR2C1 Is an Essential Modulator of Boar Sperm Capacitation by Binding with Heparin by Xiang Yuan, Yihan Wang, Malik Ahsan Ali, Ziyue Qin, Zhihua Guo, Yan Zhang, Ming Zhang, Guangbin Zhou, Jiandong Yang, Lei Chen, Linyuan Shen, Li Zhu, Changjun Zeng

“…Furthermore, we demonstrated that knocking down <i>OR2C1</i> significantly inhibits sperm capacitation. …”
Get full text

A hybrid nanoassembly for ultrasound-inducible cytosolic siRNA delivery and cancer sono-gene therapy by Gaigai Li, Yan Zhang, Jinbo Li

“…After cellular uptake and exposure to sono-irradiation, HNA generated singlet oxygen to facilitate the lysosomal escape of siRNA to knock down anti-apoptotic Bcl-2 in the cytoplasm. …”
Get full text

ATF3 promotes ferroptosis in sorafenib-induced cardiotoxicity by suppressing Slc7a11 expression by Yilan Li, Yilan Li, Jingru Yan, Jingru Yan, Qianqian Zhao, Qianqian Zhao, Yan Zhang, Yao Zhang, Yao Zhang

“…Overexpression of Slc7a11 protected cells from ferroptosis, while knock-down of Slc7a11 made cardiomyoblasts sensitive to ferroptosis caused by sorafenib. …”
Get full text

Using <i>Staphylococcus aureus</i> Cas9 to Expand the Scope of Potential Gene Targets for Genome Editing in Soybean by Yan Zhang, Yupeng Cai, Shi Sun, Tianfu Han, Li Chen, Wensheng Hou

“…The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) is a revolutionary genome editing technology that has been used to achieve site-specific gene knock-out, large fragment deletion, or base editing in many plant species including soybean (<i>Glycine</i><i>max</i>). …”
Get full text

EEF1A1 is Involved the Regulating Neuroinflammatory Processes in Parkinson's Disease by Zaolaguli Aisha, Jing Lei, Yan Zhang, Jianhua Ma

“…Our study’s primary objective was to knock down EEF1A1 in U251 cells and preliminarily explore the role of EEF1A1 in PD neuroinflammation. …”
Get full text

MiR-196b-5p activates NF-κB signaling in non-small cell lung cancer by directly targeting NFKBIA by Wangyu Zhu, Yun Yu, Yuxin Ye, Xinyue Tu, Yan Zhang, Tao Wu, Lianli Ni, Xiangjie Huang, Yumin Wang, Ri Cui

“…Results: We demonstrated that the miR-196b-5p facilitates lung cancer cell proliferation, migration, colony formation, and cell cycle by directly targeting NFKBIA, a negative regulator of NFκB signaling. Knocking down NFKBIA increases IL6 mediated phosphorylation of signal transducer and activator of transcription 3 (STAT3) to promote lung cancer cell growth by activating NFκB signaling. …”
Get full text

Development and implementation of a Type I-C CRISPR-based programmable repression system for Neisseria gonorrhoeae by Wendy E. Geslewitz, Amaris Cardenas, Xufei Zhou, Yan Zhang, Alison K. Criss, H Steven Seifert

“…Importantly, we also show that we can use CRISPRi to knockdown genes that are essential to Gc that cannot normally be knocked out under laboratory settings. Gc encodes ~800 essential genes, many of which have no predicted function. …”
Get full text