Development of nested polymerase chain reaction for detection of rabies by Fazilah Muhamad Alwi, Musherah Khusaini, Ag. Muhammad Sagaf Abu Bakar, Ainol Azifa Mohd Faik

“…The aim of this study was to develop a nested reverse-transcriptase polymerase chain reaction (nRTPCR) for the detection of this virus along with a canine internal control to monitor the integrity of the extracted RNA. …”
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Development of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) based techniques for meat identification by Lim, Chelven Ai Chen

“…This technique is based on Polymerase Chain Reaction-Restriction Fragment length Polymorphism (PCR-RFLP) of a conserved region in the mitochondrial (mt)cytochrome b (cyt b) gene for meat identification and authentication. …”
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Screening the occurrence of Grouper Iridovirus (Giv) in cage cultured Groupers in Sabah using Polymerase Chain Reaction (PCR) Technique by Julian Ransangan

“…Total genomic DNA from grouper larvae were extracted and subjected to polymerase chain reaction using specific primers targeting two genes, which are the major capsid protein and the ATPase genes of grouper iridovirus. …”
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Development Of Rapid Detection System For Pathogenic Vibrio Harveyi In Sea Bass, Groupers And Shrimps by Julian Ransangan

“…Several diagnostic techniques have been developed for Vibrio harveyi including the use of biochemical tests, culture of the bacterium on selective medium and the use of polymerase chain reaction (PCR) that are targeting different genes such as 16S ribosomal DNA and toxin gene (toxR). …”
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Development of insulated isothermal PCR for rapid on-site malaria detection by Kek Heng Chua, Chin Lee, Ping, Hwa Chia Chai

“…In this study a portable diagnostic test of Plasmodium spp. was developed using insulated isothermal polymerase chain reaction (iiPCR) as an alternative approach to improve this situation. …”
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Effectiveness of DNase and washing steps in removing dead cells’ DNA for PCR detection of viable Escherichia coli by Ho, C. F., Teoh, Peik Lin, Vun, L. W.

“…This study investigates the use of DNase and washing steps in removing dead cells’ DNA during sample preparation and their effect on the detection of viable cells using polymerase chain reaction (PCR). The results indicated that the DNA from heat-killed cells could be completely removed by DNase; thus, would not be detected by PCR. …”
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Genetic data for 15 STR loci in Kadazan-Dusun population from East Malaysia by Kee, Boon Pin, Lian, L. H, Lee, Ping Chin, Lai, T. X, Chua, Kek Heng

“…All loci were amplified by polymerase chain reaction, using the Powerplex 16 system. Alleles were typed using a gene analyzer and the Genemapper ID software. …”
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Detection of viable bacteria in environmental water samples using DNase I and PCR method. International Journal of Environmental Studies by Teoh, Peik Lin, Ho, C. F., Vun, L. W.

“…To increase the sensitivity of the method, and the recovery of microbial cells, water samples were filtered before being subjected to DNase treatment and polymerase chain reaction amplification. Results showed that DNase I treatment and PCR reaction were not affected by inhibitors as the expected amplicon was successfully amplified in autoclaved environmental waters spiked with E. coli. …”
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Occupational Determinants of Leptospirosis among Urban Service Workers by Azman Atil, Mohammad Saffree Jeffree, Syed Sharizman Syed Abdul Rahim, Mohd Rohaizat Hassan, Khamisah Awang Lukman, Kamruddin Ahmed

“…Information on demography, occupational exposures and environmental factors was obtained by a modified validated questionnaire. Polymerase Chain Reaction (PCR) was used to determine the prevalence of positive leptospirae. …”
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Genotyping for polymorphism of STK-15, a low-penetrance gene in colorectal cancer by Ng, Chen Seng

“…To examine whether functional variation in STK-15 may affect the susceptible risk, DNA samples from colorectal cancer patients (n = 11) and healthy individual (n = 1) were genotyped for Phe31IIe polymorphisms. Polymerase chain reaction-restriction fragment length polymorphism was used in this study. …”
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Tetraplex PCR assay involving double gene-sites discriminates beef and buffalo in Malaysian meat curry and burger products by M.A. Motalib Hossain, Md. Eaqub Ali, Sharifah Bee Abd Hamid, S.M. Azad Hossain, Asing, Nina Naquiah Ahmad Nizar, Mohammad Nasir Uddin, Lokman Ali, Md. Asaduzzaman, Md. Jahurul Haque Akanda e

“…For the first time, we report a tetraplex polymerase chain reaction (PCR) assay targeting two different DNA regions in beef (106 and 120-bp) and buffalo (90 and 138-bp) mitochondrial genes to discriminate beef and buffalo in processed foods. …”
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Genotyping of XRCC1 polymorphisms in breast cancer by Nalini Krishnan

“…Arg194Trp polymorphism at the XRCCI gene were analyzed by using Restriction Fragment Length Polymorphisms (RFLP) assay based on DNA samples of five patients with breast cancer and one healthy subject. Polymerase Chain Reaction (PCR) was carried out by using specific primers to amplify the region XRCCI containing Argl94Trp site. …”
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Effectiveness of DNase and washing steps in removing dead cells' DNA for PCR detection of viable Escherichia coli by C. F. HO, Peik Lin Teoh, Leong wan Vun

“…This study investigates the use of DNase and washing steps in removing dead cells’ DNA during sample preparation and their effect on the detection of viable cells using polymerase chain reaction (PCR). The results indicated that the DNA from heat-killed cells could be completely removed by DNase; thus, would not be detected by PCR. …”
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Genetic transformation of Labisia Pumila using Agrobacterium Tumefaciens by Ainul Mardziah Mohamed

“…Expression and presence of transgene was assayed by histochemical test and later confirmed with polymerase chain reaction. Seven percent transgenic plants were micropropagated and successfully acclimatised. …”
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Genotyping of Salmonella spp. on the basis of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) by Calvin Jiksing, Normah Yusop, Farhan Nazaie Nasib, Kenneth Francis Rodrigues

“…Deoxyribonucleic acid (DNA) was extracted and polymerase chain reaction (PCR) was performed using primers which were designed to amplify the CRISPR array in Salmonella genome. …”
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Effects of Testosterone on the Expression of Connexin 26 and Connexin 43 in the Uterus of Rats During Early Pregnancy by Datu Agasi Mohd Kamal, Siti Fatimah Ibrahim, Mohd Helmy Mokhtar

“…The rats were sacrificed at day 4 of pregnancy, which was considered as the uterine receptivity period for determining the expression and distribution of connexin 26 and connexion 43 by immunohistochemistry and quantitative polymerase chain reaction, respectively. Results: Treatment with 1 mg/kg/day testosterone increased connexin 26 and decreased connexin 43 mRNA expression and protein distribution in the uterus of early pregnancy rats. …”
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Weissella sp. Taj-Apis, a novel lactic acid bacterium isolated from honey by Naser Tajabadi, Makhdzir Mardan, Shuhaimi Mustafa, Faegheh Fe, Leila Nateghi, Yazid M Abd Manap, Babak Rasti

“…The 16S rRNA genes from extracted DNA of bacterial colonies were amplified with polymerase chain reaction (PCR) using universal primers (27F and 1492R). …”
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Screening of differentially expressed Gene(S) and low Penetrance Cancer Susceptibility genes in Colorectal Cancer for Biomarker development by Lee, Ping Chin, Chua, Kek Heng

“…Using STK1S and polymerase chain reaction technology, a kit was developed to detect colorectal cancer in this project. …”
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A multiplex PCR method for rapid identification of commercially important seaweeds Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum (Rhodophyta, Solieriaceae) by Vun, Yee Thien, Thau, Wilson Lym Yong, Ann Anton, Grace Joy Wei Lie Chin

“…In this study, we have developed a simple, efficient and reproducible one-step polymerase chain reaction (PCR) based method for the identification of the three eucheumatoid species, K. alvarezii, K. striatus and E. denticulatum. …”
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Mutagenic effects of ethyl methanesulfonate on nine protein coding genes in tomato (Solanum lycopersicum L.) by Wilson Thau LymYong, Aswaneeza Khairul Aswandy, Bo, Eng Cheong, Kenneth Francis Rodrigues

“…Mutants were selected based on phenotypic variation, following which nine specific genes such as NRP2 (RNA polymerase II), HKX (hexokinase), P2D1 (photosystem II D1), NEXP (expansin), PHYSN (phytoene synthase), PCANH (carbonic anhydrase), NAGO (argonaute), PRUB (RuBisCO) and NGAG (gag-pol) were isolated via polymerase chain reaction and sequenced to determine the presence of mutations. …”
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