Viable mice with extensive gene humanization (25-kbp) created using embryonic stem cell/blastocyst and CRISPR/zygote injection approaches by Leidy-Davis, Tiffany, Cheng, Kai, Goodwin, Leslie O., Morgan, Judith L., Juan, Wen Chun, Roca, Xavier, Ong, S. Tiong, Bergstrom, David E.

“…Using a unique second method, we employed the same vector (devoid of its selectable marker cassettes), microinjecting it along with redundant single guide RNAs (sgRNAs) and Cas9 mRNA into mouse zygotes (CRISPR/Zygote Approach). In both instances, we were able to achieve humanization of Bcl2l11 to the extent designed, remove all selection cassettes, and demonstrate the functionality of the conditionally removable, loxP-flanked, 2.9-kbp intronic segment.…”
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Selective binding of retrotransposons by ZFP352 facilitates the timely dissolution of totipotency network by Li, Zhengyi, Xu, Haiyan, Li, Jiaqun, Xu, Xiao, Wang, Junjiao, Wu, Danya, Zhang, Jiateng, Liu, Juan, Xue, Ziwei, Zhan, Guankai, Tan, Bobby Cheng Peow, Chen, Di, Chan, Yun-Shen, Ng, Huck Hui, Liu, Wanlu, Hsu, Chih-Hung, Zhang, Dan, Shen, Yang, Liang, Hongqing

“…Currently, extensive insights have been revealed for the totipotency regulatory network around the zygotic genome activation (ZGA) period. However, how the dissolution of the totipotency network is triggered to ensure the timely embryonic development following ZGA is largely unknown. …”
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