Decreased GLUT4 translocation underlies the cardiomyopathy associated with Duchenne muscular dystrophy by Sang, A, Bia, B, Wilding, JR, Radda, G, Davies, K, Clarke, K

Lower maternal body condition during pregnancy affects skeletal muscle structure and glut-4 protein levels but not glucose tolerance in mature adult sheep. by Costello, P, Hollis, L, Cripps, R, Bearpark, N, Patel, H, Sayer, A, Cooper, C, Hanson, M, Ozanne, SE, Green, L

Differential translocation of the fatty acid transporter, FAT/CD36, and the glucose transporter, GLUT4, coordinates changes in cardiac substrate metabolism during ischemia and reperfusion. by Heather, L, Pates, K, Atherton, H, Cole, M, Ball, DR, Evans, R, Glatz, J, Luiken, J, Griffin, J, Clarke, K

“…CONCLUSIONS: During ischemia, FAT/CD36 moved away from the sarcolemma as GLUT4 moved toward the sarcolemma, associated with a shift from fatty acid oxidation to glycolysis, while intramyocardial lipid accumulation was prevented. …”

PPAR-alpha activation required for decreased glucose uptake and increased susceptibility to injury during ischemia. by Panagia, M, Gibbons, G, Radda, G, Clarke, K

“…We conclude that elevated plasma free fatty acids are associated with increased injury during ischemia due to decreased cardiac glucose uptake resulting from lower cardiac GLUT-4 protein levels, the levels of GLUT-4 being regulated, probably indirectly, through PPAR-alpha activation.…”

Insulin resistance, abnormal energy metabolism and increased ischemic damage in the chronically infarcted rat heart. by Murray, A, Lygate, C, Cole, M, Carr, C, Radda, G, Neubauer, S, Clarke, K

“…Here, we tested whether there is a link between insulin resistance and ischemic damage in the chronically infarcted Wistar rat heart, postulating that the heart would have decreased insulin sensitivity, with lower GLUT4 glucose transporter protein levels due to high circulating free fatty acid (FFA) concentrations. …”

Uncoupling proteins in human heart. by Murray, A, Anderson, R, Watson, G, Radda, G, Clarke, K

“…When plasma free-fatty-acid concentrations were raised, cardiac mitochondrial uncoupling proteins (UCP) increased (isoform UCP2, p<0.0001; isoform UCP3, p=0.0036) and those of glucose transporter (GLUT4) protein decreased (cardiac, p=0.0001; skeletal muscle, p=0.0006). …”

Changes in cardiac substrate transporters and metabolic proteins mirror the metabolic shift in patients with aortic stenosis. by Heather, L, Howell, N, Emmanuel, Y, Cole, M, Frenneaux, M, Pagano, D, Clarke, K

“…FAT/CD36 and complex I of the electron transport chain were downregulated, whereas the glucose transporter GLUT4 was upregulated with increasing left ventricular mass index, a measure of cardiac hypertrophy. …”

Thiazolidinedione treatment normalizes insulin resistance and ischemic injury in the zucker Fatty rat heart. by Sidell, R, Cole, M, Draper, N, Desrois, M, Buckingham, R, Clarke, K

“…At 12 months of age, obese rat hearts were insulin resistant with decreased GLUT4 protein expression. During ischemia, glucose uptake was lower and depletion of ATP was greater in obese rat hearts, thereby significantly impairing recovery of contractile function during reperfusion. …”

Proteomic analysis of human adipose tissue after rosiglitazone treatment shows coordinated changes to promote glucose uptake. by Ahmed, M, Neville, M, Edelmann, M, Kessler, B, Karpe, F

“…Thus, the major changes observed were among proteins involved in cytoskeletal rearrangement, insulin and calcium signaling, and inflammatory and redox signals that decisively upregulate GLUT4 granule trafficking in human adipose tissue. …”

Adenosine monophosphate-activated protein kinase activation, substrate transporter translocation, and metabolism in the contracting hyperthyroid rat heart. by Heather, L, Cole, M, Atherton, H, Coumans, W, Evans, R, Tyler, D, Glatz, J, Luiken, J, Clarke, K

“…The combined effects of T(3)-induced AMPK activation and insulin stimulation were associated with increased sarcolemmal GLUT4 localization and glycolytic flux in the hyperthyroid heart.…”

Human hippocampal energy metabolism is impaired during cognitive activity in a lipid infusion model of insulin resistance. by Emmanuel, Y, Cochlin, L, Tyler, D, de Jager, C, Smith, A, Clarke, K

“…However, it is now known that components of the insulin-mediated glucose uptake pathway, including neuronal insulin synthesis and the insulin-dependent glucose transporter GLUT4, are present in brain tissue, particularly in the hippocampus. …”

Initial steps of insulin signaling and glucose transport are defective in the type 2 diabetic rat heart. by Desrois, M, Sidell, R, Gauguier, D, King, L, Radda, G, Clarke, K

“…CONCLUSION: We conclude that decreased insulin receptor beta, IRS-1 and GLUT4 proteins are associated with insulin resistance in type 2 diabetic rat hearts.…”

Peri-implantation and late gestation maternal undernutrition differentially affect fetal sheep skeletal muscle development. by Costello, P, Rowlerson, A, Astaman, N, Anthony, F, Sayer, A, Cooper, C, Hanson, M, Green, L

“…The predominant effect in the L group was on slow-twitch myofibres. In TB, InsR, GLUT-4 and IGF-1R mRNA levels were greater in L group fetuses. …”

Human hippocampal energy metabolism is impaired during cognitive activity in a lipid infusion model of insulin resistance by Emmanuel, Y, Cochlin, L, Tyler, D, De Jager, C, Smith, A, Clarke, K

“…However, it is now known that components of the insulin‐mediated glucose uptake pathway, including neuronal insulin synthesis and the insulin‐dependent glucose transporter GLUT4, are present in brain tissue, particularly in the hippocampus. …”

Mammalian glucose transporter activity is dependent upon anionic and conical phospholipids by Hresko, R, Kraft, T, Quigley, A, Carpenter, E, Hruz, P

“…We systematically examined the effects of physiologically relevant phospholipids on glucose transport in liposomes containing purified GLUT4 and GLUT3. The anionic phospholipids, phosphatidic acid, phosphatidylserine, phosphatidylglycerol, and phosphatidylinositol, were found to be essential for transporter function by activating it and stabilizing its structure. …”

Abnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart. by Panagia, M, Schneider, J, Brown, B, Cole, M, Clarke, K

“…Glucose uptake during ischemia was 21% lower than in controls, and post-ischemic recovery of cardiac function was decreased by 30% in db/db mouse hearts (p<0.05). Total cardiac GLUT 4 protein was 56% lower (p<0.01) in db/db mice than in controls. …”

Isoproterenol induces in vivo functional and metabolic abnormalities: similar to those found in the infarcted rat heart. by Heather, L, Catchpole, A, Stuckey, D, Cole, M, Carr, C, Clarke, K

“…Basal glycolysis and glycogen concentration were not changed, yet insulin stimulated glycolysis was decreased by 32%, accompanied by 33% lower insulin stimulated glucose transporter, GLUT4, protein levels in rat hearts following isoproterenol infusion, compared with controls. …”

Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level. by Garcia-Rua, V, Feijoo-Bandin, S, Garcia-Vence, M, Aragon-Herrera, A, Bravo, S, Rodriguez-Penas, D, Mosquera-Leal, A, Lear, P, Parrington, J, Alonso, J, Rosello-Lleti, E, Portoles, M, Rivera, M, Gonzalez-Juanatey, J, Lago, F

“…Finally, in vitro experiments performed in neonatal rat cardiomyocytes, in which TPCN1 was silenced using siRNAs, confirmed that the downregulation of TPCN1 gene expression increased 2-deoxy-D-[3H]-glucose uptake and GLUT4 mobilization into cell peripherals in cardiac cells. …”

Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level by García-Rúa, V, Feijóo-Bandín, S, García-Vence, M, Aragón-Herrera, A, Bravo, S, Rodríguez-Penas, D, Mosquera-Leal, A, Lear, P, Parrington, J, Alonso, J, Roselló-Lletí, E, Portolés, M, Rivera, M, González-Juanatey, J, Lago, F

“…Finally, in vitro experiments performed in neonatal rat cardiomyocytes, in which TPCN1 was silenced using siRNAs, confirmed that the downregulation of TPCN1 gene expression increased 2-deoxy-D-[3H]-glucose uptake and GLUT4 mobilization into cell peripherals in cardiac cells. …”

Gender differences in hypertrophy, insulin resistance and ischemic injury in the aging type 2 diabetic rat heart. by Desrois, M, Sidell, R, Gauguier, D, Davey, C, Radda, G, Clarke, K

“…Glucose transporter (GLUT) 1 protein levels were the same in all hearts, but GLUT4 protein levels were 28% lower (P < 0.01) in all GK rat hearts compared with their sex-matched controls. …”