The murine lung as a factory to produce secreted intrapulmonary and circulatory proteins by Paul-Smith, M, Pytel, K, Gelinas, J, McIntosh, J, Pringle, I, Davies, L, Chan, M, Meng, C, Bell, R, Cammack, L, Moran, C, Cameron, L, Inoue, M, Tsugumine, S, Hironaka, T, Gill, D, Hyde, S, Nathwani, A, Alton, E, Griesenbach, U

“…We assessed whether rSIV.F/HN transduction of the lung generates therapeutically relevant levels of secreted proteins in the lung and systemic circulation using human α1-anti-trypsin (hAAT) and factor VIII (hFVIII) as exemplars. Sedated mice were transduced with rSIV.F/HN carrying either the secreted reporter gene Gaussia luciferase or the hAAT or hFVIII cDNAs by nasal sniffing. rSIV.F/HN-hAAT transduction lead to therapeutically relevant hAAT levels (70 μg/ml) in epithelial lining fluid, with stable expression persisting for at least 19 months from a single application. …”