WIP promotes in-vitro invasion ability, anchorage independent growth and EMT progression of A549 lung adenocarcinoma cells by regulating RhoA levels by Salvi, Amrita, Thanabalu, Thirumaran

“…Knocking down WIP expression in A549 cells significantly reduced RhoA levels and WIP was found to interact with RhoA suggesting that WIP might be executing its function by regulating RhoA. …”
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Mitigating Diseases Transmitted by Aedes Mosquitoes: A Cluster-Randomised Trial of Permethrin-Impregnated School Uniforms by Kittayapong, Pattamaporn, Olanratmanee, Phanthip, Maskhao, Pongsri, Byass, Peter, Logan, James, Tozan, Yesim, Louis, Valérie, Gubler, Duane J., Wilder-Smith, Annelies

“…WHOPES cone tests showed a 100% knock down and mortality of Aedes aegypti mosquitoes exposed to impregnated clothing at baseline and up to 4 washes, but this efficacy rapidly declined to below 20% after 20 washes, corresponding to a weekly reduction in knock-down and mosquito mortality by 4.7% and 4.4% respectively. …”
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Parkin deficiency exacerbates fasting-induced skeletal muscle wasting in mice by Peker, Nesibe, Sharma, Mridula, Kambadur, Ravi

“…Here, results suggested that knock down of Parkin significantly increases proteolytic activities in skeletal muscle cell line, the C2C12 myotubes. …”
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Layer-by-layer nanoparticles as an efficient siRNA delivery vehicle for SPARC silencing by Tan, Yang Fei, Chen, Min Hui Averil, Lessig, Jacqueline, Neu, Björn, Mundargi, Raghavendra C., Venkatraman, Subbu S., Wong, Tina T.

“…Significant (60%) SPARC-gene knock down was achieved by using 0.4 pmole siRNA/μg of LbL NPs in FibroGRO cells and the relative expression of SPARC mRNA reduced significantly (60%) against untreated cells. …”
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The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex by Tie, Hieng Chiong, Madugula, Viswanadh, Lu, Lei

“…When endogenous nucleoporins were knocked down and replaced by the exogenously expressed and knockdown-resistant GFP-nucleoporins, the stoichiometry of the nucleoporin was estimated by the ratio of its fluorescence intensity to that of the GFP single molecules. …”
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Thermo-mechanical debonding map for repairing composite–metal adhesive joints by Srinivasan, Dharun Vadugappatty, Idapalapati, Sridhar

“…The debonding region was identified in the temperature range between 1.17 Tg and 1.33 Tg. The knock-down in storage modulus and initial peel load exhibited a similar trend with respect to the temperature.…”
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p53-independent regulation of p21Waf1/Cip1 expression and senescence by PRMT6 by Phalke, Sameer, Mzoughi, Slim, Bezzi, Marco, Jennifer, Nancy, Mok, Wei Chuen, Thike, Aye Aye, Tan, Puay Hoon, Voorhoeve, P. Mathijs, Guccione, Ernesto, Low, Diana H. P., Kuznetsov, Vladimir A.

“…Here, we report that protein arginine methyltransferase 6 (PRMT6), a type I PRMT known to act as a transcriptional cofactor, directly represses the p21 promoter. PRMT6 knock-down (KD) results in a p21 derepression in breast cancer cells, which is p53-independent, and leads to cell cycle arrest, cellular senescence and reduced growth in soft agar assays and in severe combined immunodeficiency (SCID) mice for all the cancer lines examined. …”
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Role of NMDA receptors in adult neurogenesis and normal development of the dentate gyrus by Åmellem, Ingrid, Yovianto, Glen, Chong, Hai Tarng, Nair, Rajeevkumar Raveendran, Cnops, Vanja, Thanawalla, Ayesha, Tashiro, Ayumu

“…In this study, we investigate this question by examining a region-specific knock-out mouse line that lacks the NR1 gene, which encodes the essential subunit of the NMDA receptors, in granule cells of the dentate gyrus (DG-NR1KO mice). …”
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SRRM2 organizes splicing condensates to regulate alternative splicing by Xu, Shaohai, Lai, Soak-Kuan, Sim, Donald Yuhui, Ang, Warren Shou Leong, Li, Hoi Yeung, Roca, Xavier

“…Using mainly EGFP-SRRM2 knock-in HEK293T cells, we show that SRRM2 forms biomolecular condensates satisfying most hallmarks of liquid-liquid phase separation, including spherical shape, dynamic rearrangement, coalescence and concentration dependence supported by in vitro experiments. …”
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Enhancing damping-like efficiency by low-energy mixed ions bombardment by Bhatti, Sabpreet, Das, Subhakant, Shaik, Abdillah, Chan, Jianpeng, Kay, Yew Seng, Li, Shengyao, Piramanayagam, S. N.

“…The bombardment modifies the physical properties (magnetic, electric, and structural) due to cascade collision, knocking off atoms (from the surface), and ion implantation. …”
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POPX2 is a novel LATS phosphatase that regulates the Hippo pathway by Muhammad Bakhait Rahmat, Zhang, Songjing, Koh, Cheng-Gee

“…As a result, YAP/TAZ are not phosphorylated and are able to translocate into the nucleus to activate target genes involved in cell proliferation. Furthermore, POPX2 knock-out using CRISPR in the highly metastatic MDA-MB-231 breast cancer cells results in decreased cell proliferation and impairment of anchorage independent growth. …”
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Targeting Cx26 expression by sustained release of Cx26 antisense from scaffolds reduces inflammation and improves wound healing by Phillips, Anthony R.J., Chin, Jiah Shin, Madden, Leigh, Gilmartin, Daniel J., Soon, Allyson, Thrasivoulou, Christopher, Jayasinghe, Suwan N., Miles, Michelle, O'Neill, Shay, Hu, Rebecca, Chew, Sing Yian, Becker, David Lawrence

“…If the same scaffolds are coated with an antisense molecule specifically targeting Cx26 that has a slow sustained release, this prevents the abnormal upregulation of Cx26 protein at the wound edge. Knocking down Cx26 protein levels below those seen in normal wound healing has no adverse effects on the healing process but instead reduces the epidermal thickening and also the inflammatory response, while at the same time promotes the healing response. …”
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An essential role for Grk2 in Hedgehog signalling downstream of Smoothened by Zhao, Zhonghua, Lee, Raymond Teck Ho, Pusapati, Ganesh V., Iyu, Audrey, Rohatgi, Rajat, Ingham, Philip William

“…The G-protein-coupled receptor kinase 2 (adrbk2/GRK2) has been implicated in vertebrate Hedgehog (Hh) signalling based on the effects of its transient knock-down in mammalian cells and zebrafish embryos. …”
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A human expression system based on HEK293 for the stable production of recombinant erythropoietin by Chin, Christine Lin, Goh, Justin Bryan, Srinivasan, Harini, Liu, Kaiwen Ivy, Gowher, Ali, Shanmugam, Raghuvaran, Lim, Hsueh Lee, Choo, Matthew, Tang, Wen Qin, Tan, Andy Hee-Meng, Nguyen-Khuong, Terry, Tan, Meng How, Ng, Say Kong

“…Hence, we developed a fully human expression system based on HEK293 cells for the stable and high titer production of recombinant proteins by first knocking out GLUL (encoding glutamine synthetase) using CRISPR-Cas9 system. …”
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Zyxin is involved in fibroblast rigidity sensing and durotaxis by Yip, Ai Kia, Zhang, Songjing, Chong, Lor Huai, Cheruba, Elsie, Woon, Jessie Yong Xing, Chua, Theng Xuan, Goh, Corinna Jie Hui, Yang, Haibo, Tay, Chor Yong, Koh, Cheng-Gee, Chiam, Keng-Hwee

“…Zyxin is a component of the FA that has been implicated in connecting the actin cytoskeleton to the FA. We have found that knocking down zyxin impaired NIH3T3 fibroblast's ability to sense and respond to changes in extracellular matrix in terms of their FA sizes, cell traction stress magnitudes and F-actin organization. …”
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Impaired episodic-like memory in a mouse model of Alzheimer's disease is associated with hyperactivity in prefrontal-hippocampal regions by Tan, Sijie, Tong, Wen Han, Vyas, Ajai

“…Alzheimer's disease (AD) is a degenerative brain disorder with a long prodromal period. An APPNL-G-F knock-in mouse model is a preclinical model to study incipient pathologies during the early stages of AD. …”
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VarR controls colonization and virulence in the marine macroalgal pathogen Nautella italica R11 by Gardiner, Melissa, Fernandes, Neil D., Nowakowski, Dennis, Raftery, Mark, Kjelleberg, Staffan, Zhong, Ling, Thomas, Torsten, Egan, Suhelen

“…We show here that a varR knock-out strain is deficient in its ability to cause disease in D. pulchra and is defective in biofilm formation and attachment to a common algal polysaccharide. …”
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Blocking sense‐strand activity improves potency, safety and specificity of anti‐hepatitis B virus short hairpin RNA by Michler, Thomas, Große, Stefanie, Mockenhaupt, Stefan, Röder, Natalie, Stückler, Ferdinand, Knapp, Bettina, Ko, Chunkyu, Heikenwalder, Mathias, Protzer, Ulrike, Grimm, Dirk

“…Importantly, the novel AAV8 vector co‐expressing anti‐HBV shRNA and TuD outperformed all other strategies regarding efficiency and persistence of HBV knock‐down, thus showing substantial promise for clinical translation.…”
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Viable mice with extensive gene humanization (25-kbp) created using embryonic stem cell/blastocyst and CRISPR/zygote injection approaches by Leidy-Davis, Tiffany, Cheng, Kai, Goodwin, Leslie O., Morgan, Judith L., Juan, Wen Chun, Roca, Xavier, Ong, S. Tiong, Bergstrom, David E.

“…Here, we describe an expansion of the typical DNA size limitations associated with CRISPR knock-in technology, more specifically, the physical extent to which mouse genomic DNA can be replaced with donor (in this case, human) DNA at an orthologous locus by zygotic injection. …”
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Pomc plays an important role in sexual size dimorphism in tilapia by Wan, Zi Yi, Lin, Valerie Chun Ling, Hua, Yue Gen

“…In vitro luciferase assay of the two elements revealed that β-estradiol significantly enhanced the expression of luciferase activity, suggesting that the expression of Pomc is mediated by estrogen. We knocked out Pomc in zebrafish using Crispr/Cas-9. …”
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