Use of megaprimer and overlapping extension pcr (OE-PCR) to mutagenize and enhance cyclodextrin glucosyltransferase (CGTASE) function by Goh, Kian Mau, Liew, Kok Jun, Chai, Kian Piaw, Md. Illias, Rosli

“…In this chapter, we describe two protocols for performing site-directed mutagenesis of any protein-coding sequence, namely, megaprimer PCR and overlapping extension PCR (OE-PCR). …”

Mutation in plant: key of successful agriculture industry by Mohamed, Elizah, Adham, Nur Elmira Shafiqah, Mohd Esa, Nur Adilah Fitri, Abd Aziz, Mohd Haiqal

“…The development in mutation breeding is highly reliant on multiple biotechnologies, including mutagenesis methods, dissociation of the chimera, enhancement of homozygous mutants in seed harvests, and improvements in successful screening and freak assessment. …”
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