Genoprotection and Cytotoxicity of Cassia surattensis Seed Extract on Vero Cell Evaluated by Comet and Cytotoxicity Assays by Uthaya Kumar, U. Seeta, Jothy, Subramanion Lachumy, Kavitha, Nowroji, Chen, Yeng, Kanwar, Jagat Rakesh, Sasidharan, Sreenivasan

“…The seed extract of C. surattensis exhibited strong inhibitory effects against H2O2-mediated DNA damage in Vero cells as observed in Comet assay. The presence of antioxidant activity in the seed extract was associated with the antigenotoxic property of C. surattensis. …”

White tea (Camellia sinensis) inhibits proliferation of the colon cancer cell line, HT-29, activates caspases and protects DNA of normal cells against oxidative damage by Hajiaghaalipour, F., Kanthimathi, M.S., Sanusi, J., Rajarajeswaran, J.

“…DNA damage in 3T3-L1 normal cells was detected by using the comet assay. The extract protected 3T3-L1 cells against H2O2-induced DNA damage. …”

Dietary spices protect against hydrogen peroxide-induced DNA damage and inhibit nicotine-induced cancer cell migration by Jayakumar, R., Kanthimathi, M.S.

“…Murine fibroblasts (3T3-L1) and human breast cancer (MCF-7) cells were pre-treated with spice extracts and then exposed to H 2O 2 and nicotine. The comet assay was used to analyse the DNA damage. Among the 9 spices, ginger, at 50 μg/ml protected against 68 of DNA damage in 3T3-L1 cells. …”

A high antioxidant level in edible plants is associated with genotoxic properties by Wan-Ibrahim, W.I., Sidik, K., Kuppusamy, U.R.

“…Single-cell gel electrophoresis (SCGE) or comet assay was carried out to determine the level of DNA damage in human lymphocytes. …”
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Cell Proliferation and DNA Repair Ability of Ganoderma neo-japonicum (Agaricomycetes): An Indigenous Medicinal Mushroom from Malaysia by Tan, Wee Cheat, Kuppusamy, Umah Rani, Phan, Chia Wei, Sabaratnam, Vikineswary

“…The potential use of G. neo-japonicum in genoprotection and DNA repair was established using a single-cell gel electrophoresis (comet) assay. The effects of the ethanol and hot aqueous extracts from wild and culti-vated basidiocarps, solid substrate-fermented (SSF) wheat grains, and mycelia via submerged culture on H2O2-damaged murine RAW264.7 macrophages were investigated. …”

Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil. by Thirugnanasampandan, R., Ramya, G., Gogulramnath, M., Rajarajeswaran, J., Kanthimathi, M.S.

“…Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthus amboinicus to test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). …”

Synergistic cytotoxic effects of combined δ-tocotrienol and jerantinine B on human brain and colon cancers by Abubakar, I.B., Lim, K.H., Kam, T.S., Loh, H.S.

“…Fluorescence and histochemical staining techniques as well as comet assay were used for evaluating the morphological changes and DNA damage pattern, respectively. …”