Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022 by Harvey, DJ

“…The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. …”

Serum protein profiling by miniaturized solid-phase extraction and matrix-assisted laser desorption/ionization mass spectrometry. by Callesen, A, Mohammed, S, Bunkenborg, J, Kruse, T, Cold, S, Mogensen, O, Christensen, R, Vach, W, Jørgensen, P, Jensen, O

“…Serum profiling by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) holds promise as a clinical tool for early diagnosis of cancer and other human diseases. …”

Use of Edman degradation sequence analysis and matrix-assisted laser desorption/ionization mass spectrometry in designing substrates for matrix metalloproteinases. by Lauer-Fields, J, Nagase, H, Fields, G

“…Edman degradation sequence and matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) analyses of proteolytic activity have been utilized to aid in further substrate design. …”

A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry. by Yan, J, Wait, R, Berkelman, T, Harry, R, Westbrook, J, Wheeler, C, Dunn, M

“…We show that tryptic digests of proteins visualized by the modified stain afford excellent mass spectra by both matrix-assisted laser desorption/ionization and tandem electrospray ionization. …”

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia species. by Suttisunhakul, V, Pumpuang, A, Ekchariyawat, P, Wuthiekanun, V, Elrod, M, Turner, P, Currie, B, Phetsouvanh, R, Dance, D, Limmathurotsakul, D, Peacock, S, Chantratita, N

“…Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. …”

Integrated selective enrichment target--a microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases. by Ekström, S, Wallman, L, Malm, J, Becker, C, Lilja, H, Laurell, T, Marko-Varga, G

“…The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. …”

Whole cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of Leptospira spp. in Thailand and Lao PDR by Sonthayanon, P, Jaresitthikunchai, J, Mangmee, S, Thiangtrongjit, T, Wuthiekanun, V, Amornchai, P, Newton, P, Phetsouvanh, R, Day, N, Roytrakul, S

“…This study used matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as rapid and accurate tool for the identification of leptospires. …”

Global proteomic analysis of plasma from mice infected with Plasmodium berghei ANKA using two dimensional gel electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry. by Gitau, E, Kokwaro, G, Newton, C, Ward, SA

A randomised controlled trial of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDITOF-MS) versus conventional microbiological methods for identifying pathogens: Impact on optimal antimicrobial therapy of invasive bacterial and fungal infections in Vietnam by Nadjm, B, Dat, VQ, Campbell, JI, Dung, VTV, Torre, A, Tu, NTC, Van, NTT, Trinh, DT, Lan, NPH, Trung, NV, Hang, NTT, Hoi, LT, Baker, S, Wolbers, M, Chau, NVV, Van Kinh, N, Thwaites, GE, Van Doorn, HR, Wertheim, HFL

A miniaturized screen for inhibitors of Jumonji histone demethylases. by Sakurai, M, Rose, N, Schultz, L, Quinn, A, Jadhav, A, Ng, S, Oppermann, U, Schofield, C, Simeonov, A

Subjects: “…Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization…”

Selective inhibitors of the JMJD2 histone demethylases: combined nondenaturing mass spectrometric screening and crystallographic approaches. by Rose, N, Woon, E, Kingham, G, King, O, Mecinović, J, Clifton, I, Ng, S, Talib-Hardy, J, Oppermann, U, McDonough, M, Schofield, C

Subjects: “…Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization…”

Insulated molecular wires: Synthesis of conjugated polyrotaxanes by Suzuki coupling in water by Taylor, P, O'Connell, M, McNeill, L, Hall, M, Aplin, RT, Anderson, H

“…The matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrum shows a series of oligomers, up to a [10]rotaxane.…”

Application of a proteolysis/mass spectrometry method for investigating the effects of inhibitors on hydroxylase structure. by Stubbs, C, Loenarz, C, Mecinović, J, Yeoh, K, Hindley, N, Liénard, B, Sobott, F, Schofield, C, Flashman, E

“…Limited proteolysis coupled to matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analyses can be used to screen for compounds that alter protein structure by monitoring stabilizing/destabilizing effects with respect to the rate and nature of proteolysis. …”

Preparation and characterisation of high-density ionic liquids incorporating halobismuthate anions. by Cousens, N, Taylor Kearney, L, Clough, M, Lovelock, K, Palgrave, R, Perkin, S

“…The neat ionic liquids and their mixtures with 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide were characterised using Densitometry, Viscometry, NMR Spectroscopy, Electrospray Ionisation Mass Spectrometry (ESI), Liquid Secondary Ion Mass Spectrometry (LSIMS), Matrix-assisted Laser Desorption/Ionization Mass Spectrometry (MALDI), X-Ray Photoelectron Spectroscopy (XPS) and Thermogravimetric Analysis (TGA), to establish their speciation and suitability for high-temperature applications.…”

Characterization of a novel peptide from pathogenic Leptospira and its cytotoxic effect by Paratsaphan, S, Moonsom, S, Reamtong, O, Roytrakul, S, Wuthiekanun, V, Sonthayanon, P, Day, NPJ

“…A specific peptide at a mass-to-charge ratio of 7000 Da was identified in Leptospira whole cells using matrix-assisted laser/desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. …”

Conservation of the function counts: homologous neurons express sequence-related neuropeptides that originate from different genes. by Neupert, S, Huetteroth, W, Schachtner, J, Predel, R

“…By means of single-cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry, we analysed neuropeptide expression in all FXPRLamide/pheromone biosynthesis activating neuropeptide synthesizing neurons of the adult tobacco hawk moth, Manduca sexta. …”

A proteomic investigation of isolated soy proteins with variable effects in experimental and clinical studies. by Gianazza, E, Eberini, I, Arnoldi, A, Wait, R, Sirtori, C

“…After two-dimensional electrophoresis, identities of the protein components (globulin subunits and their breakdown products) were established by matrix-assisted laser desorption/ionization mass spectrometry. The soy concentrates (Cholsoy/Croksoy), which were used in most of the Italian and Swiss studies in which reductions in cholesterolemia occurred, exhibited a predominance of breakdown products of the 7S globulin and mainly intact 11S globulin subunits. …”

Separation and identification of rat skeletal muscle proteins using two-dimensional gel electrophoresis and mass spectrometry. by Yan, J, Harry, R, Wait, R, Welson, S, Emery, P, Preedy, V, Dunn, M

“…We used immobilized pH gradients of 3-10 (non-linear) and 4-7 (linear), and matrix assisted laser desorption/ionization--time of flight mass spectrometry for protein identification by peptide mass fingerprinting. …”

Relationships between the N-glycan structures and biological activities of recombinant human erythropoietins produced using different culture conditions and purification procedures... by Yuen, C, Storring, P, Tiplady, R, Izquierdo, M, Wait, R, Gee, C, Gerson, P, Lloyd, P, Cremata, J

“…The N-glycan structures of these EPOs were analysed using a recently developed profiling procedure and identified using matrix-assisted laser desorption ionization mass spectrometry. The specific activities of each of the EPOs were estimated by in vivo and in vitro mouse bioassays. …”

Analysis of the Arabidopsis mitochondrial proteome. by Millar, A, Sweetlove, L, Giegé, P, Leaver, C

“…A total of 170 protein spots were excised, trypsin-digested, and matrix-assisted laser desorption ionization/time of flight mass spectrometry spectra obtained. …”