Cultivating conditions influence invertase production by Aspergillus Niger in submerged culture by Mohamad Izhar, Mohd Samin

“…Studied conducted on one factor at a time yield maximum amount of invertase activity at 30 g/L sucrose concentration,pH 5.5 and 250 rpm with amount of 8.9132 IU/mL,8.6754 IU/mL and 8.3429 IU/mL respectively after 40 hours fermentation period. …”
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Biological hydrogen production from Palm Oil Mill Effluent (POME) by Yeap, Shu Ying

“…The results showed the optimum condition was 35˚C and pH 5.5. The COD removal of Bacteria JTY2017 was 39%. …”
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Isolation of albumin protein from goat�s milk using anion exchange chromatography by Gaase, Poarasy

“…Milk proteins have been extensively studied for over 90 years.The aim of this study was to introduce a simple, reproducible,and less expensive method which is ion exchange chromatography for isolation of albumin protein from goatâ��s milk.The main whey proteins are β-lactoglobuline and α- lactalbumin for which genetic variants are also known.The fraction of whey also contains substantial amounts of immunoglobulin and serum albumin.Whey (lactoserum) was obtained by isolating casein from defatted milk using hydrochloric acid.The proteins in the supernatant were fractionated using Q-Sepharose column chromatography for separation.This research were conducted to optimize the pH of the milk sample for separation process.The processes of the separation initialized with sample preparation followed by packing process of ion exchange column,buffer preparation,ion exchange running process and ending by analyzing the ion exchange fraction using reverse process chromatography (PRC).The pH that have been analyzed during this study were milk with pH 5.5, 6.5, 7.5 and 8.5.Result shows that ph 8.5 able to collect α-lactalbumin whereas in other pH does not so.Anion exchange chromatography able to isolate BSA and higher concentration was obtained at pH 5.5 with the amount, 0.03616164 mg/ml.RPC method is presented for the determination of albumin method is rapid, reliable and sensitive and gives good accuracy and precision for measurement of the whey proteins.The procedure developed is particularly suited to laboratory-scale applications,but it can also be applied to the purification on a large scale. …”
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Effect of pH and Temperature on Fructosyltransferase (Ftase) Production from Candida Krusei by Mohd Nazarni, Che Isa

“…As result indicates that the highest pH and temperature for activity of FTase from Candida krusei is pH5.5 and temperature 55oC. The highest enzyme activity (148.501 IU/mL) was figured out at pH 5.5, 55oC and the specific activity was observes as 10.638 IU/mg. …”
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Optimization of yellow pigment production by Monascus purpureus from banana peel by Nur Ashidah, Abdul Satapa

“…By the point prediction tool of Design-Expert 6.7, the optimum values of the factors for maximum pigment production were determined: initial substrate moisture content 57.5%, pH 5.5 and temperature 40˚C. With this point optimization, the pigment yield for yellow pigment is 2.2104 OD/gram dry solid was closely to 2.3754 OD/gram dry solid with is the predicted yield.…”
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Selective oxidation of glycerol to mesoxalic acid by laccase/2,2,6,6-tetramethylpiperidine-N-oxyl system : effect of process conditions and the kinetic modeling by Hong, Chi Shein, Chin, S. Y., Cheng, C. K., Chua, Gek Kee

“…Mesoxalic acid attained was 0.0712 M (>200% of control) at 19 °C, pH 5.5, 1:3 of glycerol/TEMPO ratio and TEMPO/laccase ratio of 9:3. …”
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Biosorption of lead (II) from aqueous solutions by dried water hyacinth (Eichhornia Crassipes) by Siti Aishah, Abd Wahid

“…From the result obtained, the removal of Pb (II) ions was optimum when initial lead concentration was almost 40 mg/ L, biosorbent dosage at 0.225 g, pH 5.5 and at 8 minutes contact time. Other process conditions were optimized. …”
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Optimum conditions for sucrose production using ultrasonic treatment by Tea, Lei Kwan

“…The coconut sugar concentrations that were used in this study were 40, 60 and 80 % w/v, diluted in pH 5.5 acetate buffer solution. Ultrasonic frequencies of 25, 68 and 132 kHz were used to treat these coconut sugar concentrations for 5 hours with the samples being analyzed every 30 minutes. …”
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Enzymatic production of fructooligosaccharides from phytoenzymes of ananas comosus waste by using response surface methodology: one factor by Nur Dini, M. J., Noormazlinah, Ahmad, Norhanimah, Hamidi, Nurul Aini, Mohd Azman

“…As a result, an optimum condition FOS production was obtained are, time of reaction (100 minutes), temperature (60 ̊C), pH (5.5), sucrose concentration (60%w/v), and phytoenzymes concentration (30% w/v), respectively and producing 150.456 (g/ml).This investigation can be further extended to explore the prebiotic potential of synthesized FOS.…”
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Bacterial cellulose production from food waste by Chua, Gek Kee

“…The optimal operating conditions to produce BC were found to be at 30C, pH 5.5, 10% inoculum density and 150 rpm stirring speed. …”
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Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent by Nor Hazwani Syuhada, Azmi

“…For the immobilization process, it was occurred in water bath shaker for 26 h at room temperature and at pH 5.5. The enzyme activity was analyzed by using DNS method. …”
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Production of Bioethanol from Kundur [Benincisa Hispida (Thunb) Cogn] by Siti Salbiah, Mutalib

“…The fermentation process was carried out in duplicate at 30°C, pH 5.5 and 200rpm for 72hours. According to the results obtained, the ethanol yield for the fermentation process without nutrient supplement is in the range of -0.192~ -0.194 (g ethanol/g biomass) while the specific growth rate is in the range of -0.0148~ -0.015h-1. …”
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Accelerated two-stage bioprocess for hydrogen and methane production from palm oil mill effluent using continuous stirred tank reactor and microbial electrolysis cell by Krishnan, Santhana, Mohd Fadhil, Md Din, Shazwin, Mat Taib, Mohd Nasrullah, Zulkifli, Mimi Sakinah, A. M., Zularisam, A. W., Kamyab, Hesam, Chelliapan, Shreeshivasan, Rezania, Shahabaldin, Singh, Lakhveer

“…CSTR reactor was operated at pH 5.5, 80 rpm, 2 days HRT, 60 g COD L-1 d-1 organic loading rate (OLR) and 55 °C temperature with the given hydrogen yield of 205 ml H2 gCOD-1along with acetic, butyric, propionic, and lactic acid as by-products. …”
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Enhancement of Biocellulose Production in Mixed Medium Culture by Junaidi, Zakaria, Shahril, Mohamad, Siew, Kah Weng

“…In this experiment, it was ensured that all the samples had the initial temperature and pH of 30ºC and pH 5.5 respectively. The temperature was kept constant throughout the whole experiment while the changed pH value was taken as final pH at the end of the experiment for every sample. …”
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Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time by N. A. S. M., Rosdee, N., Masngut, S. M., Shaarani, S., Jamek, M. S. M., Sueb

“…The result has shown that the highest reducing sugar concentration of 70.9 mg/L was obtained both at pH 5.5 and 50 °C. In addition, the best enzyme loading and reaction time were recorded at 0.5% (w/v) and 45 min that resulted 60.1 mg/L and 72. 0 mg/L respectively.…”
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Enhancement of biocellulose production in mixed medium culture by Kah, Weng Siew

“…Biocellulose production was determined by utilizing different feedstocks of single sugars and sugar mixtures which were applied according to certain glucose to fructose ratio.Data for pH changes and the biocellulose production from every medium culture were thoroughly analyzed.In this experiment,it was ensured that all the samples had the initial temperature and pH of 30ºC and pH 5.5 respectively. The temperature was kept constant throughout the whole experiment while the changed pH value was taken as final pH at the end of the experiment for every sample.The highest production using sugar mixtures of 1:9 glucose to fructose ratio was 1.57g/L.The final pH values recorded in the different sugar mixtures were in the range of 4.0–5.5.The lowest final pH of 4.56 was determined in the medium that contained a single carbon source of glucose, as most of the glucose was converted into gluconic acid and lead to lowest biocellulose production of 0.69g/l.In contrast, the highest pH value of 5.3 was determined in the medium that contained a single carbon source of fructose and lead to the higher biocellulose production of 0.9g/l.Analyzing profiles for final pH and biocellulose production for the medium with higher glucose concentration showed that the glucose was preferable to be converted to gluconic acid rather than biocellulose synthesize.Besides,it was also determined that biocellulose production rate in mixed culture medium was higher than in culture medium that only consist of a single carbon source and this had proved that the experiment of enhancing biocellulose production with mixed medium culture was applicable.Results reported in this study demonstrated that the production of biocellulose can be enhanced by using carbon sources mixture with a suitable ratio.This not only represented that biocellulose would be a renewable source of cellulose in the future,but also might lead to major improvements in production if proper supplements and control were utilized in the fermentation process.…”
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Production of fructo-oligosaccharides from the enzymatic conversion of coconut sugar catalyzed by fructosyltransferase in a batch reactor by Noormazlinah, Ahmad

“…The molecular weight of FTase is 142 kDa with 401 U/mL of enzyme activity while it is more stable between 50 to 60 oC and pH 5.5. The experimental work initially has been carried out with six reaction parameters (coconut sugar concentration, enzyme concentration, temperature, pH level, reaction time and agitation speed) was performed to facilitate the appropriate range prior to conducting a fractional factorial design approach. …”
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Production of mesoxalic acid from glycerol oxidation by laccase/2,2,6,6-tetramethylpiperidine-1-oxyl (LACASSE/TEMPO) system : effect of process parameters and kinetic study by Hong, Chi Shein

“…The best reaction conditions after conducting experiments were found to be 19 °C, pH 5.5, ratio 1:3 of glycerol to TEMPO and ratio 9:3 of TEMPO to laccase. …”
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Optimization of xylonic acid production using recombinant e. coli bl21 (de3) with inserted gene from ralstonia pickettii by Nurul Atikah, Mohd Rodzri

“…In screening, temperature, initial pH of medium and agitation rate were varied from 25°C to 40°C, pH 5.5 to pH 8.5 and 50 rpm to 250 rpm respectively. …”
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