Radiochemical HPLC detection of arginine metabolism: measurement of nitric oxide synthesis and arginase activity in vascular tissue. by de Bono, J, Warrick, N, Bendall, J, Channon, K, Alp, N

“…Accurate measurement of NO production by endothelial nitric oxide synthase (eNOS) is critical for the investigation of vascular disease mechanisms using genetically modified animal models. Previous assays of NO production measuring the conversion of arginine to citrulline have required homogenisation of tissue and reconstitution with cofactors including NADPH and tetrahydrobiopterin. …”

Housing, husbandry and handling of rodents for behavioral experiments. by Deacon, R

“…Moreover, particularly when testing novel genetically modified animals or pharmacological agents, there is no substitute for direct visual observation to detect abnormal signs in the animals: for example, ptosis, piloerection, tremor, ataxia or exophthalmos. …”

Burrowing in rodents: a sensitive method for detecting behavioral dysfunction. by Deacon, R

“…The simplicity, sensitivity and robustness of burrowing make it ideal for assessing genetically modified animals, which in most cases would be mice. …”

Adipose tissue: a key target for diabetes pathophysiology and treatment? by Frayn, K, Tan, G, Karpe, F

“…If the normal mechanisms regulating adipocyte fat storage are interfered with (either in genetically-modified animals or by increasing the size of the adipocytes), then perhaps some sort of cellular stress sets in, leading to the inflammatory and endocrine changes. …”

Nociceptive sensitizers are regulated in damaged joint tissues, including the articular cartilage, when osteoarthritic mice display pain behaviour by Driscoll, C, Chanalaris, A, Knights, C, Ismail, H, Sacitharan, P, Gentry, C, Bevan, S, Vincent, A

“…Cartilage injury assays were performed using avulsed immature murine hips from wild type or genetically modified animals, or by explanting articular cartilage from porcine joints pre-injected with pharmacological inhibitors. …”