Protein cage assisted metal-protein nanocomposite synthesis : optimization of loading conditions by Sana, Barindra, Calista, Marcia, Lim, Sierin

“…The metallic core formed within the Archaeoglobus fulgidus ferritin cavity is stabilized by modulating the protein structure by site directed mutagenesis. Encapsulation protocol of various metals within the engineered ferritin cage (AfFtn-AA) is optimized. …”
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The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II by Jeon, Choon Ju, Yoon, Ho Sup, Agarwal, Kan

“…The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant residues Asp-261 and Glu-262 of the nucleic acid-binding TFIIS Zn ribbon are critical for stimulation of both elongation and RNA cleavage activities of RNA polymerase II. …”
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Investigating the influence of ionic concentrations and subunit interactions on the self-assembly of E2 protein by Peng, Tao, Tan, Sze Wah, Dharmawan, Ratna Ekawati, Lim, Sierin

“…To assess intrinsic factors that influence E2 self-assembly, we identified some key amino acids at interfaces of subunits and performed site-directed mutagenesis on them. Characterizations of each mutant for size and secondary structure contents were performed. …”
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Functional characterisation of SV2C and its variants as a Parkinson’s Disease-associated gene by Chang, Chu-Hua, Chew, Elaine Guo Yan, Tandiono, Moses, Zhang, Tian, Gong, Ximing, Xia, Yun, Foo, Jia Nee

“…CRISPR/Cas9-mediated site-specific mutagenesis will also be performed to generate SV2C D543N variant in H9 cells and the N-glycosylation of SV2C will be investigated. …”
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