Rapid characterisation of hERG channel kinetics II: temperature dependence by Lei, CL, Clerx, M, Beattie, KA, Melgari, D, Hancox, JC, Gavaghan, DJ, Polonchuk, L, Wang, K, Mirams, GR

“…In this Part II, we use this protocol to study the temperature dependence of hERG kinetics using Chinese hamster ovary cells overexpressing hERG1a on the Nanion SyncroPatch 384PE, a 384-well automated patch-clamp platform, with temperature control. …”

Contributions of HERG K+ current to repolarization of the human ventricular action potential. by Fink, M, Noble, D, Virag, L, Varro, A, Giles, W

“…For these reasons we and others have attempted to define the functional role for HERG-mediated K+ currents in repolarization of the action potential in the human ventricle. …”

Rapid characterisation of hERG channel kinetics I: using an automated high-throughput system by Lei, CL, Clerx, M, Gavaghan, D, Polonchuk, L, Mirams, GR, Wang, K

“…We demonstrate its use on the Nanion SyncroPatch 384PE, a 384-well automated patch-clamp platform, by applying it to Chinese hamster ovary cells stably expressing hERG1a. From these recordings, we construct 124 cell-specific variants/parameterizations of a hERG model at 25°C. …”

Modelling effects of Sotalol on Action Potential morphology using a novel Markov model of the HERG channel by Brennan, T, Fink, M, Rodriguez, B, Tarassenko, L

The faint source population at 15.7 GHz – III. A high-frequency study of HERGs and LERGs by Whittam, I, Riley, J, Green, D, Jarvis, M

“…Optical spectra are used to classify 17 of the sources as high-excitation or low-excitation radio galaxies (HERGs and LERGs, respectively), for the remaining sources three other methods are used; these are optical compactness, X-ray observations and mid-infrared colour-colour diagrams. 32 sources are HERGs and 35 are LERGs while the remaining 29 sources could not be classified. …”

Application of Human Stem Cell−derived Cardiomyocytes in Safety Pharmacology Requires Caution Beyond hERG by Jonsson, M, Vos, M, Mirams, G, Duker, G, Sartipy, P, de Boer, T, van Veen, T

“…The only compound that triggered EADs was hERG blocker Cisapride. Computer simulations and APclamp showed that the immature AP of hESC-CM prevents proper functioning of INa-channels, and result in lower peak/maximal currents of several other channels, compared to the adult situation. …”

Application of human stem cell-derived cardiomyocytes in safety pharmacology requires caution beyond hERG by Jonsson, M, Vos, M, Mirams, G, Duker, G, Sartipy, P, De Boer, T, Van Veen, T

“…The only compound that triggered EADs was hERG blocker Cisapride. Computer simulations and AP clamp showed that the immature AP of hESC-CM prevents proper functioning of I Na-channels, and result in lower peak/maximal currents of several other channels, compared to the adult situation. …”

Application of human stem cell-derived cardiomyocytes in safety pharmacology requires caution beyond hERG. by Jonsson, M, Vos, M, Mirams, G, Duker, G, Sartipy, P, de Boer, T, van Veen, T

“…The only compound that triggered EADs was hERG blocker Cisapride. Computer simulations and AP clamp showed that the immature AP of hESC-CM prevents proper functioning of I(Na)-channels, and result in lower peak/maximal currents of several other channels, compared to the adult situation. …”

The faint source population at 15.7 GHz - III. A high-frequency study of HERGs and LERGs by Whittam, I, Riley, J, Green, D, Jarvis, M

“…Optical spectra are used to classify 17 of the sources as high-excitation or low-excitation radio galaxies (HERGs and LERGs respectively), for the remaining sources three other methods are used; these are optical compactness, X-ray observations and mid-infrared colour--colour diagrams. 32 sources are HERGs and 35 are LERGs while the remaining 29 sources could not be classified. …”

Importance of modelling hERG binding in predicting drug-induced action potential prolongations for drug safety assessment by Farm, HJ, Clerx, M, Cooper, F, Polonchuk, L, Wang, K, Gavaghan, DJ, Lei, CL

“…Here, we study the impact of including state-dependent drug binding in a mathematical model of hERG when translating hERG inhibition to action potential changes. …”

The link between accretion mode and environment in radio-loud active galaxies by Ineson, J, Croston, J, Hardcastle, M, Kraft, R, Evans, D, Jarvis, M

“…Comparing the samples, we found that although the maximum environment richness of the HERG environments is similar in both samples, there are poorer HERG environments in the z~0.1 sample than in the z~0.5 sample. …”

Simulation of multiple ion channel block provides improved early prediction of compounds' clinical torsadogenic risk by Mirams, G, Cui, Y, Sher, A, Fink, M, Cooper, J, Heath, B, McMahon, N, Gavaghan, D, Noble, D

“…While avoiding the use of drugs with maximum therapeutic concentrations within 30-fold of their hERG inhibitory concentration 50% (IC50) values has been suggested, there are drugs that are exceptions to this rule: hERG inhibitors that do not cause TdP, and drugs that can cause TdP but are not strong hERG inhibitors. …”

Simulation of multiple ion channel block provides improved early prediction of compounds' clinical torsadogenic risk. by Mirams, G, Cui, Y, Sher, A, Fink, M, Cooper, J, Heath, B, McMahon, N, Gavaghan, D, Noble, D

“…While avoiding the use of drugs with maximum therapeutic concentrations within 30-fold of their hERG inhibitory concentration 50% (IC(50)) values has been suggested, there are drugs that are exceptions to this rule: hERG inhibitors that do not cause TdP, and drugs that can cause TdP but are not strong hERG inhibitors. …”

The Stripe 82 1–2 GHz Very Large Array Snapshot Survey: host galaxy properties and accretion rates of radio galaxies by Whittam, I, Prescott, M, McAlpine, K, Jarvis, M, Heywood, I

“…Optical spectra are used to classify these sources as high excitation and low excitation radio galaxies (HERGs and LERGs), resulting in 60 HERGs, 149 LERGs, and 600 ‘probable LERGs’. …”

The radio galaxy population in the SIMBA simulations by Thomas, N, Jarvis, MJ, Davé, R, Anglés-Alcázar, D

“…SIMBA thus predicts that deeper radio surveys will reveal an increasing overlap between the host galaxy demographics of HERGs and LERGs.…”

GAMA/WiggleZ: The 1.4GHz radio luminosity functions of high- and low-excitation radio galaxies and their redshift evolution to z=0.75 by Pracy, M, Ching, J, Sadler, E, Croom, S, Baldry, I, Bland-Hawthorn, J, Brough, S, Brown, M, Couch, W, Davis, T, Drinkwater, M, Jarvis, M, Jelliffe, B, Jurek, R, Loveday, J, Pimbblet, K, Prescott, M, Wisnioski, E, Woods, D

“…The radio AGN are separated into low-excitation radio galaxies (LERGs) and high-excitation radio galaxies (HERGs) using the optical spectra. We derive radio luminosity functions for LERGs and HERGs separately in the three redshift bins (0.005 < z < 0.3, 0.3 < z < 0.5 and 0.5 < z < 0.75). …”

MIGHTEE: the nature of the radio-loud AGN population by Whittam, IH, Jarvis, MJ, Hale, CL, Prescott, M, Morabito, LK, Heywood, I, Adams, NJ, Afonso, J, An, F, Ao, Y, Bowler, RAA, Collier, JD, Deane, RP, Delhaize, J, Frank, B, Glowacki, M, Hatfield, PW, Maddox, N, Marchetti, L, Matthews, AM, Prandoni, I, Randriamampandry, S, Randriamanakoto, Z, Smith, DJB, Taylor, AR, Thomas, NL, Vaccari, M

“…We find no significant difference in the host galaxy properties of the HERGs and LERGs in our sample. In contrast to previous work, we find that the HERGs and LERGs have very similar Eddington-scaled accretion rates; in particular we identify a population of very slowly accreting AGNs that are formally classified as HERGs at these low radio luminosities, where separating into HERGs and LERGs possibly becomes redundant. …”

Early afterdepolarisation tendency as a simulated pro-arrhythmic risk indicator by McMillan, B, Gavaghan, D, Mirams, G

“…Drugs which cause TdP block the hERG cardiac potassium channel. However, not all drugs that block hERG cause TdP. …”

The Stripe 82 1-2 GHz Very Large Array Snapshot Survey: multiwavelength counterparts by Prescott, M, Whittam, I, Jarvis, M, McAlpine, K, Richter, L, Fine, S, Mauch, T, Heywood, I, Vaccari, M

“…For the objects with spectroscopy, we separate radio-loud active galactic nuclei (AGN) and star-forming galaxies (SFGs) using three diagnostics and then further divide our radio-loud AGN into the high and low excitation radio galaxy (HERG and LERG) populations. A control-matched sample of HERGs and LERGs, matched on stellar mass, redshift, and radio luminosity, reveals that the host galaxies of LERGs are redder and more concentrated than HERGs. …”

Computational assessment of drug−induced effects on the electrocardiogram: from ion channel block to body surface potentials by Zemzemi, N, Bernabeu, M, Saiz, J, Cooper, J, Pathmanathan, P, Mirams, G, Pitt−Francis, J, Rodriguez, B

“…<strong>Key results</strong> Introducing a 50% hERG block results in 7.8% prolongation of the APD90 and 5.7% QT interval prolongation, whereas the hERG block does not affect the QRS interval. …”