Method of recovering glycoproteins and/or proteins derived from edible birds’s nest by Tan, Chin Ping, Lim, Hong Kwong, Ling, Tau Chuan, Abas, Faridah, Ng, Siou Pei

“…The present invention discloses a method of recovering glycoproteins and/or proteins from edible bird’s nest (EBN) of AerodramusandCollocaliaspp, which comprises the steps of preparing a crude extract of the edible bird’s nest and recovering glycoproteins and/or proteins contained in the crude extract by way of an aqueous two-phase system. …”

An improved method for the detection of white spot syndrome virus (WSSV) by single-tube nested deoxyribonucleic acid amplification and a detection kit utilizing the method. by Mohamed Din, Mohamed Shariff, Lee, Kok Leong, Soon, Min Ngeng Samson, Tan, Lee Tung

“…The amplification process with these nested primers also reduces background and non-specific amplification. …”

Detection of monodon baculovirus by molecular biology method. by Mohamed Din, Mohamed Shariff, Lee, Kok Leong

“…The development of a nested polymerase chain reaction (PCR) assay is described to detect low concentrations of monodon baculovirus (MBV) DNA. …”

Detection of Newcastle disease virus. by Yusoff, Khatijah, Kho, Chiew Ling, Mohd Lila, Mohd Azmi, Arshad, Siti Suri

“…amplifying the cDNA from step (b) by polymerase chain reaction to form an amplified PCR product (d) converting the amplified PCR product from step ? with a pair of nested primers to form a nested labelled PCR streptavidin-coated microtiter plate; (f) adding a peroxidase substrate to the immobilised product from step (e) to produce a coloured reaction product; and (g) detecting the resulting coloured reaction product using an ELISA reader. …”