Physiological responses of callus from gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation by Hasbullah, N.A., Taha, R.M., Saleh, A., Mohamed, N.

“…In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. …”
Get full text

Dengue Virus Type 2 NS3 Protease and NS2B-NS3 Protease Precursor induce Apoptosis by Shafee, N., AbuBakar, Sazaly

“…NS2B-NS3(185)(pro) was detected as NS2B-NS3(185)(pro)-EGFP fusion protein in cytoplasmic vesicular structures in the apoptotic cells. Site-directed mutagenesis which replaced His 51 with Ala within the protease catalytic triad significantly reduced the ability of the expressed NS3 and NS2B-NS3(185)(pro) to induce apoptosis. …”
Get full text

Functional Role of Tyr12 in the Catalytic Activity of Novel Zeta-like Glutathione S-transferase from Acidovorax sp. KKS102 by Shehu, Dayyabu, Alias, Zazali

“…The functional role of Tyr12 was also investigated by site-directed mutagenesis. The mutant (Y12C) displayed low catalytic activity and dehalogenation function against all the substrates when compared with the wild type. …”

T-cell receptor signaling induces proximal Runx1 transactivation via a calcineurin–NFAT pathway by Wong, W.F., Looi, C.Y., Kon, S., Movahed, E., Funaki, T., Chang, L.Y., Satake, M., Kohu, K.

“…In a luciferase reporter assay, this region was found to be responsive to T-cell activation through Lck and calcineurin pathways. Mutagenesis studies and chromatin immunoprecipitation assay indicated that the NFAT site was essential for NFAT binding and transactivation of the proximal Runx1 promoter. …”

VP1 residues around the five-fold axis of enterovirus A71 mediate heparan sulfate interaction by Tan, C.W., Sam, I.C., Lee, V.S., Wong, H.V., Chan, Y.F.

“…With In silico heparin docking and mutagenesis of all possible lysine residues in VP1, we identified that K162, K242 and K244 are responsible for heparin interaction and inhibition. …”

Homology modeling of the human 5-HT(1A), 5-HT (2A), D1, and D2 receptors: model refinement with molecular dynamics simulations and docking evaluation by Yap, B.K., Buckle, M.J., Doughty, S.W.

“…Docking evaluations with representative agonists and antagonists using AutoDock 4.2 revealed binding modes in agreement with experimentally determined site-directed mutagenesis data and significant correlations between the computed and experimental pK (i) values. …”

Functional identification of the prnABCD operon and its regulation in Serratia plymuthica by Liu, Xiaoguang, Yu, Xiaoli, Yang, Yang, Heeb, Stephan, Gao, Shao, Chan, Kok Gan, Cámara, Miguel, Gao, Kexiang

“…Further experiments involving lux-/dsRed-based promoter fusions, combined with site-directed mutagenesis of the putative σS extended -10 region in the prnA promoter, and liquid chromatography-mass spectrometry (LC-MS) analysis extended our previous knowledge about G3, revealing that quorum sensing (QS) regulates PRN biosynthesis through cross talk with RpoS, which may directly activated prnABCD transcription. …”

Dechlorination of polychlorobiphenyl degradation metabolites by a recombinant glutathione S-transferase from Acidovorax sp. KKS102 by Shehu, Dayyabu, Alias, Zazali

“…Bioinformatic analysis indicated several conserved amino acids that participated in the catalytic activity of the enzyme, and site-directed mutagenesis of these conserved amino acids revealed their importance in the enzyme's catalytic activity. …”

Banana, Gingers and Papaya Cell Cultures for High Throughput Agriculture by Khalid, Norzulaani, Chee, W.W., Bokhari, F., Kiat, T.S., Idris, F.D., Mohamad, M., Ibrahim, H., Annuar, S., Othman, R.Y., Rahman, N.A.

“…Besides being able to produce high-quality planting materials, plant cell cultures also allow genetic manipulation for trait improvement through either genetic engineering or mutagenesis (mutation breeding). Metabolomics is another possible endeavour in an attempt to enhance the production of certain fine chemicals in plants. …”
Get full text