Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method by Jacinta Santhanam, Siti Azura Zainon, Chin, Chook Fung, Faezah Shekh Abdullah

“…Diagnostic procedures involving culture or serological tests are either slow or lacking sensitivity and specificity. Thus polymerase chain reaction (PCR) assays which detect fungal DNA have been developed to provide a rapid, accurate diagnosis. …”
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Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features by Said Saleh, King, Alfred Yin Lam, Yik, Hong Ho

“…Human survivin mRNA levels were quantitatively measured by real time polymerase chain reaction (PCR) in tumor and adjacent non-tumor tissues. …”
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Polymerase chain reaction analysis as a rapid screening test for diagnosis of fragile X syndrome by Phan CL, Zubaidah Z, Gregory A.R.A, Kamariah MN, Ten SK, Maimonah R, Thilagavathi S

“…This study was aimed to establish a rapid polymerase chain reaction (PCR) based - touch down PCR, as a screening method for fragile X syndrome. …”
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Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification by Lim, Jonathan Jun-Yong, Mohd Fadhli Khamis, Nur Haslindawaty Abd Rashid

“…This study aims to ascertain the sex of an individual from burnt teeth samples exposed at different temperature and time through nested polymerase chain reaction (PCR) on the amelogenin (AMEL) sex marker, to calculate the specificity and sensitivity, and to compare with previous relevant studies. …”
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Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis by Safiyyah Shahimi, Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Aminah Abdullah, Norrakiah Abdullah Sani

“…Genomic DNA of 13 fish (n=13) species consist of four freshwater which were catfish (Clarias gariepinus), shark catfish (Pangasius larnaudii), tilapia (Oreochromis mossambicus), perch (Lates calcarifer) and nine marine species which were black pomfret (Parastromateus niger), anchovy (Stolephorus commersonii), mabong (Rastrelliger kanagurta), red snapper (Lutjanus erythropterus), herring (Chirocentrus dorab), ray fish (Himantura gerrardii), sardine (Decapterus macrosoma), mackerel (Euthynnus affinis) and tuna (Thunnus tuna) were differentiated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). …”
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Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques by Reena RMZ, Julia Munchar MJ, Salwati S, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK

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Detection of transovarial dengue viruses in Aedes albopictus from selected localities in Kuching and Samarahan divisions, Sarawak, Malaysia by reverse transcription polymerase chain reaction (RT-PCR) by Nor Aliza Abdul Rahim, Harvie Shukri, Nur Ain Mardiah Rizuan, Lela Suut, Siti Fairouz Ibrahim, Razitasham Safii, Marlini Othman

“…The mosquitoes were screened in the laboratory by using a conventional method of reverse transcription polymerase chain reaction (RT-PCR). Dengue virus serotype 1 (DENV-1) and 2 (DENV-2) were detected in two of the mosquito pools comprising of adult females Ae. albopictus which emerged from the immatures. …”
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Detection of shiga toxin 1 and 2 (stx1 and stx2) genes in escherichia coli 0157: H7 isolated from retail beef in Malaysia by multiplex polymerase chain reaction (PCR) by A.M. Sahilah, H. Nor' Aishah, A. Ahmad Azuhairi

“…Twenty (n=20) beef isolates of Escherichia coli O157:H7 were examined for the detection of Shiga- toxin 1 and 2 (stx1 and stx2) genes by multiplex polymerase chain reaction (PCR) and characterized using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) fingerprinting. …”
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Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP) by Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Safiyyah Shahimi, Norhayati Yaakob, Norrakiah Abdullah Sani, Aminah Abdullah, Abdul Salam Babji, Maaruf Abd. Ghani

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Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia by Rozilawati H., Mohd Masri S, Zairi J., Yahaya M.A., Nazni W.A., Lee, H.L.

“…Reverse transcriptase polymerase chain reaction (RT-PCR) were used as the detection of the virus. …”
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Production of transgenic rice (indica cv. MR219) overexpressing Abp57 Gene through Agrobacterium-mediated transformation by Tan, Lay-Wen, Zuraida A. Rahman, Goh, Hoe-Han, Hwang, Duk-Ju, Ismanizan Ismail, Zamri Zainal

“…The results of polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) indicated that the transgene was successfully integrated and overexpressed in transgenic rice of MR219. …”
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Advances in sago palm research: a comprehensive review of recent findings by Fifi Hafizzah Pendi, Yan, Wei-Jie, Hasnain Hussain, Hairul Azman Roslan, Norzainizul Julaihi

“…This paper provides an overview of the molecular methods previously utilised to decipher the genes responsible conferring the characteristics of sago palm molecular techniques employed to investigate M. sagu molecular properties include molecular markers, genome walking, rapid amplification of cDNA ends (RACE), polymerase chain reaction (PCR) and sequencing. Additionally, this article presents other molecular techniques that can be applied to M. sagu for future crop breeding.…”
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Role of QuantiFERON TB gold test for diagnosis of tuberculosis infection in prosthetic knee joint: a case report by Norliyana M, Nor Hamdan Mohamad Yahaya, Rizal Abd Rani

“…The tuberculin skin test, MTB acid-fast stain and tuberculosis polymerase chain reaction were negative. The diagnosis dilemma was solved with positive result of QuantiFERON TB Gold Test. …”
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Vitreous haemorrhage: a consequence of herpes simplex acute retinal necrosis by Sherina Quay, Rosiah M, Mushawiahti Mustapha

“…Intravenous acyclovir 300mg, 3 times a day was initiated promptly while vitreous fluid was sent for polymerase chain reaction, which identified Herpes Simplex Virus-1. …”
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Phylogenetic analysis of respiratory syncytial virus identified at Universiti Kebangsaan Malaysia Medical Centre by Rahman, M.M., Wong, K.K., Isahak, I., Rashid, Z.Z., Alfizah, H.

“…From a total of 130 throat swab and nasopharyngeal aspirate specimens, 54 (41.5%) were positive with RSV, identified by in-house real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assay. Thirty-four out of 54 (63.0%) RSV positive patients were children below two years old and two (1.4%) were adults. …”
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Effective hygromycin concentration for selection of Agrobacterium-mediated transgenic Arabidopsis thaliana by Ee, S.F., Khairunnisa, M.B., Zeti-Azura, M.H., Noor Azmi, S., Zamri, Z.

“…The identified transgenic seedlings were further verified through polymerase chain reaction (PCR), giving fragment with an expected size of 365 bp.…”
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Study on genetic variability of mud crab, Scylla olivacea at east coast of Peninsular Malaysia using random amplified polymorphic DNA (RAPD): a preliminary assessment by Wan Bayani Wan Omar, Faridah Mohamad, Ikhwanuddin @ Polity Abdullah, Mohd Firdaus Zainal Abidin

“…Thus, the Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique was used to examine the genetic variability among individuals of S. olivacea from Setiu Wetlands, Terengganu. …”
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Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis by Nursheila Mustafa Muin, Sahilah Abd. Mutalib, Aishah Elias, Norrakiah Abdullah Sani, Aminah Abdullah, Razalee Sedek, Izhar Ariff Mohd. Kashim

“…This study was carried out to compare the sensitivity of two detection techniques using conventional polymerase chain reaction (PCR) and Porcine gene chip (OliproTM, Selangor, MY). …”
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A review of mitochondrial SNP determination using allele-specific PCR in forensic identification by Shalini Parthipan, Mohitha Sandrasagran, Seri Mirianti Ishar

“…Allele-specific polymerase chain reaction (AS-PCR) is a rapid and cost-effective single nucleotide polymorphism (SNP) genotyping method compared to multiplex or real-time PCR. …”
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Mycotoxin production by Fusarium proliferatum and Fusarium fujikuroi causing stem rot of hylocereus polyrhizus in Malaysia by Masratul Hawa Mohd, Nik Mohd Izham Mohamed Nor, Nurul Farizah Azuddin, Latiffah Zakaria

“…In this study, we investigated the ability of F. proliferatum and F. fujikuroi to produce FB1 , MON, and BEA. Polymerase chain reaction amplification using FUM1-specific primers detected the gene in all 44 isolates tested, indicating that all isolates produced FB1 . …”
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