THE POLYMERASE CHAIN-REACTION IN PATHOLOGY by Pezzella, F, Mason, D

Increases in DNA lesions and the DNA damage indicator Gadd45 following transient cerebral ischemia. by Hou, S, Tu, Y, Buchan, A, Huang, Z, Preston, E, Rasquinha, I, Robertson, G, MacManus, J

“…By 6 h, gadd45 mRNA was shown to increase by semi-quantitative reverse transcriptase - polymerase chain reaction. In situ hybridization histochemistry indicated that these increases in gadd45 mRNA occurred in pyramidal neurons located on the edge of the infarcted cortex. …”

Chemokine receptor polymorphisms and human immunodeficiency virus disease progression. by Easterbrook, P, Rostron, T, Ives, N, Troop, M, Gazzard, BG, Rowland-Jones, S

“…Genotyping was done by use of amplification refractory mutation system-polymerase chain reaction with sequence-specific primers, and Cox proportional hazards models were used to examine the impact of polymorphisms on time to a CD4 cell count <200x106/L and to CDC stage IV disease. …”

Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency. by MacDonald, S, Bamforth, S, Chen, C, Farthing, C, Franklyn, A, Broadbent, C, Schneider, J, Saga, Y, Lewandoski, M, Bhattacharya, S

“…Embryos were examined by magnetic resonance imaging, by sectioning and by quantitative real-time polymerase chain reaction (qRT-PCR). Epiblast-specific deletion of Cited2 using Sox2Cre recapitulated penetrant and phenotypically heterogenous cardiovascular and laterality defects. …”