Site directed mutagenesis of alkyl hydroperoxide reductase. by Tee, Chiu Seng.

“…This research was designed to investigate the binding site of nicotinamide adenine dinucleotide (NADH) on alkyl hydroperoxide reductase subunit F (AhpF) of Salmonella typhimurium by using site directed mutagenesis. The postulated binding site was suggested by using molecular mechanics Poisson–Boltzmann surface area (MM/PBSA) approach. …”
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Alteration of coenzyme specificity of alkyl hydroperoxide reductase by site directed mutagenesis by B K Nirmalee Lakshani Goonewardena

“…The gene coding for AhpF product has been successfully modified using site directed mutagenesis and cloned into two vectors pJET and pET30b for long term storage and protein expression respectively. …”
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Change hydrogen-peroxide forming NADH oxidase substrate specificity by site directed mutagenesis by Choong, Carmen.

“…Site directed mutagenesis is a molecular biology technique used to determine the biological roles of many proteins. …”
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Structural and mutagenesis studies of the NS3 helicase from dengue virus for drug discovery by Yang, Xiaoqing

“…With reference to the structure analysis of RNA unwinding mechanism coupled with ATP hydrolysis by Dengue virus (DEN) non-structural 3 (NS3) protein (Luo et al., 2008a), site-directed mutagenesis on DEN NS3 helicase domain was performed. …”
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Insertional mutagenesis identifies Dph3 as a novel regulator of melanoma migration and invasion. by Yeo, Siok Ping.

“…Thus, there is a need to look for new treatment options. Using insertional mutagenesis, we identified Rnf11, Dph3, Zmynd10 and Prdm5 as potential regulators of melanoma metastasis. …”
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Site-directed mutagenesis of E2 protein nanocage for the tunable display of siRNA by Zennathul Firdous Mohamed Barak

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Mutagenesis and characterization studies of E. Coli DNA-binding protein from starved cells (DPS) by Ang, Emmiline Xiao Wei

“…Ferritins are proteins that form spherical protein cages surrounding nanacavities filled with iron.E.coli DNA-binding protein from starved cell (DPS), a mini-ferritin capable of self-assembling into 12-meric (twelve monomers) nanocages, was chosen as the basis for an alanine-shaving mutagenesis study to investigate the importance of key amino acid in controlling protein stability and self-assembly.…”
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Characterisation of sox6 function in zebrafish using mutant alleles generated by Zinc Finger Nuclease (ZFN)-mediated targeted mutagenesis. by Kong, Hui Yi.

“…Mutant alleles of the sox6 gene in zebrafish Danio rerio have been generated using Zinc finger nuclease (ZFN) targeted mutagenesis. In situ hybridization results revealed a requirement for Sox6, together with Sox5, in oligodendrocyte specification. …”
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Site-directed mutagenesis of p97/VCP to gain insight on the role of ATP binding to D1 domain affecting adaptor protein selectivity. by Rizal Ismail.

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Generation of Pseudomonas mutants for investigating a model mixed species biofilm by Neo, Desmond

“…Deletion mutagenesis of differentially expressed genes in Pf-5 were performed, with PFL_5251 as the target gene to develop a reliable method for deletion mutagenesis in Pf-5 where multiple key methods were identified at each stage. …”
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Laboratory evolution of microalgae species, chlorella sorokiniana, for usage in sustainable food production by Lim, Ghim Leung

“…Although there is increased interest in improving microalgae strains for usage in food applications, there are a lack of current research available on the synergistic effects of random mutagenesis and adaptive laboratory evolution (ALE) on microalgae. …”
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Laboratory evolution of microalgae for food applications by Ho, Ivan Yan De

“…The research performs UV and EMS mutagenesis of chlorella sorokiniana and cultivating them in salinity stress. …”
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Identifying genes involved in neuronal differentiation of oln-93 oligodendroglia cells. by Huang, Yi Xia.

“…In this study, we used insertional mutagenesis to identify TSP-4, NZF-1, and Pgk-1 as potential regulators of neuronal differentiation. …”
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Dph3 is involved in migration and proliferation of melanoma cells. by Sin, Yen Suan.

“…Preliminary study has been done to identify Dph3 gene as potential factor of melanoma metastasis by using insertional mutagenesis. Here, we show that the expression of Dph3 gene is associated with melanoma cell migration. …”
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Analysis of molecular mechanisms of cardiac arrhythmia. by Wong, Samantha Zhu Er.

“…Polymorphic variants of KCNH2 were also generated via site-directed mutagenesis to raise stable zebrafish transgenic lines. 25% of the progeny of these heterozygotic lines would express KCNH2-variant mutated hearts and these embryos would be used to analyze zebrafish cardiac electrical profile.…”
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RING Finger Protein 11 promotes melanoma metastasis via TGF- β signaling. by Xu, Ruiping.

“…Preliminary study has identified RNF11 as a potential regulator of melanoma metastasis by using insertional mutagenesis. RING finger protein 11 is a 154-amino acid modular protein that is expressed ubiquitously in human. …”
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Analysis of zebrafish mutants from Ac/Ds transposon screen. by Koh, Eirene Rae Jia Xuan.

“…These traits allow zebrafish to be easily studied using mutagenesis. The aim of this project is to screen out homozygous zebrafish mutants generated by Ac/Ds transposon system. …”
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Molecular mechanism of plant cellulose biosynthesis through structural investigation by Kong, Sean Yao Zu

“…The second goal is to crystallise the protein by performing crystal screening and surface residue mutations. Site-directed mutagenesis was performed on the catalytic domain of wild-type Arabidopsis thaliana (At) cesa8 gene to facilitate protein-protein crystal contacts. …”
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Characterization of a novel JNK2-interacting protein, C331. by Goh, Weibin.

“…Using site-directed mutagenesis, the D-domain was disrupted in C331 by sequential replacement of basic residues with alanine. …”
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Identification of microRNA targets for Isoforms of pro-apoptotic factor BIM. by Hong, Hui Qi.

“…Subsequently, we introduced serial deletions in these 3’UTRs using polymerase chain reaction (PCR) mutagenesis. Comparing reporter gene expression level of constructs containing any of the wild-type BIM 3’UTRs with their mutant counterparts afforded identification of functional cis-regulatory clusters. …”
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