The analysis of DNA interstrand cross-links and their repair by the modified comet assay by Castle, L, Swift, LP, McHugh, P

“…The sensitive detection of ICLs in mammalian cells is challenging but has been aided by the development of a modified form of the single-cell gel electrophoresis (SCGE) assay, also known as the “comet assay.” Here we describe this method and how it can be used to sensitively monitor the induction and removal of ICLs in single mammalian cells.…”