From chemical mutagenesis to post-expression mutagenesis: A 50 year odyssey. by Wright, T, Vallée, M, Davis, B

“…Site-directed (gene) mutagenesis has been the most useful method available for the conversion of one amino acid residue of a given protein into another. …”

Structural basis of DNA mutagenesis by Brown, T, Kennard, O

“…Finally, an important study of a dodecamer containing a sequence corresponding to a hotspot for frameshift mutagenesis is described. This structure contains a novel type of groove backbone interaction and novel base mispairing which suggest a molecular model for frameshift substitution mutagenesis.…”

"Site-selected" transposon mutagenesis of Drosophila. by Kaiser, K, Goodwin, S

“…There are obvious implications of this technique for the mutagenesis of other organisms.…”

ENU mutagenesis: Unpicking the immune system by Cornall, R

Bacterial phenotypic heterogeneity in DNA repair and mutagenesis by Vincent, MS, Uphoff, S

Evaluation of neurological mouse mutants caused by ENU mutagenesis by Isaacs, A, Potter, A, Masih, M, Vizor, L, Oliver, P, Davies, K, Peters, J, Nolan, P, Martin, J, Walsh, F, Brown, S, Hunter, A

New insights into behaviour using mouse ENU mutagenesis. by Oliver, P, Davies, K

“…Many human variants causing behavioural disorders are not gene deletions but changes in levels of expression or activity of a gene product; consequently, large-scale mouse ENU mutagenesis has the advantage over the study of null mutants in that it generates a range of point mutations that frequently mirror the subtlety and heterogeneity of human genetic lesions. …”

Analysis of human neurological disorders using mutagenesis in the mouse. by Oliver, P, Davies, K

“…Advances in gene targeting technologies have facilitated the efficiency of generating new mouse mutants, although this valuable resource has rapidly expanded in recent years due to a number of major random mutagenesis programmes. The phenotype-driven mutagenesis screen at the MRC Mammalian Genetics Unit has generated a significant number of mice with potential neurological defects, and our aim has been to characterize selected mutants on a pathological and molecular level. …”

Single-molecule analysis of bacterial DNA repair and mutagenesis by Uphoff, S, Sherratt, D

“…Furthermore, unexpected discoveries have resulted from capabilities to resolve molecular heterogeneity and cell subpopulations, provoking new questions about the role of stochastic processes in DNA repair and mutagenesis. These studies are leading to new technologies that will find widespread use in basic research, biotechnology, and medicine.…”

Evaluating evolutionary models of stress-induced mutagenesis in bacteria by MacLean, R, Torres-Barceló, C, Moxon, R

“…Here we evaluate existing models for the evolution of stress-induced mutagenesis and present a new model arguing that it evolves as a result of a complex interplay between direct selection for increased stress tolerance, second-order selection for increased evolvability and genetic drift. …”

High throughput in vivo screens: Signature-tagged mutagenesis by West, N, Sansonetti, P, Tang, C

Evaluating evolutionary models of stress-induced mutagenesis in bacteria. by Maclean, C, Torres-Barceló, C, Moxon, R

“…Here we evaluate existing models for the evolution of stress-induced mutagenesis and present a new model arguing that it evolves as a result of a complex interplay between direct selection for increased stress tolerance, second-order selection for increased evolvability and genetic drift. …”

Tagged mutagenesis by efficient Minos-based germ line transposition. by de Wit, T, Dekker, S, Maas, A, Breedveld, G, Knoch, T, Langeveld, A, Szumska, D, Craig, R, Bhattacharya, S, Grosveld, F, Drabek, D

Mutagenesis studies on the iron binding ligands of clavaminic acid synthase. by Doan, L, Hassan, A, Lipscomb, S, Dhanda, A, Zhang, Z, Schofield, C

“…Mutagenesis studies on conserved histidine residues identified as possible metal binding ligands in clavaminic acid synthase isozyme 2 were consistent with His-145 and His-280 acting as iron ligands, in support of crystallographic and previous mutagenesis studies. …”

Signature-tagged mutagenesis: barcoding mutants for genome-wide screens. by Mazurkiewicz, P, Tang, C, Boone, C, Holden, D

Site-directed mutagenesis of beta-lactamase I: role of Glu-166. by Leung, Y, Robinson, C, Aplin, RT, Waley, S

Meiotic DNA breaks drive multifaceted mutagenesis in the human germ line. by Hinch, R, Donnelly, P, Hinch, AG

“…We provide evidence that these mechanisms drive mutagenesis in human germ lines and lead to disruption of hundreds of genes genome wide.…”

Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system by Bassett, AR, Tibbit, C, Ponting, CP, Liu, J-L

“…We show that injection of RNA into the Drosophila embryo can induce highly efficient mutagenesis of desired target genes in up to 88% of injected flies. …”

Interactions between residues in staphylococcal alpha-hemolysin revealed by reversion mutagenesis. by Panchal, R, Bayley, H

“…The present study suggests that the key N-terminal residue His-35 operates in conjunction with residues flanking the loop and C-terminal residues in the region 217-228. Hence, reversion mutagenesis extends the linear analysis that has been provided by direct point mutagenesis.…”

DNA Replication and associated repair pathways are involved in the mutagenesis of methylated cytosine by Tomkova, M, McClellan, M, Kriaucionis, S, Schuster-Böckler, P

“…Together, these findings suggest that mis-incorporation of A opposite 5mC during replication of the leading strand might be a contributing factor in the mutagenesis of methylated cytosine.…”