Identification and characterization of fungal contamination on spent mushroom substrate by Antong Ibrahim, Nur Natasha

“…Fungal strains were isolated from diseased mushroom bags from mushroom houseUniversiti Putra Malaysia. The pure fungi isolated from spent mushroom substrate were identified by in vitro morphological and molecular characteristics. …”
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Cultivation and identification of selected wild edible mushroom (Lentinus sp.) grown on sawdust substrate by Takril, Nurul-Fatini

“…The wild edible Lentinus were collected along the rivers in Selangor areas. The pure culture was isolated from the fruiting bodies. …”
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Antimicrobial properties of local honey against pathogenic wound causing bacteria in animals by Mussafeer, Jiavendrasingh

“…The well diffusion method was performed to screen pure undiluted honeys against bacteria, and the zones of inhibition were measured. …”
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Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L. by Mohd Din, Siti Norliza

“…Infected tissues (5 x 5mm) from the lesions margin was being surface disinfected for 2 min with 10% Clorox and cultured on potato dextrose agar (PDA). The pure fungal isolates isolated from fruit lesions were identified by conidial and in vitro morphological characteristics according to Mordue et al.,(1971). …”
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Phenotypic and molecular characterization of Pantoea agglomerans causing citrus canker of calamansi (Citrus microcarpa) in Selangor by Fung, Vincent Roderic Jr

“…The pure cultures were yellowish in color, Gram-negative with rod-shaped, positive reaction for catalase test and potassium hydroxide (KOH) tests; and negative for oxidase test. …”
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Morphological and molecular identification characterization of Neopestalotiopsis species associated with leaf spot on oil palm (Elaeis guineensis Jacq) by Norddin, Syahida

“…Infected tissues (5× 5mm) from the lesion margin were surface disinfected for 2 min with 10% chlorox and were transferred on potato dextrose agar (PDA). The pure fungal isolate isolated from leaf lesions were identify by in vitro morphological and cultural characteristics. …”
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A Case Study on Puspamara Sendirian Berhad by Abd Hamid, Abidullah

“…Bearish stock market, depreciation of currency, down-sizing of companies and other economic difficulties have resulted in a violence and pure challenging bussiness environment. These factors awakened the necessity of proper and organized planning and strategy for the company's current and coming survival. …”
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Purification and characterization of recombinant lipase from Arthrobacter sp. 3B by Padrilah, Siti Nadzirah

“…The molecular weight of the pure protein was estimated to be 66.2 kDa by SDS-PAGE. …”
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Plasmid characterization of Enterobacter sp. From human breast milk in Malaysia by Abd Samad, Fatin Illani

“…Extraction method of modified alkaline lysis and using Promega PureYield™ Plasmid Miniprep System were used to obtain high concentration of plasmids. …”
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Effects of reproductive stages on the prevalence of the normal flora in the doe by Ajaman, Norazmanita Edayu

“…Subculture was done to obtain the pure colonies. Gram staining was performed followed by biochemical test accordingly. …”
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Antibacterial activity of locally isolated thermophilic fungi by Abdul Karim, Akmal Hayat

“…Isolation was done and two pure cultures were obtained named CP1 and CP2. Then, it was incubated at three different ranges of temperature to determine the best temperature of fungal growth. …”
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Toxicity evaluation of curcumin analogue on zebrafish by Tan, Hong Wei

“…Curcumin analogues are of great interest in pharmacological research due to its advantages compared with pure curcumin. Curcumin analogues are proposed to be used as an alternative curative drugs for the treatment of certain ailments such as inflammatory diseases.Recently, zebrafish has emerge as a toxicology test model organism for the research of vertebrae genetics and developmental biology. …”
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In vitro acaricidal assessment of betel leaves (Piper betle) aqueous and ethanol extracts on brown dog tick (Rhipicephalus sanguineus) by Shamsi, Muhammad Hafiz

“…Results from both experiments showed significant effect of the betel leaves as acaricide with the lethal dose from one to five dilutions of the pure extract. The ethanol extract have higher efficacy with 26 out of 50 ticks died (52%), meanwhile for the aqueousextract only 8 ticks died out of 50 (16%). …”
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Isolation and characterization of fungal pathogen associated with oil palm black base rot disease by Latif, Nurnashirah

“…Successfully isolated pure cultures were then subjected to cultural and morphological identification based on the demonstrated characteristics. …”
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Isolation, characterization and pathogenicity of fungal pathogens causing post-harvest spoilage in syzygium malaccense (malay apple) by Daud, Alawiah

“…These samples were subjected to isolation step on Potato Dextrose Agar (PDA) prior to cultural and morphological identification based on visibility and microscopic characteristics of each pure culture isolated. This was then followed by molecular identification, where genomic DNA was extracted and amplified using ITS 1 and ITS 4 universal primer sets and the polymerase chain reaction (PCR) products of each isolate subjected to sequencing and later BLAST analysis based on GenBank sequence database. …”
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Pathogenicity study of extracellular proteins (ECPs) and cellular membrane proteins (CMPs) of Streptococcus agalactiae and its effect as immunomodulator in African catfish (Clarias... by Mat Yunus, Nor Aniskiha

“…This study aimed to evaluate the in vivo effect of the ECPs and CMPs of Streptococcus agalactiae on pathogenicity and immunity of the African catfish (Clariasgariepinus) fingerlings.The ECPs and CMPs of S.agalactiae were intraperitoneally (i.p) injected into African catfish fingerlings to determine whether the bacteria products able to cause disease and also stimulate fish‟s immune response. Bacteria cells from pure culture were harvested by centrifugation at 1800xg for 15 minutes to separate ECPs and CMPs from bacteria suspension. …”
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Identification and characterization of fungal species causing sheath blight diseases on paddy ( Oryza sativa L. ) by Mahusin, Mawardi

“…This study were focusing on isolation of fungal pure culture, then identify fungal pathogens to species level based on morphological characteristics and polymerase chain reaction (PCR) protocol using ITS4 and ITS5 primers and also to construct internal transcribed spacer (ITS) phylogeny of the isolated fungal species. …”
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International Strategic Marketing Plan for a Multinational Manufacturing Company by Ng, Jacky Fock Thiam

“…The results of tensile and flexural tests for OPF/Epoxy composites showed that by the addition of fibre content, the tensile and flexural strength was decreased compared to pure epoxy resin (0% fibre). It has been notice that to obtain the optimum tensile and flexural properties was found at the volume fraction of 5%. …”
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Identification and pathogenicity test of Curvularia species causing fruit rot disease on pomelo (Citrus maxima) by Salim, Siti Fairuz

“…The objectives of this study are; 1) to isolate pure culture of fungal isolates causing fruit rot on pomelo; 2) to identify fungal pathogens to species level based on morphological characteristics and polymerase chain reaction (PCR) protocol using ITS4 and ITS5 primers; and 3) to construct a phylogenetic tree using ITS analysis. …”
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Prevalence of Moraxella ovis infection in goats under the Ladang Angkat Programme of University Veterinary Hospital, Universiti Putra Malaysia by Gopi Naidu, Nagachandra Rao

“…Both the subconjuctival swabs and the fly samples were cultured on blood agar and the colonies were screened using Gram staining to isolate the most likely colonies. The pure isolates were then subjected for conventional Polymerase Chain Reaction (PCR) to detect for Moraxella ovis. …”
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