Genetics of reactive arthritis by Chan, A, Wordsworth, P

Multiple T cell expansions are found in the blood and synovial fluid of patients with reactive arthritis. by Allen, R, Gillespie, G, Hall, F, Edmonds, S, Hall, M, Wordsworth, B, McMichael, A, Bowness, P

“…OBJECTIVE: To look for evidence of T lymphocyte expansions in the blood and synovial fluid (SF) of patients with reactive arthritis (ReA). METHODS: Paired peripheral blood and synovial samples from 10 patients with ReA were studied by dual color flow cytometry using T cell receptor (TCR) V beta specific and CD4 or CD8 specific antibodies. …”

HLA-B27-RESTRICTED PEPTIDE PRESENTATION TO CYTOTOXIC T-CELLS IN REACTIVE ARTHRITIS by Bowness, P, Allen, R, Mcmichael, A, Edmunds, S, Hall, M, Bowman, S, Wordsworth, B

Collagen-specific cytotoxic T lymphocyte responses in patients with ankylosing spondylitis and reactive arthritis. by Gao, X, Wordsworth, P, McMichael, A

“…Both ankylosing spondylitis (AS) and reactive arthritis (ReA) are strongly associated with HLA-B27 although the mechanism for this association is still unknown. …”

T-CELL RECEPTOR USAGE OF SYNOVIAL LYMPHOCYTES FROM A PATIENT WITH REACTIVE ARTHRITIS by Bowness, P, Wordsworth, B, Moss, P, Mcmichael, A, Bell, J

Chlamydial DNA is absent from the joints of patients with sexually acquired reactive arthritis. by Wordsworth, B, Hughes, R, Allan, I, Keat, A, Bell, J

“…In contrast, chlamydial DNA was not detectable in the knee joints of nine patients with definite sexually acquired reactive arthritis (SARA) or nine patients with suspected SARA. …”

MLST clustering of Campylobacter jejuni isolates from patients with gastroenteritis, reactive arthritis and Guillain-Barre syndrome. by Nielsen, L, Sheppard, S, McCarthy, N, Maiden, M, Ingmer, H, Krogfelt, K

“…AIMS: To determine the diversity and population structure of Campylobacter jejuni (C. jejuni) isolates from Danish patients and to examine the association between multilocus sequence typing types and different clinical symptoms including gastroenteritis (GI), Guillain-Barré syndrome (GBS) and reactive arthritis (RA). METHODS AND RESULTS: Multilocus sequence typing (MLST) was used to characterize 122 isolates, including 18 from patients with RA and 8 from patients with GBS. …”

Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis. by Appel, H, Kuon, W, Kuhne, M, Wu, P, Kuhlmann, S, Kollnberger, S, Thiel, A, Bowness, P, Sieper, J

“…The application of HLA-B27 tetramers in HLA-B27-related diseases was performed with nine recently described Chlamydia-derived peptides in synovial fluid and peripheral blood, to examine the CD8+ T cell response against Chlamydia trachomatis antigens in nine patients with Chlamydia-triggered reactive arthritis (Ct-ReA). Four of six HLA-B27+ Ct-ReA patients had specific synovial T cell binding to at least one HLA-B27/Chlamydia peptide tetramer. …”

Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis. by Appel, H, Kuon, W, Kuhne, M, Wu, P, Kuhlmann, S, Kollnberger, S, Thiel, A, Bowness, P, Sieper, J

“…The application of HLA-B27 tetramers in HLA-B27-related diseases was performed with nine recently described Chlamydia-derived peptides in synovial fluid and peripheral blood, to examine the CD8+ T cell response against Chlamydia trachomatis antigens in nine patients with Chlamydia-triggered reactive arthritis (Ct-ReA). Four of six HLA-B27+ Ct-ReA patients had specific synovial T cell binding to at least one HLA-B27/Chlamydia peptide tetramer. …”

Conserved TCR beta chain usage in reactive arthritis; evidence for selection by a putative HLA-B27-associated autoantigen. by May, E, Dulphy, N, Frauendorf, E, Duchmann, R, Bowness, P, Lopez de Castro, J, Toubert, A, Märker-Hermann, E

“…Previous work suggested that expanded CD8+ T-cell clones in the synovial fluid (SF) of HLA-B27+ patients with reactive arthritis (ReA) preferentially use the T-cell receptor variable region (TCRBV) 1, similar CDR3 sequences, and joining region (BJ) 2S3. …”

Increased expression of HLA-DQ antigens by interstitial cells and endothelium in the synovial membrane of rheumatoid arthritis patients compared with reactive arthritis patients. by Barkley, D, Allard, S, Feldmann, M, Maini, R

“…We investigated cellular phenotypes and expression of class II major histocompatibility complex antigens on endothelium and cellular infiltrates in synovium from patients with rheumatoid arthritis (RA) or reactive arthritis, using an indirect immunoperoxidase technique. …”

Homocysteine modification of HLA antigens and its immunological consequences. by Gao, X, Wordsworth, P, Mcmichael, A, Kyaw, M, Seifert, M, Rees, D, Dougan, G

“…Homocysteine-treated cells can be specifically lysed by cytotoxic T lymphocytes (CTL) identifiable in patients with ankylosing spondylitis and reactive arthritis. Sensitization of target cells involves disulfide bonding and the interaction between homocysteine and HLA antigens occurs in a pre-Golgi compartment in the cells. …”

Identification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases. by Kuon, W, Holzhütter, H, Appel, H, Grolms, M, Kollnberger, S, Traeder, A, Henklein, P, Weiss, E, Thiel, A, Lauster, R, Bowness, P, Radbruch, A, Kloetzel, P, Sieper, J

“…The association of HLA-B27 with ankylosing spondylitis and reactive arthritis is the strongest one known between an MHC class I Ag and a disease. …”

Immunogenetics and rheumatic diseases: 25 years on from HLA-B27 by Wordsworth, P

“…. · The function of HLA molecules in the recognition of viral and bacterial antigens is now well described and has important implications for the pathogenesis of diseases such as ankylosing spondylitis, reactive arthritis and rheumatoid arthritis. · HLA-B27 is strongly associated with ankylosing spondylitis and has played an important role in defining a group of related spondyloarthropathies. · HLA genes contribute less than half of the genetic basis of these diseases. · Whole genome screening is a power tool to detect other genes relevant to these conditions.…”

Enteropathic arthritis. by Holden, W, Orchard, T, Wordsworth, P

“…This form of arthritis is classified as one of the group of seronegative spondyloarthropathies, which also includes psoriatic arthritis, reactive arthritis, and idiopathic ankylosing spondylitis. …”

Spondyloarthropathy in vertebrae of the aquatic Cretaceous snake Lunaophis aquaticus, and its first recognition in modern snakes by Albino, A, Rothschild, B, Carrillo-Briceño, J, Neenan, J

“…The pathology likely represents a form of reactive arthritis related to enteropathic infection. While the disease probably did not affect general locomotion, its vertebral column position may have compromised mating.…”

TCR usage by homocysteine-specific human CTL. by Chilvers, M, Wordsworth, P, Stubbs, A, Gao, X

“…Here, we have investigated TCR usage by Hom-CTL from five patients with ankylosing spondylitis or reactive arthritis. TCR of HLA-A68-restricted Hom-CTL from two unrelated donors share the same TCR Valpha, Vbeta, and Jbeta gene segments (AV4, BV23, and BJ2S1, respectively) with similar third complementarity determining regions (CDR3) of the beta-chains. …”

Genetic susceptibility to enteric fever in experimentally challenged human volunteers by Barton, A, Hill, J, Bibi, S, Chen, L, Jones, C, Jones, E, Camara, S, Shrestha, S, Jin, C, Gibani, MM, Dobinson, H, Waddington, C, Darton, TC, Blohmke, CJ, Pollard, AJ

“…Imputation of classical human leukocyte antigen (HLA) types from genomic and transcriptomic data identified HLA-B*27:05, previously associated with nontyphoidal Salmonella-induced reactive arthritis, as the HLA type most strongly associated with enteric fever susceptibility (P = 0.011). …”

Stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis by Choi, I, Karpus, O, Turner, J, Hardie, D, Marshall, J, de Hair, M, Maijer, K, Tak, P, Raza, K, Hamann, J, Buckley, C, Gerlag, D, Filer, A

“…Diagnostic classification (gout, psoriatic arthritis, unclassified arthritis (UA), parvovirus associated arthritis, reactive arthritis and RA), disease outcome (resolving vs persistent) and clinical variables were determined at baseline and after follow-up, and related to the expression of stromal markers. …”

Consistent patterns of expression of HLA class I free heavy chains in healthy individuals and raised expression in spondyloarthropathy patients point to physiological and pathologi... by Raine, T, Brown, D, Bowness, P, Hill Gaston, J, Moffett, A, Trowsdale, J, Allen, R

“…FHCs were present at higher levels in a reactive arthritis (ReA) population than in healthy controls and RA patients; differences not merely due to the presence of Human Leucocyte Antigen (HLA) B27. …”