High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants by Huaping Zeng, Kexin Chen, Chouxian Ma, Biyin Zhu, Jun Chuan, Shuan Zhang, Lin Tang, Ting Yang, Zhaohui Sun, Xingkun Yang, Yu Wang

“…Abstract Polymerase chain reaction (PCR) variants requiring specific primer types are widely used in various PCR experiments, including generic PCR, inverse PCR, anchored PCR, and ARMS PCR. …”
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Spatial tumor biopsy with fluorescence PCR microneedle array by Xiaoxuan Zhang, Guopu Chen, Yu Wang, Yuanjin Zhao

“…Resembling the discrete wettability of desert beetles, in this study, a fluorescence polymerase chain reaction (F-PCR) microneedle array (MNA) platform is developed for efficient spatial tumor biopsy. …”
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RNA-seq reveals Nup62 as a potential regulator for cell division after traumatic brain injury in mice hippocampus by Jianwei Zhao, Weihua Wang, Ke Yan, Haifeng Zhao, Zhen Zhang, Yu Wang, Wenyu Zhu, Shiwen Chen

“…Real-time quantitative polymerase chain reaction and western blot were adopted to verify the sequencing results. …”
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Identification of key biomarkers based on the proliferation of secondary hyperparathyroidism by bioinformatics analysis and machine learning by Aiwen Shen, Jialin Shi, Yu Wang, Qian Zhang, Jing Chen

“…Immunohistochemistry (IHC) staining and quantitative real-time polymerase chain reaction (qRT-PCR) were used to verify the expression of screened genes in parathyroid tissues of SHPT patients and animal models. …”
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Genome-wide identification of the CYP82 gene family in cucumber and functional characterization of CsCYP82D102 in regulating resistance to powdery mildew by Hongyu Wang, Pengfei Li, Yu Wang, Chunyu Chi, Guohua Ding

“…Expression patterns of the CYP82 genes were characterized by mining available RNA-seq data followed by qRT-PCR (quantitative real-time polymerase chain reaction) analysis. Members of CYP82 genes display specific expression profiles in different tissues, and in response to PM and abiotic stresses in this study, the role of CsCYP82D102, a member of the CYP82 gene family, was investigated. …”
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Functional, morphological and molecular characteristics in a novel rat model of spinal sacral nerve injury-surgical approach, pathological process and clinical relevance by Junyang Li, Shiqiang Li, Yu Wang, Aijia Shang

“…Immunostaining of nerve/bladder revealed schwann cell death, axon degeneration and collagen remodeling of bladder. Polymerase Chain Reaction results revealed vigorous nerve inflammation and bladder fibrosis 1 week after injury and inflammation/fibrosis returned to normal at 4 w. …”
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Ox-LDL induced profound changes of small non-coding RNA in rat endothelial cells by Yu Wang, Tianhua Liu, Wenying Xiao, Yanyan Bai, Dandan Yue, Liuliu Feng

“…The expression changes of sncRNA were analyzed by small RNA high-throughput sequencing, and the expression changes of piRNA, snoRNA, snRNA, miRNA and repeat-associated RNA were verified by quantitative polymerase chain reaction (qPCR).ResultsSmall RNA sequencing showed that 42 piRNAs were upregulated and 38 piRNAs were downregulated in endothelial cells treated with ox-LDL. …”
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Activation of CREB-binding protein ameliorates spinal cord injury in tabersonine treatment by suppressing NLRP3/Notch signaling by Yu Ming Chuan, Yu Wang, Xu Jin, Shi Qing Ming, Wan Wen Bing, Wu Kai, Chen Xiang, Peng Kun

“…Moreover, mediators of inflammation were estimated using enzyme-linked immunosorbent assay (ELISA) and the effect of tabersonine on Notch/inflammasome signaling was estimated by reverse transcription polymerase chain reaction (RT-PCR), western blot assay and immunohistochemistry. …”
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Highly sensitive and rapid identification of coxsackievirus A16 based on reverse transcription multiple cross displacement amplification combined with nanoparticle-based lateral fl... by Jinzhi Cheng, Yu Wang, Yuhong Zhou, Jingrun Lu, Xiaomin Tang

“…The findings demonstrated that the CVA16-MCDA test could promptly and successfully identify all of the CVA16-positive (46/220) samples identified by the traditional real-time quantitative polymerase chain reaction (qRT-PCR) assays for 220 clinical anal swab samples. …”
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CircCDK17 knockdown inhibits tumor progression and cell glycolysis by downregulaing YWHAZ expression through sponging miR-1294 in cervical cancer by Rui Chen, Fei Liang, Jun Yan, Yu Wang

“…Methods The expression levels of circCDK17, microRNA-1294 (miR-1294) and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) mRNA were detected by quantitative real time polymerase chain reaction (qRT-PCR). The protein expression levels of YWHAZ, recombinant glucose transporter 1 (GLUT1) and hexokinase 2 (HK2) were determined by western blot. …”
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Identification of reliable reference genes for gene expression studies in mouse models under microplastics stress by Ju Mu, Yu Wang, Miao Wang, Dayi Zhang, Mingying Liu

“…Reverse transcriptase quantitative real-time polymerase chain reaction (RT-qPCR) serves as a powerful tool to assess gene expression, however highly dependent on a reliable reference gene. …”
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The Bcl-2-associated athanogene gene family in tobacco (Nicotiana tabacum) and the function of NtBAG5 in leaf senescence by Linxin Gu, Bing Hou, Xiao Chen, Yu Wang, Pingan Chang, Xiaohong He, Daping Gong, Quan Sun

“…RNA-seq and real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) revealed that the expression of NtBAG5c–f and NtBAG6a–b was upregulated in senescent leaves, implying that they play a role in regulating leaf senescence. …”
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Analysis of the key ligand receptor CADM1_CADM1 in the regulation of thyroid cancer based on scRNA-seq and bulk RNA-seq data by Hui He, Hui He, Hui He, Shan Cong, Yu Wang, Yu Wang, Qinghai Ji, Qinghai Ji, Weiyan Liu, Ning Qu, Ning Qu

“…Moreover, the expression levels of CADM1-CADM1 was assessed by quantitative real time polymerase chain reaction (qRT-PCR) and the function was analyzed using Transwell immigration assay.ResultsWe found that CADM1_CADM1 could be regarded as a biomarker representing a good prognosis of PTC. …”
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Novel hub genes and regulatory network related to ferroptosis in tetralogy of Fallot by Yu Wang, Yu Wang, Junjie Yang, Junjie Yang, Jieru Lu, Jieru Lu, Qingjie Wang, Jian Wang, Jianyuan Zhao, Yuqiang Huang, Kun Sun, Kun Sun

“…The expression of hub genes was further validated through quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR). After conducting the differential gene analysis, we observed that in TOF, 41 upregulated mRNAs and three downregulated mRNAs associated with ferroptosis genes were found. …”
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Denitrification contributes to N2O emission in paddy soils by Hua Xiang, Hua Xiang, Hua Xiang, Yiguo Hong, Jiapeng Wu, Yu Wang, Fei Ye, Jiaqi Ye, Jing Lu, Aimin Long, Aimin Long

“…In this study, the potential N2O emission rate, enzymatic activity for N2O production and reduction, gene abundance, and community composition during denitrification were investigated using the 15N isotope tracer technique combined with slurry incubation, enzymatic activity detection, quantitative polymerase chain reaction (qPCR), and metagenomic sequencing. …”
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Verification of cell cycle-associated cyclin-dependent kinases facilitated prostate cancer progression by integrated bioinformatic analysis and experimental validation by Yean Huang, Shuo Lu, Yi Chen, Yunhao Qing, Ruji Wu, Tan Ma, Zixiao Zhang, Yu Wang, Ke Li

“…The expression of CDKs in tissue was examined through quantitative real-time polymerase chain reaction. After that, we focused on CDK3 and identified the expression of CDK3 by immunohistochemistry and western blot. …”
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A novel loop-mediated isothermal amplification-lateral flow dipstick method for Helicobacter pylori detection by Wenwen Liu, Gang Lu, Gang Lu, Yu Wang, Yu Wang, Zhenghong Chen, Yunyun Gao, Zhipeng Yin, Yi Wu, Xiaoqian Lv, Pengbo Guo, Pengbo Guo, Yinghui Zhao, Yinghui Zhao

“…The lower limit of the detection method was 102 copies/μL, which was 100 times the sensitivity of polymerase chain reaction (PCR). H. pylori-positive fecal samples were detected by LAMP-LFD in 13/20 patients.DiscussionIn conclusion, a new LAMP-LFD assay has been fully established and confirmed for H. pylori detection. …”
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Integrated analysis of tRNA-derived small RNAs in proliferative human aortic smooth muscle cells by Jian-Zhi Zhao, Qi-Yao Li, Jia-Jie Lin, Li-Yun Yang, Mei-Yang Du, Yu Wang, Ke-Xin Liu, Ze-An Jiang, Huan-Huan Li, Si-Fan Wang, Bo Sun, Shi-Qing Mu, Bin Li, Kun Liu, Miao Gong, Shao-Guang Sun

“…Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the sequence results and subcellular distribution of AS-tDR-001370, AS-tDR-000067, AS-tDR-009512, and AS-tDR-000076. …”
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Epidemiological Survey and Risk Factor Analysis of 14 Potential Pathogens in Golden Snub-Nosed Monkeys at Shennongjia National Nature Reserve, China by Mingpu Qi, Qiankun Wang, Yu Wang, Yingyu Chen, Changmin Hu, Wanji Yang, Feng Wu, Tianpeng Huang, Ali Sobhy Dawood, Muhammad Zubair, Xiang Li, Jianguo Chen, Ian Duncan Robertson, Huanchun Chen, Aizhen Guo

“…In addition, fecal Adenovirus and Rotavirus were detected using Polymerase Chain Reaction (PCR). As a result, the Macacine herpesvirus-1 (MaHV-1), Golden snub-nosed monkey cytomegalovirus (GsmCMV), Simian foamy virus (SFV) and Hepatitis A virus (HAV) were detected with the seroprevalence of 57.7% (95% CI: 36.9, 76.6), 38.5% (95% CI: 20.2, 59.4), 26.9% (95% CI: 11.6, 47.8), and 7.7% (95% CI: 0.0, 84.2), respectively. …”
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Preemptive interferon-α therapy could prevent relapse of acute myeloid leukemia following allogeneic hematopoietic stem cell transplantation: A real-world analysis by Shuang Fan, Tian-Zhong Pan, Li-Ping Dou, Yan-Min Zhao, Xiao-Hui Zhang, Lan-Ping Xu, Yu Wang, Xiao-Jun Huang, Xiao-Jun Huang, Xiao-Jun Huang, Xiao-Dong Mo, Xiao-Dong Mo

“…The protocols for MRD monitoring mainly based on quantitative polymerase chain reaction [qPCR] and multiparameter flow cytometry [MFC]. …”
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