Viable mice with extensive gene humanization (25-kbp) created using embryonic stem cell/blastocyst and CRISPR/zygote injection approaches by Leidy-Davis, Tiffany, Cheng, Kai, Goodwin, Leslie O., Morgan, Judith L., Juan, Wen Chun, Roca, Xavier, Ong, S. Tiong, Bergstrom, David E.

“…Using a unique second method, we employed the same vector (devoid of its selectable marker cassettes), microinjecting it along with redundant single guide RNAs (sgRNAs) and Cas9 mRNA into mouse zygotes (CRISPR/Zygote Approach). In both instances, we were able to achieve humanization of Bcl2l11 to the extent designed, remove all selection cassettes, and demonstrate the functionality of the conditionally removable, loxP-flanked, 2.9-kbp intronic segment.…”
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Micropipette replacement for automated cell micromanipulation by Wijaya, Nico Fendy

“…One of the step in PGD is to change the micropipette in every cycle to avoid cross contamination among different zygote. The objective of this project is to introduce a mechanism to change the pipette and study the advantages and disadvantages of different mechanism that will make up the full automated PGD machine. …”
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Characterization of zebrafish IKK2 mutants generated by zinc finger nucleases by Ho, Foo Kiong

“…Unfortunately, embryonic lethality in IKK2-deficient mice hinders studies of zygotic IKK2 function beyond embryonic stages. Although conditional knockout mice have been used to circumvent this problem, studies using zebrafish as another vertebrate model offer an alternative approach. …”
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Functional characterization of ikk2 zebrafish mutants and TALEN-mediated genetic screen of camel mutants. by Chua, Yun Yu.

“…We found that ikk2 plays both a maternal and zygotic function in early embryonic development. We demonstrate that zygotic function of ikk2 is required during embryogenesis to establish vascular integrity. …”
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Analysis of histone variant H3.3 function in embryonic development and stem cell differentiation by Tai, Joyce An Yi

“…Mutant mRNA H3.3A K4A and H3.3A K27A, which were introduced through microinjection, were overexpressed in mouse zygotes. Preliminary results showed that mutation of H3.3A K27 may delay embryonic development by approximately 1 cleavage-stage while mutation of H3.3A K4 may lead to improper formation of ICM in blastocysts. …”
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Selective binding of retrotransposons by ZFP352 facilitates the timely dissolution of totipotency network by Li, Zhengyi, Xu, Haiyan, Li, Jiaqun, Xu, Xiao, Wang, Junjiao, Wu, Danya, Zhang, Jiateng, Liu, Juan, Xue, Ziwei, Zhan, Guankai, Tan, Bobby Cheng Peow, Chen, Di, Chan, Yun-Shen, Ng, Huck Hui, Liu, Wanlu, Hsu, Chih-Hung, Zhang, Dan, Shen, Yang, Liang, Hongqing

“…Currently, extensive insights have been revealed for the totipotency regulatory network around the zygotic genome activation (ZGA) period. However, how the dissolution of the totipotency network is triggered to ensure the timely embryonic development following ZGA is largely unknown. …”
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Characterization of Creb-regulated transcription coactivator 1 (Crtc1) during embryogenesis of Zebrafish (Danio rerio) by Sie, Lawrence Eng Kean

“…I have characterized the spatial and temporal expression of crtc1 and crtc1b during zebrafish development, and identified unique splicing of maternal and zygotic specific crtc1b transcripts. Also, I have demonstrated the co-activator function of zebrafish Crtc1 and Crtc1b by using CRE luciferase reporter assay in cell culture. …”
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